rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Prostate Specific Antigen (PSA) (see below for total and free PSA abs) see also human PSA antigens
-PSA Matched Pairs see below
Elisa Kit for research use only at#RDI-PSA-KT, new cat#: 55R-PSA-KT $412.50/kit
Mouse anti-Prostate Specific Antigen (Cocktail) for WB & histo
cat# RDI-PSA85abm, new cat#: 10R-P142d $484.00/100ug
RDI-PSA85abm-1 $500.00/200ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Immunogen: purified human PSA
Isotype: mIgG1 (both)
Clones: ER-PR8 & PA05
Reactivity: human(others not tested)
Uses: -Western blot: 1ug/ml 2 hr rt
-histochemistry (frozen and paraffin sections) approx 1-2ug/ml 30-60 minutes RT
-for formalin fixed sections, requires pretreatment in boiling 10mM citrate
bufer pH6.0 for 10-20 minutes followed by cooling at RT for 20 min.
Positive Contrl: prostate or prostate carcinoma
Localization: Cytoplasmic
Background: Recognizes a single protein of 33-34kDa, identified as PSA. This
"cocktail" is composed of two clones those epitopes are not precisely
mapped, but experimental data suggets that they are different. This cocktail is
highly specific to PSA and stains prostatic secretory and ductal epithelium in
both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease
(kallikrein family) exclusively produced by the prostate pithelium, and
abundant in seninal fluid. PSA can be detected in the sera of patients with
prostatic carcinoma. It is predominantly complexed to a liver derived serine
protease inhibitor, alpha-1- antichymotrypsin. A higher proportion of serum PSA
is complexed to ACT in prostate cancer than in benign prostate hyperplasia.
Storage: 2-8 deg c
For Research Use Only
DATA SHEET :Mouse anti-Human Prostate Specific Antigen (PSA) (frozen & Paraffin Sections)
cat# RDI-PSAabm-A4 $375.00/vial
Preparation: Lyophilized antibody purified by Protein G affinity chromatography
diluted in 1% w/v BSA in PBS with 15mM sodium azide. Reconstitute with 1ml of
sterile distilled water.
Clone: PSA 28/A4
Antigen: seminal fluid preparation
Isotype: mIgG1k
Specificity: Human prostate specific antigen (this antibody reacts with
prostatic secretory and ductal epithelium in both normal and neoplastic tissue
and can be useful in the identification of metastitic carninomas of prostatic
origin)
USE: Effective on paraffin wax embedded tissue and on frozen sections.
Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 60
Minutes incubation at 25'C. Standard ABC technique.
Positive controls: normal prostate gland or known prostate carcinoma
Staining pattern: cytoplasmic
Storage and stability: Store unopened lyophilized antibody at 4'C. Under these
conditions, there is no significant loss in product performance up to the
expiry date indicated on the vial label. The reconstituted antibody is stable
for at least two months when stored at 4'C. For long term storage, aliquot in
non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh
working dilutions daily. Stricly for in vitro research use only-Not for use in
or on humans or animals-not for use in diagnostics
Monoclonal mouse anti-prostate specific antigen (PSA)
Catalogue # RDI-TRK4P33-xxx xxx=clone#
$375.00/1mg $344.00/mg 2-9 $312.00/mg 10+
Clone: 2H9, 5A6, 8A6, PS1, PS2 New clones - PS6, 5G6
These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with human PSA antigen purified from semenal plasma.
Reactivity: Specificity of the clone is described in the table (see below)
Application: Detection of PSA antigen.
Recommended pairs for detection of total PSA : 5A6-2H9, PS2 - PS6, 5G6 - 5A6.
Best pair : 5G6 - 5A6
Recommended pairs for detection of free PSA : 5A6-8A6, 2H9-8A6, PS2-PS1, PS1-PS2, 8A6 - 5A6
Best pair : 8A6 - 5A6
Purification: Purified by chromatography on protein A Sepharose. Purity is
tested by electrophoresis.
Presentation: Each vial contains antibodies in PBS, pH 7,4, containing 0,1% of
sodium azide as preservative. Store at +4C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
|
Mab |
Isotype |
Specificity in ELISA |
Cross-reactivity with human kallikrein 2 |
|
5G6 |
IgG1 |
Total PSA, epitope 4 |
0.00 |
|
PS6 |
IgG1 |
Total PSA, epitope 4 |
0.00 |
|
2H9 |
IgG2a |
equimolar total PSA, epitope 3. |
50% |
|
PS2 |
IgG1 |
equimolar total PSA, epitope 3 |
100% |
|
5A6 |
IgG2a |
equimolar total PSA epitope 6 |
0.00 |
|
8A6 |
IgG2a |
free PSA epitope 1 |
0.00 |
|
PS1 |
IgG1 |
free PSA, epitope 1 |
0.00 |
|
|
|
|
|
For Research Use Only
Mouse anti-Human PSA MONOCLONAL
cat# RDI-PSAabm-280, new cat#: 10R-P142e $412.50/1mg
Clone# 280
Use: Coating antibody for solid phase
Class: mouse IgG
Antigen: human seminal fluid
Presentation: Chromatographically purified immunoglobulin fraction in 0.05M PBS, pH 7.4 containing 0.1% NaN3.
Volume: see batch sheet
Protein conc: see batch sheet
Affinity: 8 X 109
Storage: Store at 4-8 DEG C Upon Receipt. Avoid Frequent freeze thaw cycles.
For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.
Price: Price: $375.00/mg 1-9mg $312.00/mg 10mg + Bulk on request
Mouse anti-Human PSA MONOCLONAL (Total-detecting ab)
cat: RDI-PSAabm-281 Price: Price: Price: $375.00/mg 1-9mg $344.00/mg 10mg +
Bulk on request
Clone# 281
Use: Detecting antibody (enzyme or 125I labeled )
Class: mouse IgG
Antigen: human seminal fluid
Presentation: Chromatographically purified immunoglobulin fraction in 0.05M PBS, pH 7.4 containing 0.1% NaN3.
Volume: see batch sheet
Protein conc: see batch sheet
Affinity: 6 X 10 E09
Storage: Store at 4 -8 DEG C Upon Receipt.
also: directly conjugated anti-PSA:HRP moncolonal clone 281
cat#RDI-PSAabm-HRP $625.00/milligram
For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.
Mouse anti-Human FREE PSA MONOCLONAL
cat: RDI-PSAFabm-65, new cat#: 10R-P143a Price: $407.00/1mg
Clone# PSA-F65
Use: Coating antibody (pair with clone 66 )
-for use in EIA or IRMA assays
Class: mouse IgG1
Antigen: purified human Free PSA
Presentation: Chromatographically purified immunoglobulin fraction in PBS, pH 7.5 containing 0.1% NaN3.
Volume: see batch sheet
Protein conc: see batch sheet
Affinity: approx 2 X 10 E10
Specificity: Free PSA 100% less than 0.1% with AFP, CEA, Ca 19-9,
CA125, PAP, albumin, human IgG)
Storage: Store at 4 -8 DEG C Upon Receipt.
For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.
Mouse anti-Human PSA MONOCLONAL (Detecting clone in FREE PSA assay)
cat: RDI-PSAabm-66, new cat#: 10R-P142c Price: $550.00/1mg
Clone# PSA-66
Use: Detecting antibody (pair with free PSA clone 65)
-for use in EIA or IRMA assays
Class: mouse IgG1
Antigen: purified human PSA
Presentation: Chromatographically purified immunoglobulin fraction in PBS, pH 7.5 containing 0.1% NaN3.
Volume: see batch sheet
Protein conc: see batch sheet
Affinity: approx 7 X 109
Specificity: PSA 100% less than 0.1% with AFP, CEA, CA 19-9, CA125, PAP, albumin, human IgG)
Storage: Store at 4 -8 DEG C Upon Receipt.
For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.
Bulk quotes on Request
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222 or
(978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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