rev:       September 8, 2004

HOME (index page)

Return (alphabetical antibody index page)

Return  (neurohistology ab index page)


ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


S100 (see below 6 monoclonals and polyclonals)
Monoclonal mouse anti-human S-100 protein

Catalog # RDI-TRK4S37    $438.00/1mg   $375.00/mg 10+ or more Bulk Quotes on Request

-see also s100.pdf

Clone: clones 8B10, 6G1, 3B10, 4B3

These clones have been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with human S-100 protein.

Specificity: Clone 8B10, 6G1, 4B3 , specific to S-100ab and S-100bb

                    Clone 3B10 - specific to S100ab

Mouse isotype: clones: 8B10, 6G1- IgG1,    clones 4B3, 3B10 , IgG2a

Application: S-100 immunoassay and immunoaffinity purification. All clones are working in Western blotting.

Recommended pairs for sandwich immunoassay: (coating - conjugate)

Best Pair: 8B10-6G1

other pairs:  3B10 - 6G1,

-specificty in western blot
MAB Clone Isotype Specificity in western blot cat#
3B10 IgG2a Alpha-Beta RDI-TRK4S37-3B10
8B10 IgG1 Alpha-Beta and Beta-Beta RDI-TRK4S37-8B10
6G1 IgG1 Alpha-Beta and Beta-Beta RDI-TRK4S37-6G1

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7.4 containing 0,1% of sodium azide as preservative. Store at +4C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.


Human S-100 Antigen (Beta-Beta homodimer and Alpha-Beta heterodimer)

CAT#: RDI-S100-AG     $406.00/0.1mg   $750.00/0.5mg    $1250.00/1mg  

Package: Each vial contains 1.0mg lyophilized

Source: Human brain tissue serum. Blood sample from tissue donor was tested and found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.

Presentation: Lyophilized from DI water with 5mM 2- mercaptoethanol and 5mM EDTA. It is recommended that the product should be reconstituted in 1ml 5mM 2-mercaptoethanol solution.

Analysis: Purity more 98% according to SDS-PAGE (Native gel electrophoresis, 20% Page with 2mM EGTA, stain coomassie blue R-250) MW approx 21kda,

Storage: At 4 DEG C or below in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.

For In Vitro Research Use Only-Not for Use in or on human or animals-not for use in diagnostics

Background: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kda. In human brain tissue, S-100 protein is mainly presented by two isoforms, a Beta-Beta(BB) homodimer or S-100b and an Alpha-Beta (AB) heterodimer or S-100a. Becasue of its predomiant location in astrological cells, S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples may eb useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, in    diagnosis and prognosis of clinical outcome in acute stroke.

Warning: Prepared from human tissue; As no method can assure complete non-infectivity, material should be handled under Biosafety Level II (CDC) condition and treated as if capable of transmitting infection.


cat#RDI-TRK8S92h   ( Human S-100 Protein BB homodimer)

$406.00/0.1mg  $750.00/0.5mg  $1250.00/1mg   $1000.00/mg 2+


cat#RDI-TRK8S92b   ( BOVINE S-100 Protein BB homodimer)

$406.00/0.1mg   $750.00/0.5mg   $1250.00/1mg    $1000.00/mg 2+


Mouse anti-S100

cat# RDI-S100abm      $312.00/100ug

       RDI-S100abm-1   $438.00/200ug

Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified bovine brain S100

Isotype: mIgG2a

Clone: 4C4.9

Reactivity: human, bovine, mouse

Uses: -Western blot: approx 0.5ug/ml

        -histochemistry (frozen and paraffin sections) approx 1:100-1:200 60 minutes RT

Positive Control melanoma or Schwannoma

Localization: Cytoplasmic

Background: Recognizes proteins of 21-24kDa, identified as the A & B subunits of S100 protein. S100 belongs to the family of   calcium binding proteins such as calmodulin and trop0onin C.   S100A is composed of an alpha and beta chain whereas S100B   is composed of two beta chains. Antibody to S100 stains Schwnnomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metasases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. The diagnosis of Histocytosis X is confirmed by S100 staining. S100 protein is highly soluble and may be eluted from frozen tissue during staining.

Storage: 2-8 deg c

For Research Use only


Rabbit anti-S100B

Catalog#: RDI-S100Babr

Price: $440.00/vial

Packaging: 200 microliters lyophilized antiserum in 15mM KPO4 ppH 8, approx 43mg/ml IgG, no preservatives)

Reconstitution: with 200ul distilled water

Species: rabbit

Antigen: purified bovine brain S100B

Specificity: specific for S100B polypeptide, showing <0.5% cross reactivity with S100A(alpha) and no cross reactivity with calmodulin. The antibody reacts with S100B from all species tested to date including bovine, rat, chicken, and human.

Uses: western blot: 1:200-1:1500

          Immunohistochemistry: 1:5000- 1:10K with avidin biotin method

         Elisa:

         RIA:1:1000

Optimal dilutions must be determined by the end user for their particular application.

ref: -Van Eldik L.J. and Griffin WST (1994), Biochim. Biophys Acta 1223:398-403

       -J. Neurochem 65:228-233

Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT FREEZE THAW CYCLES.

Allow 2 weeks for delivery:

Additional product of interest: recombinant human S100B cat#RDI-S100B-AG $220.00/10ug

-For Research Use Only  


ANTIBODIES AGAINST S-100


cat#RDI-S100abg            Goat anti-S100  $312.00/1ml

-made against bovine brain S-100 purified by SDS-Page

-suitable for immunohistochemistry and EIA

-sold as 1ml neat serum

-no other data available


cat#RDI-S100Labm     mouse anti-human S100L

$438.00/50ug   $750.00/150ug

-made against bovine S100L

-mIgG1 packaged at 250ug/ml in 50% glycerol:PBS

-reacts with human S100L in immunofluorescence and in  western blot (10kda using MDBK cells)


Mouse anti-S100P Monoclonal ab

cat# RDI-S100Pabm      $438.00/vial 50ug

       RDI-S100Pabm-1   $750.00/vial 150ug

Background: The S100 multigene family consists of a number of small dimeric   molecules around 100 residues in length. The original mmbers of this family, S100alpha and S100Beta were initially identified in bovine brain. S100P is a novel member of this family ehich was isolated from human placenta. The S100 family consists of at least 10 members which show a cell-type-specific expression pattern. Most S100 proteins are believed to be involved in cytoplasmic Ca+ control. S100P consists of 95 amino acids and shares about 50% sequence identity with S100alpha and S100Beta. Like other S100 proteins, S100P functions as a homodimer and binds to Ca2+ ions per monomer. Each S100P molecule contains botha low affinity and a high affinity Ca2+ binding site.

Antigen: derived from human S100P

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: Suitable for western blot on human using approx 1ug/ml ab on total cell lysate. Major band at Mw 11kDa. Positive control human placenta lysate. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Becker, T et al, 1992. Eur J Biochem 207:541

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner

Recommended positive western blotting Control:Human Placenta Lysate

Prepared from lysis and heat denaturation of human placenta in 1% SDS, 1.0mM sodium vanadate, 10mM Tris 7.4.

with enhanced chemiluminescence detection. Do Not Boil or Dilute Before Use.

additional cell lysates available on request  cat#RDI-HPL/CPL $250.00/500ug vial

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS.

see also antibodies to calmodulin and related products-please inquire.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Ordering terms

RETURN(neurohistology ab page)