rev: October 4, 2001
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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
-clone G186-1117, mIgG1
-immunogen: a recombinant human 5HT2AR fusion protein (amino acids 1-72)
-reacts with human, rat and monkey 5-HT2AR
-suitable for western blot (recognizes the 5HT2AR as approx 55Kd band and histochemistry (frozen tissue sections)
-ref: J. Biol Chem 272:14825-14829
J. Histochem Cytochem 46:811-824
Brain Res 763:271-275
Mouse anti-Serotonin Receptor 5-HT2BR
-clone A72-1, mIgG1
-immunogen: a recombinant N-terminal 5-HT2BR fusion protein
-reacts with human and rat 5-HT2BR
-suitable for western blot (recognizes the 5HT2BR as approx 55Kd band using rat brain synaptic plasma mebrane) and histochemistry (frozen tissue sections, pos control rat brain/hippocampus tissue)
Mouse anti-Serotonin Receptor 5-HT2CR
-clone A4-2, mIgG1
-immunogen: a recombinant C-terminal 5-HT2R fusion protein
-reacts with human and rat 5-HT2CR
-suitable for western blot (recognizes the 5HT2CR as approx 55Kd band using rat brain synaptic plasma membrane) and histochemistry (frozen tissue sections, pos control rat brain/cerebellum , staining Purkinje cell bodies and dendrites)
cat #RDI-5HT2BRabm $469.00/100ug vial
Size: 0.1 mg in 0.2ml 50mM TRIS-HCL pH 8.0 with 150mM NaCl and 0.09% sodium
Isotype: mouse IgG1
Immunogen: a recombinant fusion protein between GST and human 5-HT2BR
Format: Purified by Protein G
Reactivity: human and rat 5-HT2BR (tested and found non reactive with GST)
Use: -western blot (recognizes the 5HT2BR as approx 55kda band using rat brain synaptic plasma membrane) approx 1ug/ml for 2-3 hours RT or overnight 4 DEg C with chemiluminescence detection)
-histochemistry: frozen sections 2-10ug/ml (pos control rat brain tissue/hippocampus
Background: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor, plays an important role in regulating diverse biological processes in the central and peripheral nervous systems, the cardiovascular system and the fastrointestinal systems. The different functions of serotonin are thought to be mediated through specific receptors. Physiological, biochemical,and pharmacological studies have defined at least thirteen serotonin receptors. The thirteen receptors are grouped in seven subtypes according to operational, structural, and transductional properties. The 5-HT2 receptors (5-HT2AR, 5-HT2BR,and 5-HT2CR) may be considered as prototypes for the study of serotonin receptors. Their functions are mediated by the second messenger inositol polyphosphate in a G- protein coupled pathway. They are expressed in diverse tissues including brain, vascular smooth muscles, and platelets (reviewed in 3). However, the exact localization of 5-HT2 receptors in the CNS remains to be elucidated. The 5-HT2AR mRNAs have been found in only a few areas of the brain, including the frontal cortex, piriform cortex, ventro-caudal part of CA3, motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal cord. The 5-HT2CR mRNA has been found at high levels in many parts of the brain including retrosplenial piriform, and entorhinal cortex, anterior olfactory nucleus, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. The 5-HT2BR is almost absent in the brain, but is abundantly expressed in stomach fundus. Monoclonal antibodies reacting specifically to 5-HT2AR, 5-HT2BR, and 5- HT2CR will be useful tools in determining the precise anatomical distribution of 5-HR2 receptors.
1. Guillet-Deniau, I., A.F.Burnol and J. Girard. 1997. Identification and
localization of a skeletal muscle serotonin 5-HT2A receptor coupled to the
Jak/STAT pathway. J.Biol.Chem.272:14825-14829.
2. Wu,C.,E.J. Yoder,J.Shih,K. Chen,P. Dias,L.Shi,S.D.Ji, J. Wei,J.M.
Conner,S.Kumar, M.H. Ellisman and S.K.Singh. 1998. Development and
characterization of monoclonal antibodies specific to the serotonin 5-HT2A
receptor. J. Histochem. Cytochem. 46:811-824.
3. Saudou,F. and R. Hen.1994. 5-Hydroxytryptamine receptor subtypes in
vertebrates and invertebrates. Neurochem. International 25:503-532.
4. Humphrey,P.P.,P. Hartig and D. Hoyer.1993. A proposed new nomenclature for 5-
HT receptors. TIPS. 14:233-236.
5. Pompeiano,M., J.M. Palacios and J. Mengod. 1994. Distribution of the serotonin
5-HT2 receptor family mRNAs: comparison between 5-HT2A and 5-HT2C receptors.
Mol.Brain Res. 23:263-178.
6. Carlton,S.M. and R.E. Coggeshall. 1997. Immunohistochemical localization of 5-
HT2A receptors in peripheral sensory axons in rat glabrous skin. Brain Res.
7. Willins,D.L.,A.Y. Deutch and B.L. Roth.1997. Serotonin 5-HT2A receptors are
expressed on pyramidal cells and interneurons in the rat cortex. Synapse 27:79-
8. Jakab,R.L. and P.S. Goldman-Rakic.1998. 5-Hydroxytryptamine2A serotonin
receptors in the primate cerebral cortex: possible site of action of
hallucinogenic and antipsychotic drugs inpyramidal cell apical dendrites.
Proc.Natl.Acad.Sci. USA 95:735-740.
9> Duxon et al, Evidence for expression of the 5-hydroxytryptamine-2B receptor
protein in the rat central nervous system. Neurosci 76:323-329 (1997)
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