rev:    January 11, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-26S & 20S Alpha Type 1 Proteasome subunit polyclonals

see also monoclonal antibodies to Proteasome (   P27 subunit,      P32 subunit,    P52 subunit)


Background: Proteolytic degradation is critical to the maintenance of   appropriate levels of short-lived and regulatory proteins as   important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome and Liddle syndrome (endothelial sodium channels,).continued

Gen ref: Voges, D. et al. The 26S proteasome: a molecular machine designed for controlled proteolysis. Annu. Rev. Biochem. 68:1015-1068, 1999.

Schwartz, A.L. and Ciechanover, A. The ubiquitin-proteasome pathway and pathogenesis of human diseases. Annu.

Rev. Med. 50:57-74, 1999.


Rabbit anti-Proteasome 19S Subunit S1 (Human)

cat#RDI-PROT19S1abr $469.00/100ul

-immunogen: synthetic peptide aa929-953 of human proteasome regulatory subunit 1

-suitable for western blot to detect the 105kDa S1 subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 19S Subunit S10B (Human)

cat#RDI-PROT1910abr $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S10B

-suitable for western blot and immunoprecipitation procedures to detect the 42kDa S10 subunit in HeLa cell lystae and partially purified 26S proteasome. To a lesser extent an 30kda protein is also detected which might correspond to a subunit degradation.


Rabbit anti-Proteasome 19S Subunit 10B (Human/MS/RT/DOG/Ham)

cat#RDI-PROT1910Mabr $469.00/100ul (Human/MS/RT/DOG/Ham)

-immunogen: synthetic peptide aa278-294 of human proteasome regulatory subunit 10B

-suitable for western blot to detect the 42, 46, 48 and 52kDa S8 subunit in HeLa cell lystae and partially purified 26S proteasome. To a lesser extent an 30kda protein is also detected which might correspond to a subunit degradation.-


Rabbit anti-Proteasome 19S Subunit S2

cat#RDI-PROT19S2abr $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S2

-suitable for western blot and immunoprecipitation procedures to detect the 100Da S2 subunit in HeLa cell lystae and partially purified 26S proteasome. To a lesser extent an 50kda protein is also detected which might correspond to a subunit degradation.


Rabbit anti-Proteasome 19S Subunit S3 (Human)

cat#RDI-PROT19S3abr  $469.00/100ul

-immunogen: synthetic peptide aa513-534 of human proteasome regulatory subunit S3

-suitable for western blot to detect the 60kDa S3 subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 19S Subunit S4

cat#RDI-PROT19S4abr  $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S4

-suitable for western blot and immunoprecipitation procedures to detect the 56Da S4 subunit in HeLa cell lysate and partially purified 26S proteasome. To a lesser extent an 35kda protein is also detected which might correspond to a subunit degradation.


Rabbit anti-Proteasome 19S Subunit S5A

cat#RDI-PROT26S5abr   $469.00/200ul

-immunogen: recombinant human proteasome 26S subunit S5A

-suitable for western blot and immunoprecipitation procedures to detect the 50Da S5A subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 19S Subunit S5B

cat#RDI-PROT26S5Babr   $469.00/100ug

--immunogen: synthetic peptide aa485-504 of human proteasome regulatory subunit S5b

-suitable for western blot to detect the 55Da S5b subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 26S Subunit S6

cat#RDI-PROT19S6abr   $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S6

-suitable for western blot and immunoprecipitation procedures to detect the 48Da S6 subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 26S Subunit S6'

cat#RDI-PROT19S61abr $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S6'

-suitable for western blot and immunoprecipitation procedures to detect the 48Da S6' subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 26S Subunit S7

cat#RDI-PROT19S7abr  $470.00/100ul

-immunogen: recombinant human proteasome 26S subunit S7

-suitable for western blot and immunoprecipitation procedures to detect the 47kDa S7 subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 26S Subunit S8

cat#RDI-PROT19S8abr   $469.00/100ul

-immunogen: recombinant human proteasome 26S subunit S8

-suitable for western blot and immunoprecipitation procedures to detect the 45Da S8 subunit in HeLa cell lysate and partially purified 26S proteasome.


Rabbit anti-Proteasome 20S  C2 Subunit

cat#RDI-PROT20A1abr  $469.00/100ug

-immunogen: peptide corresponding to amino acid residues 249-263 C P(249) A D E P A E K A D E P M E H(263) from the C terminus of human proteasome C2 subunit subunit. This sequence is 93% conserved in the rat and chicken and 87% conserved in the mouse protein.

-suitable for western blot to detect the 29kDa 20S C2 subunit protein in total protein extracts from rat brain, At20, Chinese hamster ovary, HeLa, HepG2, Hepa1 and canine kidney cells.


Rabbit anti-Proteasome 20S LMP2

cat#RDI-PROT20L2abr  $470.00/100ug

-immunogen: peptide corresponding to amino acid residues 207-220 from human and mouse proteasome 20S LMP2. T

-suitable for western blot to detect the approx 23kDa 20S LMP2 from mouse embryo fibroblast. Also recognizes an approx 24kda band which may represent the LMP2 precursor.

-blocking peptide available cat#RDI-PRO20L2-CPX $188.00


Rabbit anti-Proteasome 20S LMP7

cat#RDI-PROT20L7abr  $469.00/100ug

-immunogen: peptide corresponding to amino acid residues 259-274 from human proteasome 20S LMP7. The sequence is identical in human, mouse, rat and sheep.

-suitable for western blot to detect the approx 20kDa 20S LMP7 from HELA cell lysate.

-blocking peptide available cat#RDI-PRO20L7-CPX $188.00


Rabbit anti-Proteasome 20S X

cat#RDI-PROT20SXabr  $470.00/100ug

-immunogen: peptide corresponding to amino acid residues 243-259 from mouse proteasome 20S X. The sequence is 94% conserved in human and rat.

-suitable for western blot to detect the approx 23kDa 20S X

-blocking peptide available cat#RDI-PRO20SX-CPX $188.00


Rabbit anti-Proteasome 20S Y

cat#RDI-PROT20SYabr  $469.00/100ug

-immunogen: peptide corresponding to amino acid residues 41-58 from human proteasome 20S Y. The sequence is 100% conserved in mouse, human ,rat and zebrafish.

-suitable for western blot to detect the approx 25kDa 20S Y

-blocking peptide available cat#RDI-PRO20SY-CPX $188.00


Rabbit anti-Proteasome 20S Z

cat#RDI-PROT20SZabr  $470.00/100ug

-immunogen: peptide corresponding to amino acid residues 263-277 from human proteasome 20S Z. The sequence is 100% conserved in mouse and 97% in rat.

-suitable for western blot to detect the approx 29kDa 20S Z

-blocking peptide available cat#RDI-PRO20SZ-CPX $188.00


Rabbit anti-Proteasome Activator PA28/11S Alpha Subunit

cat#RDI-PROT26ACTabr  $469.00/100ul

-immunogen: peptide corresponding to amino acid residues 5-22 of  human PA28. This sequence differs from the mouse and rat PA28 alpha protein by a single amino acid residue.

-suitable for western blot to detect the 28kDa PA28 alpha subunit in total protein extracts from rat brain, AtT20, chinese hamster ovary, HeLa and canine kidney cells.


Rabbit anti-Proteasome Activator PA28/11S REG GAMMA Subunit

cat#RDI-PROT26ACGabr  $469.00/100ul

-immunogen: peptide corresponding to amino acid residues 1-17 of  mouse and human PA28 gamma.

-suitable for western blot to detect recombinant human 28kda PA28 gamma.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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