rev:June 14, 1999

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-Desmocollin 1  (see also desmocollin 2 & 3 antibodies)

Mab to Desmocollin 1   cat#RDI-PRO65192   $375.00/vial

Clone                             Dsc1-U100

Category                        Mouse monoclonal

Immunoglobulin              Class IgG1

Form/Purification           Cell culture supernatant

Antigen                          Synthetic peptide corresponding to sequence present within the intracellular part of human                                                 desmocollin 1.

Clinical Application/ Mab localizes Dsc1 present in suprabasal layers of interfollicular epidermis

· specific cell layers (e.g. in hair follicle root sheath)

· Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

All simple epithelia are negative.

Reactivities on Cultured                             Positive on primary keratinocytes, however,

Cell Lines (tested so far)                           restricted to local piles of differentiated cells.

Application Suitable for

o immunohistochemistry on frozen and paraffin embedded sections

o immunoblotting (Western)

Storage At 4°C

Volume 5 ml

Polypeptide Reacting 111 kD (form "a") and 103 kD (form "b") polypeptides (desmocollin 1 splice forms, both at pI 5.2) from human epidermal desmosomes.

Antigen Recognized in Species (tested so far)               Specific for human desmocollin 1;

                                                                                       negative with bovine and rodent material.

Recommendation: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-.0.2% triton X-100, for 5-10 min, depending upon tissue type (see ref #2). Without detergent pretreatment a cytoplasmic component might cross react (e.g. in fibroblasts)

Working Dilution Ready-to-use for immunohistochemistry

Incubation Time 1 h at RT for immunohistochemistry

References Nuber, U.A., Schäfer, S., Stehr, S., Rackwitz, H.-R., Franke, W.W. (1996). Patterns of desmocollin synthesis in human epithelia: Immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells. Eur. J. Cell Biol. 66:69-74

-Schafer, S et al (1996)>immunological identification and characterization of the desmosomal cadherin DsG2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108.

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


or (800) 370-2222

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