rev:   January 29, 2004

HOME (index page)

Return  (alphabetical antibody list)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PROHIBITIN (1 polyclonal and 1 monoclonal available)

-see also new anti-Golgi complex and anti-mitochondria ab)


Rabbit anti-Prohibitin  (Mitochondria Marker)

cat# RDI-PROHIBITabr      $440.00/0.5mg

       RDI-PROHIBITabr-1    $750.00/1mg

Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 1mg/ml

Immunogen: purified recombinant rat prohibitin

Reactivity: human, pig, rat, mouse, chicken

Uses: -Western blot: 5ug/ml for 2 hrs RT

         -immunoprecipitation:10-15ul/mg protein

        -histochemistry (frozen and paraffin sections) -for paraffin sections, requires boiling tissue sections in 10mM cirate buffer pH6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. -approx ab concentration 10-20ug/ml for 1 hr RT

Positive Ctrl: kidney, liver or heart or cell lystates of A427, MAD109.

Localization: Cytoplasmic (mitochondria)

Background: Recognizes a protein of 30kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Prohibitin appears to be a very reliable marker of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor supression.

Storage: 2-8 deg c

general ref: FEBS lett:1995 358:273-277

Exp Gerontol. 1995, 30(2):99-124


Mouse anti-Prohibitin (Mitochondria Marker)

cat# RDI-PROHIBITabm          $440.00/100ug

       RDI-PROHIBITabm-1       $750.00/200ug

       RDI-PROHIBITabm-BT     $535.00/100ug Biotin conjugated

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified recombinant rat prohibitin

Isotype: mIgG1

Clone: II-14-10

Reactivity: human, rat, mouse (others not tested)

Uses: -Western blot: 1ug/ml

          -histochemistry (frozen and paraffin sections)   approx 1:50-1:100 60 minutes RT

Positive Control: kidney, liver or heart

Localization: Cytoplasmic (mitochondria)

Background: Recognizes a protein of 30kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Prohibitin appears to be a very reliable marker of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor supression.

Storage: 2-8 deg c

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (anti-enzyme antibodies..index)

Return  (alphabetical antibody list)

Ordering terms