rev:  January 27, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DATA SHEET: GOAT anti-Pyruvate kinase

Catalog#: RDI-PYRUKINabG     $312.00/vial   

Also available HRP conjugated: cat#RDI-PYRUKIN-HRP   $375.00/20mg vial

                      Biotin conjugated  cat#RDI-PYRUKIN-BT     $375.00/10mg vial

Size: 50 mg (lyophilized)

Antibody Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: Pyruvate Kinase (rabbit muscle).

Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Reactivity: This polyclonal antibody reacts with the tetrameric enzyme pyruvate kinase (PK) from rabbit muscle showing a 237 kDa band (tetramer) under non-reducing conditions of SDS-PAGE and a 57 kDa band when reduced. PK is a key enzyme in glycogen metabolism. PK from rabbit muscle has been most widely studied. The antibody may facilitate the analysis of this enzyme in various assays. Reactivity with the enzyme isolated from other sources is unknown, but likely due to the nature of the polyclonal antibody, ELISA, immunoblotting, immunoprecipitation.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

Purity and Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Rabbit Serum as well as purified and partially purified pyruvate kinase(rabbit muscle ). Cross reactivity against pyruvate kinase from other tissues and species may occur but have not been specifically determined.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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