rev:  April 28, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Monoclonal anti-RecA  (see 4 clones below)
Mouse anti-RecA Monoclonal ab

cat# RDI-RECAabm-193   $500.00/vial 100ug

isotype: mIgG2b

Clone: ARM193

Antigen: purified RecA protein from E. Coli

Hybridoma: myeloma (P3X.63-Ag8.653) X Mouse(Balb/c) spleen cells

package: 100 micrograms IgG fraction purified from ascites by ammonium sulfate precipitation and Protein A purified, lyophilized from PBS with 10mg/ml sucrose and 1mg/ml sodium azide

Reconstitution: dissolve in 100ul DI water, Allow to set at least 1 hour at 4 DEG C, Mix well.

Use: Suitable for western blot:approx 1ug/ml with enhanced chemiluminescence detection -band at approx 40kDa using positive control of E. Coli whole extract -inhibition:concentration equal to RecA protein

Reactivity: -reacts with RecA of E.Coli. It recognizes amino acid residues Phe260-Glu347 of proteolytic fragments of RecA polypeptide. -This clone Mab inhibits shDNA dependent ATPase activity of Rec A protein and does not inhibit D- loop formation activity and ssDNA dependent ATPase activity of RecA protein.

Storage: Store 4 DEG C (before opening), -20 DEG C after reconstitution. Recommend aliquoting. Avoid frequent freeze and thaw cycles.

Ref: -Shibata, T. et al. (1983):Methods Enzymol., 100,197-209

        -Makino,O et al.(9185):J. Biol Chem., 265,8948 -8956

        -Ikeda,M.et al.(1990):J. Biol. Chem., 265,8948- 8956

       -Shibata,T. et al.,(1991):Biochemin., 73,209-217

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state andFederal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner see other clones ARM321, ARM191, ARM414


Mouse anti-RecA Monoclonal ab

cat# RDI-RECAabm-321   $500.00/vial 100ug

isotype: mIgG1

Clone: ARM321

Antigen: purified RecA protein from E. Coli

Hybridoma: myeloma (P3X.63-Ag8.653) X Mouse(Balb/c) spleen cells

package: 100 micrograms IgG fraction purified from ascites by ammonium sulfate precipitation and Protein A purified, lyophilized from PBS with 10mg/ml sucrose and 1mg/ml sodium azide

Reconstitution: dissolve in 100ul DI water, Allow to set at least 1 hour at 4 DEG C, Mix well.

Use: Suitable for western blot:approx 1ug/ml with enhanced chemiluminescence detection -band at approx 40kDa using positive control of E. Coli whole extract -inhibition:concentration equal to RecA protein

Reactivity: -reacts with RecA of E.Coli. It recognizes amino acid residues Thr89-Glu127 of proteolytic fragments of RecA polypeptide. -This clone Mab inhibits D loop formation activity, sHDNA dependent ATPase activity and ssDNA dependent ATPase activity of RecA protein.

Storage: Store 4 DEG C (before opening), -20 DEG C after reconstitution. Recommend aliquoting. Avoid frequent freeze and thaw cycles.

Ref: -Shibata, T. et al. (1983):Methods Enzymol., 100,197-209

       -Makino,O et al.(9185):J. Biol Chem., 265,8948 -8956

      -Ikeda,M.et al.(1990):J. Biol. Chem., 265,8948- 8956

      -Shibata,T. et al.,(1991):Biochemin., 73,209-217

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner see other clones ARM193, ARM191, ARM414


Mouse anti-RecA Monoclonal ab

cat# RDI-RECAabm-191      $500.00/vial 100ug

        RDI-RECAabm-414

isotype: mIgG1       mIgG2b

Clone: ARM191     ARM414

Antigen: purified RecA protein from E. Coli

Hybridoma: myeloma (P3X.63-Ag8.653) X Mouse(Balb/c) spleen cells

package: 100 micrograms IgG fraction purified from ascites by ammonium sulfate precipitation and Protein A purified, in 100ul (1mg/ml) PBS with 0.1% sodium azide

Use: Suitable for western blot:approx 1ug/ml with enhanced chemiluminescence detection -band at approx 40kDa using positive control of E. Coli whole extract -inhibition:concentration equal to RecA protein

Reactivity: -reacts with RecA of E.Coli. It recognizes amino acid residues Phe260-Glu347(clone ARM191) or Glu233- Lys256(clone ARM414) of proteolytic fragments of RecA polypeptide. -This clone Mab inhibits D loop formation activity, sHDNA dependent ATPase activity and ssDNA dependent ATPase activity activity of RecA protein.

Storage: Store -20 DEGC (avoid frequent freeze thaw cycles)

Ref: -Shibata, T. et al. (1983):Methods Enzymol., 100,197-209

       -Makino,O et al.(9185):J. Biol Chem., 265,8948 -8956

       -Ikeda,M.et al.(1990):J. Biol. Chem., 265,8948- 8956

      -Shibata,T. et al.,(1991):Biochemin., 73,209-217

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner see other clones ARM193, ARM191, ARM414


RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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