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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-Desmocollin 3
Mab to Desmocollin 3
cat#RDI-PRO65193, new cat#: 10R-D103a $412.50/5ml
Clone: Dsc3-U114
Category: Mouse monoclonal
Immunoglobulin Class: IgG1
Form/Purification Cell culture supernatant
Antigen: Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of human desmocollin 3. (VHGAPFYFSLPNTSPEISRLWSLTKVBC).
Clinical Application/
Specificty: Mab localizes Dsc3 present in · all living epidermal layers · in glandular duct cells
· in basal matrix cells and outer root sheath of hair follicles
· basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal
layer of bladder urothelium and complex epithelium of trachea · thymic reticulum cells
All simple epithelia are negative. Intercalated disks of myocardium are negative.
Reactivities on Cultured Cell Lines: Most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT).
Application Suitable for · immunohistochemistry on frozen and paraffin- embedded sections
· immunoblotting
(Western)
Recommendations: For better results on frozen sections, preincubation
(directly after fixation) with 0.05- 0.2% Triton X100, for 5-10 min, depending
on tissue type, is recommended. SEE REF#2.
Storage At 4°C
Volume 5 ml
Polypeptide Reacting 109 kD and 100 kD polypeptides (desmocollin 3
splice isoforms at pI 5.2)
from human epidermal desmosomes.
Antigen Recognized in Species (tested so far) :Specific for human desmocollin 3; negative with bovine and rodent material.
Working Dilution Ready-to-use for immunohistochemistry
Incubation Time 1 h at RT for immunohistochemistry
References
1. Nuber, U.A., Schäfer, S., Stehr, S., Rackwitz, H.-R., Franke, W.W. (1996). Patterns of desmocollin synthesis in human epithelia: Immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells. Eur. J. Cell Biol., 66:69-74.
2.Schafer S, Stumpp S., Franke W.W. (1996) Immunologcial identification and characterization of the desmonal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108.
3. Hatzfield M et al, J Cell Biol 149:209-222 (2000)
4. Moll I et al, Arch Dermatol Res 291, 437-446 (1999)
5. Kurzen H et al, Differentiation 63, 295-304 (1998)
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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