rev:    February 13, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Prostate Specific Antigen (PSA)  (see below for total and free PSA abs)  see also human PSA antigens

-PSA Matched Pairs see  below

Elisa Kit for research use only  at#RDI-PSA-KT  $375.00/kit,   $312.00/kit 4+ 96 well Elisa


Mouse anti-Prostate Specific Antigen   (Cocktail) for WB & histo

cat# RDI-PSA85abm             $375.00/100ug

       RDI-PSA85abm-1          $500.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified human PSA

Isotype: mIgG1 (both)

Clones: ER-PR8 & PA05

Reactivity: human(others not tested)

Uses: -Western blot: 1ug/ml 2 hr rt

-histochemistry (frozen and paraffin sections) approx 1-2ug/ml 30-60 minutes RT

-for formalin fixed sections, requires pretreatment in boiling 10mM citrate bufer pH6.0 for 10-20 minutes followed by cooling at RT for 20 min.

Positive Contrl: prostate or prostate carcinoma

Localization: Cytoplasmic

Background: Recognizes a single protein of 33-34kDa, identified as PSA. This "cocktail" is composed of two clones those epitopes are not precisely mapped, but experimental data suggets that they are different. This cocktail is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate pithelium, and abundant in seninal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver derived serine protease inhibitor, alpha-1- antichymotrypsin. A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Storage: 2-8 deg c


For Research Use Only


DATA SHEET :Mouse anti-Human Prostate Specific Antigen (PSA)   (frozen & Paraffin Sections)

cat# RDI-PSAabm-A4  $375.00/vial

Preparation: Lyophilized antibody purified by Protein G affinity chromatography diluted in 1% w/v BSA in PBS with 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.

Clone: PSA 28/A4

Antigen: seminal fluid preparation

Isotype: mIgG1k

Specificity: Human prostate specific antigen (this antibody reacts with prostatic secretory and ductal epithelium in both normal and neoplastic tissue and can be useful in the identification of metastitic carninomas of prostatic origin)

USE: Effective on paraffin wax embedded tissue and on frozen sections.

Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 60 Minutes incubation at 25'C. Standard ABC technique.

Positive controls: normal prostate gland or known prostate carcinoma

Staining pattern: cytoplasmic

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily. Stricly for in vitro research use only-Not for use in or on humans or animals-not for use in diagnostics


Monoclonal mouse anti-prostate specific antigen (PSA)

Catalogue #   RDI-TRK4P33-xxx               xxx=clone#

$375.00/1mg   $344.00/mg 2-9     $312.00/mg 10+

Clone: 2H9, 5A6, 8A6, PS1, PS2      New clones - PS6, 5G6

These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with human PSA antigen purified from semenal plasma.

Reactivity: Specificity of the clone is described in the table  (see below)

Application: Detection of PSA antigen.

Recommended pairs for detection of total PSA : 5A6-2H9, PS2 - PS6, 5G6 - 5A6.

Best pair : 5G6 - 5A6

Recommended pairs for detection of free PSA : 5A6-8A6, 2H9-8A6, PS2-PS1, PS1-PS2, 8A6 - 5A6

Best pair : 8A6 - 5A6

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7,4, containing 0,1% of sodium azide as preservative. Store at +4C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Mab Isotype Specificity in ELISA Cross-reactivity with human

kallikrein 2

5G6 IgG1 Total PSA, epitope 4 0.00
PS6 IgG1 Total PSA, epitope 4 0.00
2H9 IgG2a equimolar total PSA, epitope 3. 50%
PS2 IgG1 equimolar total PSA, epitope 3 100%
5A6 IgG2a equimolar total PSA epitope 6 0.00
8A6 IgG2a free PSA epitope 1 0.00
PS1 IgG1 free PSA, epitope 1 0.00

For Research Use Only


Mouse anti-Human PSA MONOCLONAL

cat# RDI-PSAabm-280 $375.00/1mg

Clone# 280

Lot# see batch sheet

Use: Coating antibody for solid phase

Class: mouse IgG

Antigen: human seminal fluid

Presentation: Chromatographically purified immunoglobulin fraction in 0.05M PBS, pH 7.4 containing 0.1% NaN3.

Volume: see batch sheet

Protein conc: see batch sheet

Affinity: 8 X 109

Storage: Store at 4-8 DEG C Upon Receipt. Avoid Frequent freeze thaw cycles.

For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.

Price: Price: $375.00/mg 1-9mg     $312.00/mg 10mg +     Bulk on request


Mouse anti-Human PSA MONOCLONAL (Total-detecting ab)

cat: RDI-PSAabm-281        Price: Price: Price: $375.00/mg 1-9mg  $344.00/mg 10mg  +

Bulk on request

Clone# 281

Lot# see batch sheet

Use: Detecting antibody (enzyme or 125I labeled )

Class: mouse IgG

Antigen: human seminal fluid

Presentation: Chromatographically purified immunoglobulin fraction in 0.05M PBS, pH 7.4 containing 0.1% NaN3.

Volume: see batch sheet

Protein conc: see batch sheet

Affinity: 6 X 10 E09

Storage: Store at 4 -8 DEG C Upon Receipt.

also: directly conjugated anti-PSA:HRP moncolonal clone 281

cat#RDI-PSAabm-HRP     $625.00/milligram

For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.


Mouse anti-Human FREE PSA MONOCLONAL


cat: RDI-PSAFabm-65      Price: $500.00/mg 1-5mg       $438.00/mg 6-20mg


Clone# PSA-F65

Lot# see batch sheet

Use: Coating antibody (pair with clone 66 )

-for use in EIA or IRMA assays


Class: mouse IgG1

Antigen: purified human Free PSA

Presentation: Chromatographically purified immunoglobulin fraction in PBS, pH 7.5 containing 0.1% NaN3.

Volume: see batch sheet

Protein conc: see batch sheet

Affinity: approx 2 X 10 E10


Specificity: Free PSA 100%    less than 0.1% with AFP, CEA, Ca 19-9, CA125, PAP, albumin, human IgG)


Storage: Store at 4 -8 DEG C Upon Receipt.

For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.


Mouse anti-Human PSA MONOCLONAL   (Detecting clone in FREE PSA assay)


cat: RDI-PSAabm-66        Price: $500.00/mg 1-5mg       $438.00/mg 6-20mg

Clone# PSA-66

Lot# see batch sheet

Use: Detecting antibody (pair with free PSA clone 65)

-for use in EIA or IRMA assays

Class: mouse IgG1

Antigen: purified human PSA

Presentation: Chromatographically purified immunoglobulin fraction in PBS, pH 7.5 containing 0.1% NaN3.

Volume: see batch sheet

Protein conc: see batch sheet

Affinity: approx 7 X 109

Specificity: PSA 100% less than 0.1% with AFP, CEA, CA 19-9, CA125, PAP, albumin, human IgG)

Storage: Store at 4 -8 DEG C Upon Receipt.


For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.

Bulk quotes on Request



RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222 or

          (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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