rev: December 28, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Cat#RDI-PROGPN2 $470.00
Category Guinea pig polyclonal (GP-N2)
Purification / Form Whole antiserum
Immunogen Synthetic peptide (intracellular domain, aa1243-1256)
Description/Specificity The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Antigen recognized in Human, mouse
species (tested so far)
Reactivities on Cultured Cell Lines (tested so far) PCL (podocyte cell line), M-1 (cortical collecting duct cells)
Application
o Immunoblotting (Western blot)
o Immunofluorescence microscopy on frozen tissue
o Immunohistochemistry on paraffin sections, only when using the microwave method
Working Dilution 1:50 for immunohistochemistry
1:500 for immunoblotting
Incubation Time 1 h at room temperature or over night at 2-8°C
Storage At 2-8°C
Quantity 100 µL; contains 0.09% sodium azide
References
Kestila M, Lenkkeri U, Mannikko M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K: Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome. Mol Cell 1, 575-582 (1998).
Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K: Nephrin is specifically located at the slit diaphragm of glomerular podocytes.Proc Natl Acad Sci USA 96, 7962-7967 (1999).
Holthofer H, Ahola H, Solin ML, Wang S, Palmen T, Luimula P, Miettinen A, Kerjaschki D. Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney. Am J Pathol 155, 1681-1687 (1999).
Putaala H, Soininen R, Kilpelainen P, Wartiovaara J, Tryggvason K: The murine nephrin gene is specifically expressed in kidney, brain and pancreas: inactivation of the gene leads to massive proteinuria and neonatal death. Hum Mol Genet 10, 1-8 (2001).
Huh W, Kim DJ, Kim MK, Kim YG, Oh HY, Ruotsalainen V, Tryggvason K: Expression of nephrin in acquired human glomerular disease. Nephrol Dial Transplant 17, 478-484 (2002).
Aaltonen P, Luimula P, Astrom E, Palmen T, Gronholm T, Palojoki E, Jaakkola I, Ahola H, Tikkanen I, Holthofer H: Changes in the expression of nephrin gene and protein in experimental diabetic nephropathy. Lab Invest 81, 1185-1190 (2001).
For Reseach Use Only
Cat#RDI-PROGPN1 $470.00
Category Guinea pig polyclonal (GP-N1)
Purification / Form Whole antiserum
Immunogen Synthetic peptide (extracellular fibronectin domain, aa974-987)
Description/Specificity The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Antigen recognized in species Mouse
(tested so far)
Reactivities on Cultured Cell PCL (podocyte cell line), M-1 (cortical collecting duct cells)
Lines (tested so far)
Application
o Immunoblotting (Western blot)
o Immunohistochemistry of frozen tissue
o Immunocytochemistry (immunofluorescence)
Working Dilution 1:50 for immunohistochemistry
1:500 for immunoblotting
Incubation Time 1 h at room temperature or over night at 2-8°C
Storage At 2-8°C
Quantity 100µL; contains 0.09% sodium azide
References
Kestila M, Lenkkeri U, Mannikko M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K: Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome. Mol Cell. 4, 575-582 (1998).
Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K: Nephrin is specifically located at the slit diaphragm of glomerular podocytes. Proc Natl Acad Sci USA. 96, 7962-7967 (1999).
Holthofer H, Ahola H, Solin ML, Wang S, Palmen T, Luimula P, Miettinen A, Kerjaschki D: Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney. Am J Pathol 155, 1681-1687 (1999).
Putaala H, Soininen R, Kilpelainen P, Wartiovaara J, Tryggvason K: The murine nephrin gene is specifically expressed in kidney, brain and pancreas: inactivation of the gene leads to massive proteinuria and neonatal death. Hum Mol Genet 10, 1-8 (2001).
Huh W, Kim DJ, Kim MK, Kim YG, Oh HY, Ruotsalainen V, Tryggvason K: Expression of nephrin in acquired human glomerular disease. Nephrol Dial Transplant 17, 478-484 (2002).
Aaltonen P, Luimula P, Astrom E, Palmen T, Gronholm T, Palojoki E, Jaakkola I, Ahola H, Tikkanen I, Holthofer H: Changes in the expression of nephrin gene and protein in experimental diabetic nephropathy. Lab Invest 81, 1185-1190 (2001).
For Research Use Only also see cat#RDI-PROGPN2
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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