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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Desmoglein 2


Mab to Desmoglein 2

cat#RDI-PRO65159    $312.00/vial

-also available purified cat#RDI-PRO61059 $375.00/50ug Bulk on Request

Clone                            10G11

Category                       Mouse monoclonal

Immunoglobulin Class  IgG1

Form/Purification          Cell culture supernatant/ascites

Antigen Recombinant

peptide                           (220aa of 2nd and 3rd extracellular repeat domain)

Clinical Application/ Specificity Antibody recognizes desmoglein 2 present in all desmosome possessing tissues; stratified and single-layered polar epithelia and tumors derived therefrom as well as heart muscle and dendritic reticulum cells of lymphatic follicles.

    With stratified squamous epithelia only desmosomes connecting the basal, proliferative cell layer contain detectable desmoglein 2, it seems to be absent from all suprabasal cell layers (these layers are, however positive for desmoglein 1 and 3). In basal epithelial cells,desmoglein 2 expression is generally very low.

Application              o  Western blot technique

                                 o Immunohistochemistry on cryostat* and paraffin** sections

                                  o ELISA

* for better resolution on frozen sections preincubation (direclty after fixation) with 0.05-0.2% Triton X-100, for 5-10 minutes, depending on tissue type, is recommended (see ref#3)

** only when using the microwave method (high temp release)

Storage                     At 4°C

Volume                     5 ml

Polypeptide Reacting Desmoglein 2 (1069aa; calculated mol. wt. 116,760)

Antigen Recognized in Species Human (not with rat or mouse)

Working Dilution Ready-to-use for immunohistochemistry

Incubation Time 1 h at RT or overnight at 4°C

Reactivities on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7

References

Schäfer S, Koch P.J. , Franke W.W. (1994) Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. Exp. Cell Res. 211:391-399.

Franke W.W., Nuber U., Schmidt A., Schäfer S. (1994). Desmosomes - dual junctional principles of intra- and supracellular order in epithelial differentiation and tissue formation. Verh. Dtsch. Ges. Path., 8:8-14

Schäfer S, Stumpp S., Franke W.W. (1996) Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation, 60:99-108

Demlehner M.P., Schäfer S., Grund C., and Franke W.W. (1995) Continual Assembly of Half-Desmosomal Structures in the Absence of Cell Contacts and Their Frustrated Endocytosis: A Coordinated Sisyphus Cycle. Journ. Cell Biol., Vol. 131,. No. 3:745-760.

Schäfer, S., Stumpp, S., Franke, W.W.: Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation 60: 99-108 (1996).


For In Vitro Research Use Only-Not For Use IN Diagnostics


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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