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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Plakophilin 1  (two clones see below)
Mab to Plakophilin 1

cat#RDI-PRO65160    $375.00/vial

Clone PP1-5C2

Category Mouse monoclonal

Immunoglobulin Class IgG1

Form/Purification Cell culture supernatant

Antigen Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)

Clinical Application/ Specificity Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, adenocarcinoma tested so far are negative!

Application - Western blot technique

- Immunohistochemistry on cryostat and paraffin-embedded tissue sec-tions (P)

- ELISA

Storage At 4°C

Volume 5 ml

Polypeptide Reacting         Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine)

Antigen Recognized in Species (tested so far)  Human; cow, pig, Xenopus laevis

Reactivities on Cultured Cell Lines (tested so far)  HaCaT, A-431

Working Dilution Ready-to-use for immunohistochemistry

Incubation Time 1 h at RT (forzen sections) or overnight at 2-8°C (for paraffin sections)

Recommendations: For better resolution on frozen sections, preincubate (directly after fixation) with 0.05%-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended (see ref 3)

    -For delicate tissue like liver and gastrointestinal material , preincubation with saponin is preferred to triton.

    - For immunolocalization studies on electron microscopic level, preincubation with 0.1-1% saponin is recommended.


References

1. Heid H.W., Schmidt A., Zimbelmann R., Schäfer S., Winter-Simanowski S., Stumpp S., Keith M., Figge U., Schnölzer M., Franke W.W. (1994). Cell type-specific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 ("band 6 protein"). Differentiation, 58:113-131

2. Schmidt A., Heid H.W., Schäfer S., Nuber U.A., Zimbelmann R., Franke W.W. (1994). Desmosomes and cytoskeletal architecture in epithelial d differentiation: cell type-specific plaque components and intermediate filament anchorage. Eur. J. Cell Biol., Eur J Cell Biol 65:229-245 (1991)

3 Schafer S, Stumpp S., Franke W.W. (1996) Immunological identification and characterization of the desmosomal cadherin D2g2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108

4. Moll I et al:The distribution of the desmosomal preotein, Plakophilin 1 in Human Skin and Skin Tumors. J. Invest Derm, Vol 108, 139-141 (1997)

For In Vitro Research Use Only


Mab to Plakophilin 1

cat#RDI-PRO65161     $344.00/vial

Clone   PP1-2D6

Category Mouse monoclonal

Immunoglobulin Class IgG1

Form/Purification Cell culture supernatant

Antigen Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)


Clinical Application/

Specificity Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.


Application - Western blot technique

- Immunohistochemistry on cryostat sections

- ELISA


Storage At 4°C


Volume 5 ml

Polypeptide Reacting Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine)


Antigen Recognized in Species (tested so far)  Human; cow, pig, chicken


Reactivities on Cell lines (tested so far) Human Cultured HaCaT, A-431


Working Dilution Ready-to-use for immunohisto-chemistry

Incubation Time 1 h at RT or overnight at 4°C

Recommendations: For better resolution on frozen sections, preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 mon, depending on tissue type, is recommended (see ref#3)

For delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred to Trioton

For immunolocalization studies on electron microscopic level, preincubation with 0.1-1% saponin is recommended.

References

Heid H.W., Schmidt A., Zimbelmann R., Schäfer S., Winter-Simanowski S., Stumpp S., Keith M., Figge U., Schnölzer  ., Franke W.W. (1994). Cell type-specific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 ("band 6 protein"). Differentiation, vol 58 (2).

Schmidt A., Heid H.W., Schäfer S., Nuber U.A., Zimbelmann R., Franke W.W. (1994). Desmosomes and cytoskeletal architecture in epithelial differentiation: cell type-specific plaque components and intermediate filament anchorage. Eur. J. Cell Biol., 65:229-245 (1994)

Schafer, S, Stumpp S, Franke WW. (1996) Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108

Moll I., Kurzen H., Langbein L and Franke WW:The distribution of the desmosomal Protein, Plakophilin 1, in Human Skin and Skin Tumoris. J Invest Derm, Vol 108, 139-141 (1997)

For In Vitro Research Use Only-Not For Use in Diagnostics


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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