rev:   November 19, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Progesterone  ( see monoclonals and polyclonals-also inquire for iodination derivatives, Biotin/Hrp and alakaline phos conjugates of Progesterone-other custom derivatives prepared upon request)
Monoclonal mouse anti-progesterone

$375.00/mg   $344/mg 2-9   $281.00/mg 10+

Catalogue # RDI-TRK2P2-XM207 (clone XM207)

                 #RDI-TRK2P2-HPR02 (clone HPRO-2)

Clone: XM207 or HPRO-2 :These clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunised with conjugate of purified progesterone with BSA.

Specificity: Progesterone, Ka = 3.0*109 M-1

Reactivity:            MoAb XM207 reacts with progesterone in ELISA.

Mouse isotype:     IgG2b

Application:         Detection of progesterone.

Purification:         Purified by ion exchange chromatography. Purity is tested by electrophoresis.

Presentation:        Each vial contains antibodies in 0,15 M NaCl, 10 mM Tris, pH 7.5, containing 0,05% of sodium azide as preservative. Store at + 4 °C (<10 months) or at - 20 °C (for longer period).

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,05%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Steroid Cross-reactivity of the clone XM207

Cross-reactivity, %

50% displacement of

Progesterone-11-125-Iodine)

HRP-02
Progesterone 100 100
17-Hydroxyprogesterone 1.0 10
11-Hydroxyprogesterone 25 N/A
5-alpha-pregnane-3,20 dionene 10.5 N/A
Corticosterone 0.01 N/A
Pregnenolone 0.9 N/A
Deoxycorticosterone 0.30 1
Deoxycortisol 0.03 N/A
Cortisol 0.002 N/A

For Research Use Only


MONOCLONAL ANTI-HUMAN PROGESTERONE


PRODUCT CODE : RDI-CBL 70
     $438.00/1 milligram     Larger Sizes Quoted on RequestBULK QUOTED UPON REQUEST

     $375.00/mg 5-9

     $312.00/mg 10-50mg

CLONE: 560-1

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: Ion exchange chromatography

SPECIFICITY: Progesterone - 100%

AFFINITY CONSTANT: 2 x 1010mol-1

CROSS REACTIVITY: Pregnenolone - 3%

                                       Testosterone - 1%

                                       Oestradiol - 1%

                                       Cortisol <0.1%

APPLICATIONS: Enzyme conjugation, radio-labelling or solid phase immobilization. Sensitivity of an RIA using this antibody is 5 pg/assay tube

REFERENCES: Kohen, F. & Lichter, S. Serono Symp. Pub 1. 30, 87 (1986)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 1mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20 DGE C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RE: Progesterone antisera POLYCLONAL MATERIAL

I have listed below some of the polyclonal antibodies we can supply which may work in various Elisa configurations. For a truely sensitive Elisa, you will probably need to use an amplified system (ie, use biotinylated Progesterone as a "tracer" and then HRP-Streptavidin, coupled with TMB for color generation. Please see our special Poly-HRP-Streptavidin, our HRP-Conjugate stabilizer, and our TMB-SX which when combined will allow extremely sensitive assay production. We can also supply many derivatives including a Progesterone-11-hemisucinate:Biotin (approx $1875.0/milligram) or a Progesterone-11-hemisuccinate:HRP upon request. For iodinated (RIA) assay, we also have the Progesterone-11-histamine ($1875.00/mg). Other Quotes on request.

Listed below is the available data summarized for these prospective pools of antibodies: 1: = approx titer in 125I assay.

PROGESTERONE ANTISERA(rabbit)

lot#                  ml            approx titer in 125I assay      approx backup as of  Dec 1997

#Prog-25        90ml         1:4 million                                 $1250.00/ml

#Prog-45        700ml        1:2 million                                 $1250.00/ml

#Prog-55        800ml        1:4 million                                 $1250.00/ml

#Prog-65        350ml        1:2 million                                 $1250.00/ml

#Prog-95        150ml        1:2 million                                 $1250.00/ml

#Prog-101       465ml       1:4 million                                 $1250.00/ml

Bulk pricing will be quoted on request.

All polyclonal lots were generated in rabbits against a combination P11-P3 antigen.


Sample Pricing: 0.1ml (100 microliters)=$312.00

   

Shipping/handling $44.00/order for Fed X delivery . Allow 1 week for delivery from time of order.


We also have a Progesterone-3 antibody which is used in our 3H assay system, cat#P1604 $38.00/vial lot#01719 5 microliters neat antisera lyophilized= 50 tests. This antibody is available in bulk at $1250.00/ml.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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