rev:     May 15, 2007

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

+++ = (almost) all acid phosphatase-positive cells stained with the monoclonal antibody

++ = A considerable number stained   += few stained   -/+ very few stained or none at all

-distribution and staining pattern of macrophages identiifed by ED1, ED2 and ED3 in various organs (from Djkstra et al., 1985, modified)

-clone Ki-B1R is useful as a pan-B cell marker for detecting B-lymphocytes and plasma cells in cell suspensions and sections of rat tissues

-frozen and paraffin,and facs

-mIgG1

-cat#RDI-T3106X   $531.00/50ug

Monoclonal Antibody  Ki-B1R

Product Information

Anti Rat B-Cells

Ki-B1R is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and cryostat sections of rat tissues. It was generated through fusion of mouse Ag8-653 myeloma cells with splenocytes of mice immunized against rat lymphocytes.

Application: Immune histology on frozen and paraffin embedded tissues.

Lot number:

TECHNICAL CHARACTERISTICS

Species: Mouse

Class: IgG1

Quantity: 50ug

Purity: Affinity purified

Physical state: Lyophilized powder

Reconstitute in: 0.5ml distilled water (=isotonic stock solution)

Buffer: Phosphate buffered saline, pH 7.2

Preservative: 0.01% Thimerosal

Stabilizer: 10mg/ml BSA

Storage: Aliquots of stock solution can be kept frozen at -20°C;  do not freeze working dilutions.

Approximate working dilution: 1mg/ml (1:100) freshly prepared

For laboratory use and research purposes only

This antibody has been shown to react on paraffin sections without particular antigen retrieval, and on sections after microwave treatment.

Caution: this product contains Thimerosal, a poisonous and hazardous substance

BIOLOGICAL CHARACTERISTICS

SPECIFICITY Rat: mature B-lymphocytes, plasma cells

Other species: unknown

ANTIGEN DISTRIBUTION

Isolated cells:

In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% + 3% of peripheral blood lymphocytes (n=5). In the same test, 18% ± 3% of the lymphocytes stained positive for sIg, whereas 79% ± 3% stained positive with Ki-T1R, the rat pan T-cell marker.

Tissue sections:

Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo.

BIOCHEMISTRY

Ki-B1R recognises a surface antigen of 47kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope is stable for short (5 secs) acetone and paraformaldehyde fixation.

SUGGESTED CONTROL

Unfixed spleen sections

-clone Ki-T1R is a pan T-cell marker and useful for the characterization of lymphoid organs and for the study of the interaction of T-cells with other cell types, eg macrophages

-suitable for histochemistry (frozen and glutaraldehyde fixed & paraffin), FACS

-cat#RDI-T3122X      $531.00/200ug

Monoclonal Antibody  Ki-T1R Biotin

Product Information

Anti Rat T-Cells

BMA Ki-T1R as a pan-T-cell marker is a useful reagent for the characteristion of lymphoid organs and for the study of the interaction of T-cells with other cell types e.g. macrophages.

Applications: Immune histology, FACS

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG2a

Purity: Purified IgG, biotinylated

Physical state: Lyophilized

Quantity: 200ug

Buffer: Phosphate buffered saline

Stabilizer: 10 mg/ml bovine serum albumin

Preservative: 0.01 % Thimerosal

Reconstitute in: 0.5 ml distilled water (=stock solution)

Storage: Aliquots of stock solution can be kept frozen at -70°C; do not freeze working dilutions!

Stability of stock solution: 1 year at -70°C

Approximate working dilution: dependent on the system used

Histology: 1mg/ml (1:400) freshly prepared!

Fixation: Acetone, Glutaraldehyde, Formaldehyde

For laboratory use and research purposes only

Caution: this product contains Thimerosal a poisonous and hazardous substance

References:

Data obtained in the laboratories of Pathology Kiel, Germany

-useful for characterizing macrophage subpopulations of various tissues. Up to 80% of bronchial lavage cells and 90% of activated peritoneal cells are recognized by this clone.

-ascites, mIgG2a, suitable for acetone fixed cells

-ref: Immunology 57, 239 (1986)

-cat#RDI-T3002X   $406.00/0.25ml vial

Monoclonal Antibody OX-41

Product Information

Anti Rat CD172a, Signal Regulatory Protein

OX-41 recognises rat SIRP which is expressed selectively by myeloid cells and neurons. It is a useful marker for characterising macrophage subpopulations of various tissues. In combination with other macrophage markers like OX-42 it allows a detailed phenotyping of specific macrophage subsets.

Applications: Immune histology, FACS, Western Blots

Lot number:

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG2a

Purity: Purified IgG

Physical state: Liquid

Volume / concentration: 0.25ml / 1mg/ml

Preservative: 0.1% Sodium azide

Storage: Aliquots of stock solution can be kept frozen at -20°C;

Avoid repeated thawing and refreezing!

Stability of stock solution: 1 year at -20°C

Approximate working dilution: 1:50 - 1:100

This product requires protease treatment for the staining of paraffin sections.

For laboratory use and research purposes only

Caution: this product contains sodium azide, a poisonous and hazardous substance.

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Rat: granulocytes, monocytes, macrophages

Other species: unknown

BIOCHEMISTRY

OX-41 precipitates a surface antigen which migrates as a broad band (110-120kD) under reducing or non-reducing conditions.

ANTIGEN DISTRIBUTION

Isolated cells:

Up to 80% of bronchial lavage cells and 90% of activated peritoneal cells are recognised by OX-41. Granulocytes and monocytes are also positive with OX-41.

Tissue sections:

OX-41 detects a wide range of macrophages in various tissues. It is especially suitable for the detection of follicular tingible body macrophages. In the brain a diffuse staining of brain tissue similar to Thy-1 marker was observed along with a distinct staining of glial cells. Only a few Kupffer cells are recognised by OX-41 in the liver. Side reactions with interstitial cells of the small intestine were reported. Such side reactions were absent in the kidney and heart.

SUGGESTED CONTROL

Frozen sections of rat spleen

SELECTED LITERATURE

Robinson, A.P. et al.: Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRCOX-41 and MRCOX-42, the latter recognizing complement receptor type 3. Immunology: 57, 239-247 (1986).

Distribution of                       OX-41 and OX-42 antigens (Robinson et al. 1986, modified):

medulla of lymph node:

Red pulp of spleen                   ++                ++

Follicular TBM#                      ++                 +

Splenic marginal zone                 -                 +

IDC** of spleen and lymph node +              ++

Liver:

Kupffer cells                                 +            ++

Brain:

Glial cells                                    ++           Microglia only

Kolmer cells                                  +             ++

Skin:

Langerhans cells                            ++            ++

Dermis                                            +             ++

Rejecting skin grafts                        +             ++

Kidney:

Mesangial                                         +             +

Interstitial                                       - +            <W3/25

Thymus:                                            +             +

Interstitial cells:

of small intestine                                +            +

of testis                                               +            +

of heart                                                 -            +

++ Majority + Some + Few - No macrophages or non-lymphoid cells appeared labelled.

# TBM, Tingible body macrophages ** IDC, Interdigitating cells

Mouse anti-rat monocyte/macrophage/dendritic cell clone ED1

-useful for the detection of isolated dendritic (veiled) cells the blood. The antibody recognizes a single chain glycoprotein of 90-100kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by EDI has many characteristics in common with microsialin in th emouse and CD68 in the human. The antigen is found on 90% of monocytes in the peripheral blood. It is also expressed by 98% of isolated dendritic (veiled) cells.

-ascites, mIgG1, suitable for acetone fixed frozen sections, paraffin sections and FACS, western blottinh

-ref: Immunology 54, 589 (1985),  Immunology 83, 140-147 (1994), J. Neurosci Res 38, 365-375 (1994)

-cat#RDI-T3003X      $500.00/0.25ml (250ug) vial

Monoclonal Antibody ED1

Product Information

Anti Rat Monocytes / Macrophages & Dendritic cells

ED1 is useful for the detection of rat monocytes and macrophages and isolated dendritic (veiled) cells in the blood. The antibody recognises a single chain glycoprotein of 90-100kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 has many characteristics in common with macrosialin in the mouse and CD68 in the human.

Applications: Immunohistology on frozen and paraffin sections, Western blotting and immunoprecipitation of the antigen, FACS (preferably on permeabilized cells).

Lot number:

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Purity / Physical state: Purified IgG / liquid solution

Quantity / Volume: 250ug / 250ml

Buffer: Phosphate buffered saline, pH 7.2

Preservative: 0.1% Sodium azide

Storage: Aliquots of stock solution can be kept frozen at -20°C;  Avoid repeated thawing and refreezing

Stability of stock solution: 1 year at -20°C

Working dilution: 0.5 - 1mg/ml in FACS and immune histology, but optimal concentration should be tested by serial dilution

For laboratory use and research purposes only.

Caution: this product contains sodium azide, a poisonous and hazardous substance!

SELECTED LITERATURE

DIJKSTRA, C.D et al.: The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2 and ED3. Immunology: 54, 589 - 599 (1985).

BEELEN, R.H.J et al.: Monoclonal Antibodies ED1, ED2, and ED3 Against Rat Macrophages: Expression of Recognized Antigens in Different Stages of Differentiation. Transplantation Proceedings: XIX, (3), 3166-3170 (1987).

DAMOISEAUX, J.G.M.C. et al.: Rat macrophage lysosomal mambrane antigen recognised by monoclonal antibody ED1. Immunology 83, 140-147 (1994)

BAUER, J. et al.: Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis. J. Neurosci. Res. 38, 365-375 (1994).

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Rat: monocytes, macrophages, dendritic cells

Other species: unknown

BIOCHEMISTRY

ED 1 recognises a 92kD cytoplasmic protein.

ANTIGEN DISTRIBUTION

The antigen is found on 90% of monocytes in the peripheral blood. It is also expressed by 98% of isolated dendritic (veiled) cels.

-useful for detecting phagocytic tissue macrophages in various organs of the rat (Kupffer cells, peritoneal, splenic red pulp, lymphoid sinus and germinal macrophages), recognizes a surface antigen of 45kda wieught as a single band under reducing conditions. Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kuppfer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of hte pulp and sinus, tingible body macrophages), lung (approx 50% of alveolar macrophages) and connective tissue (histiocytes) are positively stained. All blood cells (excluding stimulated monocytes) as wells as sinus lining cells, cells of the "immature sinus histiocytosis" and "sinus catarrh are negative).

In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoeitic and magacaryocytes are negative. Immature accessory cells such as dendritic or intergitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material.

-purified, mIgG1, suitable for acetone, formaldehyde and parrafin sections (enzyme digestion for 10-15 minutes)

-ref: J. Leukocyte Biology 38, 509 (1985),  J. Leuk Biol:41, 70-77 (1987)

-cat# RDI-T3004X   $565.00/100 ug vial    

Product Information

Rat pan-macrophages

Ki-M2R is an antibody that recognizes typical tissue macrophages and specifically discriminates between monocytes (Ki-M2R-) and macrophages(Ki-M2R+). It stains all resident macrophages including those of bone marrow, lymphatic sinuses, lymphoid follicles, splenic red pulp, and Kupffer cells in the liver. Langerhans cells, interdigitating reticulum cells, and dendritic reticulum cells of lymphoid follicles are not stained. Ki-M2R is a reliable tool for indicating macrophage differentiation in vivo and in vitro.

Applications: Immune histology.

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Purity: Affinity purified IgG from culture supernatant

Quantity: 100ug

Physical state: Freeze-dried

Buffer: Phosphate buffered saline pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Reconstitute in: 0.5ml distilled water (=stock solution)

Storage: Aliquots of stock solution can be kept frozen at -70°C; do not freeze working dilutions!!

Stability of stock solution: 1 year at -70°C

Approximate working dilution: optimal concentration should be tested by serial dilution Immunohistology: 1:200 (cryosections), 1:100 (paraffin sections) Pretreatment of paraffin tissue: enzyme digestion required (10-15min)

For laboratory use and research purposes only!

Caution: this product contains Thimerosal, a hazardous and poisonous substance!

SELECTED LITERATURE

Wacker, H.H. et al.: Ki-M2R a new specific monoclonal antibody, discrimates tissue macrophages from reticulum cells and moncytes in vivo and in vitro. J.Leuk.Biol.: 38, 509 - 520 (1985).

Wacker, H.H. et al.: Kinetics of Kuppfer cells as shown by parabiosis and combined autoradiographic/immunohistochemical analysis. Virch.Arch. B. (Cell Pathol.): 51, 71 - 78 (1986).

Wacker, H.H. et al.: Selective recognition of Rat Follicular Dendritic Cells (Dendritic Reticulum Cells) by a New Monoclonal Antibody KI-M4R In Vitro and In Vivo. J. Leuk. Biol. 41, 70-77 (1987).

BIOLOGICAL CHARACTERISTIC

SSPECIFICITY

Rat: mature macrophages

Other species: unknown

BIOCHEMISTRY

Ki-M2R recognises a surface antigen of 45kD molecular weight as a single band under reducing conditions. The epitope is resistant to different fixations and paraffin processing.

ANTIGEN DISTRIBUTION

Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kuppfer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50% of alveolar macrophages) and connective tissue (histiocytes) are positively stained. Plasma cells (excluding stimulated monocytes) as well as sinus lining cells are negative.

In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoetic cells and megacaryocytes are negative. Immature accessory cells such as dendritic or interdigitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material.

-clone RM1, positive on monocytes and macrophages.It is a member of a monoclonal antibody family which characterizes perionteal macrophages well. It is also a useful detectiom marker of rat interdigitating cells and their in vitro equivalents. As it recognizes a cell surface antigen, it may also be useful for FACS analysis. In combination with other monoclonal antibdoies, clone RM-1 is very suitable for the characterization of macrophage subpopulations in various organs.

-purified, mIgG1, suitable for acetone and frozen cells, paraffin

-ref: J. Histochem. Cytochem. 37, 635 (1989)

-cat# RDI-T3005X     $531.00/100 test vial    

Product Information

anti Rat Macrophages and Interdigitating Cells

RM-1 recognizes a surface antigen of unknown fuction on monocytes, macrophages, interdigitating cells and their in vitro equivalents.

Applications: Immune histology, FACS.

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Quantity: 100ug

Purity: purified IgG from culture supernatant

Physical State: freeze-dried powder

Buffer:: phosphate buffered saline, pH 7.2

Reconstitute: in 0.5 ml distilled water (= stock solution)

Preservative: Thimerosal 0.01 %

Stabilizer: Bovine serum albumin 10 mg/ml

Storage: unreconstituted at 4°C over 1 year  after reconstitution aliquot and freeze at -20°C

Approximate Working Dilution: optimal concentration should be tested by serial dilutions. Histology: 1/100 on cryostat sections

Fixation: 2% paraformaldehyde-lysine / sodium periodate or acetone

For laboratory use and research purposes only!

Caution: this product contains Thimerosal, a poisonous and hazardous substance.

References:

Takeya, M.et al.: Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study. J. Histochem Cytochem: 37: 635-641 (1989)

Kato,T. et al.: Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analysis with immunohistochemistry, immunoelectron microscopy and 3H thymidine autoradiography. Lab Invest: 62: 635-645 (1990)

Izumi,S. et al.: Ontogenic Development of synovial A cells in fetal and neonatal rat knee joints. Cell Tiss Res : 262: 1-8 (1990).

Kasper, M. et al.: Heterogenous Dolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem 95: 1-11 (1993)

BIOLOGICAL CHARACTERISTICS

Specificity:

Rat: monocytes, subpopulation of macrophages, interdigitating cells

Other Species: unknown

Biochemistry:

The antigen is localized on the surface of cells. It is stable to 2% periodate-lysine-paraformaldehyde fixation

Antigen Distribution:

Isolated cells:

Positive on monocytes in peripheral blood

Positive on 20-30% peritoneal cells, increasing to 30-40% after thioglycolate stimulation

Tissue sections:

The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.

(accessory B-cell/antigen trapping macrophage)

-clone KI-M9R ab is one of three accesory B cell markers and is useful for the identification of very distinct macrophage subpopulations in lymphoid organs.

-purified,mIgG1, suitable for acetone fixed ,histology

-cat# RDI-T3008X, 100T  $531.00/vial    

Rat follicular dendritic cells

Ki-M9R detects a cytoplasmic antigen in accessory B-cells which are involved in antigen trapping reactions. The antigen is only expressed in sinus lining cells of lymph nodes, in follicular dendritic cells of germinal centres, and in metallophilic cells of the spleen. Thus it seems that the antigen is associated with antigen processing cells that migrate to germinal centres to develop into follicular dendritic cells.

Application: Immune histology

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Quantity: 500ug

Purity: Affinity purified

Physical state: Lyophilized

Buffer: Phosphate buffered saline pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Reconstitute in: 0.5 ml distilled water (=stock solution)

Storage: Aliquots of stock solution can be kept frozen at -70°C; do not freeze working dilutions

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 5mg/ml (1:200) freshly prepared

Fixation: Acetone

For laboratory use and research purposes only

Caution: this product contains Thimerosal, a poisonous and hazardous substance!

References:

Data obtained in the laboratory of the Institute of Pathology, Kiel, Germany

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Rat: subpopulation of macrophages

Other species: unknown

BIOCHEMISTRY

Ki-M9R detects an epitope of a 49kD cytoplasmic antigen

ANTIGEN DISTRIBUTION

Isolated cells:

Ki-M9R is negative with all peripheral blood cells and cells of the bone marrow or peritoneal cavity.

Tissue sections:

Ki-M9R specifically recognises antigen trapping macrophages of the B-cell system such as sinus lining cells and dendritic reticulum cells. It is also positive on splenic metallophils. Macrophages in T-cell areas are negative with Ki-M9R. None of the macrophages in non-lymphoid organs are detected by Ki-M 9R. No cross-reactivity was found with other cell types at different tissue sites except with hepatic sinusoids. In vivo adminstration of Ki-M9R reveals a direct cytotoxicity to, and the elimation of Ki-M9R positive cells. Animals survive this treatment.

COMMENTS

During the course of an infection the number of antigen trapping cells decreases while the number of antigen presenting cells increases.

-clone ED2 ab is a suitable marker for characterizing various tissue fixed macrophages.

-clone ED2 reacts with a membrane antigen (175, 160 and 95kDa) on resident rat macrophages. Monocytes, dendritic cells (spleen and lymph node), peritoneal granulocytes and other cell types are negative. ED2 discriminates between thymic cortical (ED2+) and medullary (ED2-) macrophages. It is useful to stain synovial lining cells and Kupffer cells in the liver., mIgg1,

-ref: Immunology 54, 589 (1985),  J Histochem 43:313-320 (1995)

-ascites,mIgG1, suitable for acetone fixed (1:500) , paraffin( periodate lysine paraformaldehyde fixation),, histology, Facs (1:10-1:100)

-cat# RDI-T3011X  , 0.25ml/0.25mg    $500.00/vial  

Product Information

Rat synovial lining cells and resident macrophages

ED2 reacts with a membrane antigen (175, 160 and 95kDa) on resident rat macrophages. Monocytes, dendritic cells (spleen and lymph node), peritoneal granulocytes and other cell types are negative. ED2 discriminates between thymic cortical (positive for ED2) and medullary (negative for ED2) macrophages. It is useful to stain synovial lining cells and Kupffer cells in the liver.

Applications: Immune histology, FACS.

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Purity: Purified from tissue culture supernatant

Physical state: Liquid

Quantity, concentration: 250mg, 0.75mg/ml

Preservative: 0.1 % Sodium azide

Storage: Aliquots of stock solution can be kept frozen at -20°C;

Avoid repeated thawing and refreezing

Stability of stock solution: 1 year at -20°C

Approximate working dilution: 1:400 for cryosections

                                                  1:10 - 1:50 for FACS

Fixation: Acetone

This clone has been described reacting with paraffin-embedded material following periodate-lysine-paraformaldehyde fixation (see Ref. 3)

For laboratory use and research purposes only!

Caution: this material contains sodium azide, a hazardous and poisonous substance!

SELECTED LITERATURE

1) Dijkstra, C.D. et al.: The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED 1, ED 2 and ED 3. Immunology: 54, 589 - 599 (1985).

2) Beelen, R.H.J. et al.: Monoclonal Antibodies ED 1, ED 2, and ED 3 Against Rat Macrophages: Expression of Recognized Antigens in Different Stages of Differentiation. Transplantation Proceedings: XIX, (3), 3166-3170 (1987).

3) Whiteland, J.L et al.: Immunohistochemical Detection of T-cell subsets and other leucocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43, 313-320 (1995).

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Rat: Macrophages

Other species: unknown

BIOCHEMISTRY

ED2 detects an epitope of a heterodimer 95kD, 160kD and 175kD membrane antigen. The antigen is sensitive to routine paraffin embedding.

ANTIGEN DISTRIBUTION

Isolated cells:

Monocytes and dendritic cells harvested from spleen and lymph nodes, and granulocytes harvested from the peritoneal cavity are negative with ED 2.

Staining of macrophages by ED1, ED2 and ED3 (Dijkstra et al., 1985, modified):

-clone OX-42, useful for the identification of microglial cells. In combination with clone OX-41 it facilitates a more distinct phenotyping of resident macrophages in certian tissues.

-ascites, mIgG2a, suitable for acetone fixed and flow cytometry

-ref: J. Exp Med 166, 1138 (1987)

-cat# RDI-T3102X    $625.00/1mg

same clone also available in smaller size see rat CD11bc    cat#RDI-RTCD11bc-OX

$438.00/0.5mg or    azide free cat#RDI-RTCD11BC-OXP     $656.00/0.5mg

DATA SHEET: mouse anti-RAT CD11B/C

Catalog#: RDI-RTCD11BC-OX $406.00/vial

Package Size: 500ug purified in 1.0ml (0.5mg/ml) 10mM phosphate buffer pH7.2 with 150mM NaCl and 0.09% sodium azide

Species: mouse IgG2a

CLONE: OX-42

Immunogen: resident peritoneal cells from (PVG.RT1c X PVG.RT1u) and (PVG.RT1c X PVG.RT1a) F1- hyrbid rats.

Activity: reacts with the CR3 complement (C3bi) receptor found in most monocytes, granulocytes, and macrophages. Clone OX-42 inhibits C3bi binding activity. It appears to recognize a common epitope shared by CD11b and CD11c (integrin am and ax subunits). Clone OX-42 antibody inhibits C3Bi binding activity.

Application: -Indirect immunofluorescence cell surface staining.

                     -immunoprecipitation

                     -function blocking

                      -histochemistry (acetone fixed frozen sections, 1-5ug/ml)

Storage: Store at 4 DEG C

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

References: -Tamatani, T. et al, Eur J. Immunol 23:2181-2188

                   -Tamatani, T t al, Eur J. Immunol. 21:627- 633 (1991)

                  -Immunology 57:239-247 (1986)

                 -Am J Pathol 143:410-418

It is the responsibility of the user to comply with all local/state andFederal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

also available:FITC labeled- cat#RDI-RTCD11BC-OXFT $625.00/0.5mg

azide free and sterile filtered cat#RDI-RTCD11BC-OXP $625.00/0.5mg

-useful marker for detection of rat granulocytes in frozen tissue sections and in enzyme treated paraffin embedded tissues. It is also suitable for the isolation of granulocytes from the blood and their precursors from bone marrow cell suspensions by FACS.

-purified, mIgG1, suitable for acetone, frozen and paraffin

sections and FACS.

-ref: Immunobiology 181, 324 (1990)

-cat#RDI-T3105X   $531.00/100 test vial

-cat#RDI-3119X   Biotin labeled $594.00/100ug

Product Information  anti Rat Granulocytes

RK-4 detects all rat granulocytes in frozen, and in paraffin embedded rat tissue sections after enzyme digestion.

Applications: Immune histology, FACS

TECHNICAL CHARACTERISTICS

Species: Mouse

Class/Subclass: IgG1

Quantity: 75ug

Purity: Affinity purified IgG

Physical state: Freeze-dried

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Reconstitute in: 0.5ml distilled water (=stock solution)

Buffer: PBS pH 7.2

Storage: Aliquots of stock solution can be kept frozen at -70°C; do not freeze working dilutions!!

Stability of stock solution: 1 year at -70°C

Fixation: Acetone 5 min 4°C, buffered 4% paraformaldehyde paraffin embedded tissues should be predigested for 10min with Type XIV protease (Sigma: P/4914) Approximate working dilution: Histochemistry: 1/200 freshly prepared on cryosections,  1/20-1/50 on paraffin embedded material   FACS: 1/50-1/100

For laboratory use and research purposes only

Caution: this product contains Thimerosal, a poisonous and hazardous substance.

SELECTED LITERATURE

F. Steinhausen, H. Xu, H.-J. Groene, H.J. Peters: A monoclonal Antibody Selectively Recognizing Rat Granulocytes. Immunobiol: 181: 324 (1990)

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Rat: pan granulocytes

Other species: human negative, other species unknown

BIOCHEMISTRY

The molecular structure of the antigen is not known. It is localized on the cell surface and stable for paraffin embedding.

ANTIGEN DISTRIBUTION

Isolated Cells:

Only a low percentage of cells from peripheral blood stains positive.

Positive on peritoneal cells 18h after thioglycollate injection (95% granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection.

Negative on isolated lymphocytes, monocytes,erythrocytes and platelets.

Tissue staining:

RK-4 reaction pattern:

Tissues tested Results

Bone marrow positive on granulocytes

positive on granulocyte precursors

positive on segmented cells

positive on band cells

positive on metamyelocytes

positive on myelocytes

Lung negative on alveolar macrophages; interstitial tissues are occasionally positive.

Kidney negative; on perivascular granulocytes occasionally positive.

Skin negative; on perivascular granulocytes occasionally positive.

Liver negative; on perivascular granulocytes occasionally positive.

Blood vessel wall negative

broad reactivity:human/baboon, cat, dog, guinea pig, monkey,   rabbit, rat ,swine/pig an dmouse

Catalog#: RDI-MACROPabm-387  $438.00

Package Size: 200ug in 1ml PBS with 0.2% BSa and 0.09% sodium azide

Clone: MAC387

Ig Isotype: mIgG1

Purification: Protein G

Immunogen: Affinity purified monocyte membrane preparation

Reactivity: Reacts with the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD Beta chain. -reacts with neutrophils, monocytes, macrophages and squamous mucosal epithelia

Species: human, cat, dog, guinea pig, horse, monkey, rabbit, rat , pig and mouse

Use: 1-5ug/ml :histochemistry (frozen and paraffin sections)-For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37 o C

Staining: cytoplasmic

Positive control: tonsil, lympoh node, spleen

ref: -J. Histochem Cytochem 35:1217-1226

      -J. Clin. Path 41:963-970

      -Histopathol 21:191-196

     -Andersen CB; et al. Apmis, 1994, 102(1):23-37.

     -Coleman N; Stanley MA. et al. Human Pathology, 1994, 25(1):73-9.

Storage: Store at 4 DEG C

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

-$312.00/vial 0.5ml

-cross reacts with rat and mouse pancreatic elastase. Does not cross react with porcine or human neutrophil elastase.

-$150.00/2ml  

-antisera strongly agglutinates rat PMNs but not lymphocytes at

dilutions of 1:20 -1:200. Cytotoxic on rat PMNS at 1:20 (>80%) but <15%

cytotoxic on rat thymocytes or splenocytes.

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