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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mab to Synaptopodin

cat#RDI-PRO65194   $375.00

Clone                               G1D4

Category                           Mouse monoclonal

Immunoglobulin Class       IgG1

Form/Purification              Cell culture supernatant

Antigen                               isolated rat kidney glomeruli

Clinical Application/Specificity The antibody recognizes differentiated podocytes(glomerular visceral epithelial cells) in vivo and in vitro. Weaker additional reaction with arterial endothelial cells. Does not react with parietal cells.

Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation- dependent expression in cultured hippocampal neurons.

Polypeptide Reacting: Reacts specifically with synaptopodin, a prolin-rich actin binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kd and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74kd,pI9.27. In SDS-PAGE the antigen appears as 100kd polypeptide in brain and 110kD polypeptide in kidney(the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44kd degradation fragment of synaptopodin.

Antigen Recognizied: Human, bovine, rat, mouse, guinea pig, gerbil (negative with rabbit, frog chicken)

Reactivities on cultured Cell Lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelial), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma

Application o Immunoblotting a9pprox 1:10-1:50)

                     o Immunohistochemistry on frozen tissue, paraffin embedded (paraffin staining after

                      high temp/microwave treatment )

                      * immunofluorescence

Working Dilution: Ready to Use for immunohistochemistry    1 Hr at RT

Storage At 2-8 DEC C

Volume 5 ml

References

Mundel, P. Gilbert P, and W Kriz. Podocytes in Glomerlus of Rat Kidney Express a Characteristic 44 kD Protein. J. Histochem and Cytochem Vol 39 No 8:1047-1056 (1991)

Mundel P and W Kritz. Structure and function of podocytes:an update Anat Embroyol 192:385-397 (1995)

Mundel P, Reiser J, Kris W. Phenotypic conversion and differentation of human and rat podocytes in vitro. J. Am Soc Nepherol 8:8978-705 (1997)

Mundel P, Heid HW, Mundel TM, Kruger M, Reiser J, Kriz W. Synaptopodin, an actin associated protein in telencephalic dendrites and renal podocytes. J. Cell Biol Vol 139 (1):193-204 (1997)

Mundel P, Reiser J, Zuniga Boree A, Davison G, Pavenstadt H, Kriz W, Zeller R. Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of condionally immortalized mouse podocyte cell lines, Exp Cell RES 236:248-258(1997)

Kobayashi N, et al:Non uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes. J Cell Biol 143:1961-1970 (1998)

Barisoni L et al, The dysregulated podocyte phenotype:a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy. J Am Soc Nephrol 10:51-61 (1999)

Kihara, I et al, :Origin of hyperplastic epithelial cells in idiopathic collasping glomerulopathy. Histopathology 34(6):537-547 (1999)

For In Vitro Research Use Only


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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