rev:  February 9, 2000

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Multi-Epitope Cocktail to Lamins

(intermediate filament proteins of the nucleus)

cat#RDI-PRO65147     $250.00/vial      $188.00/vial 3+

Clone(s)                X67, X167, X233 (individual abs on request-see below)

Category               Mouse monoclonals

Reagent                Ascites (diluted) or hybridoma culture supernatant

Ig Class                all IgG1

Immunogen           Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells )

Specificity/Clinical Application The mab cocktail decorates the karyoskeleton, ie, the intermediate filament equivalent of the nucleus

Polypeptide(s) Reacting/Specificity: -Lamin isotypes of Mr60-75kD

Application · immunofluorescence microscopy

                      - immunoblotting (western)

                      Antigen Recognized                  X67               X167                 X223

                      Xenopus laevis                        LA1,L1,L11     LA,L11             L11,L111

                      Bovine                                     LA,C               LA,B1,B2,C      LB1,B2

                      Mouse                                      n.d                   n.d.                    LB2

                      Rat                                             -                      -                         +

                      Human                                     La,C                 La,C                  LB1,B2

                      Trout                                       LA,L1,L11        LA,L11             LII, LIII

                      Rat kangaroo                               -                      -                      L11, L111

Working Dilution Ready to use for immunohistochemistry.

Incubation Time         1 h at room temperature

Storage                       At 2-8 DEG C

Volume                       5 ml (contain 0.09% NaN3)

Ref:

Krohne,G. and Benavente,R:The Nuclear Lamins. A Multigene Family of Proteins in Evolution and Differentiation. Exp. Cell Res 162, 1-10 (1986)

-Franke, W.W. Nuclear Lamins and Cytoplasmic Intermediate Filament Proteins:A growing Multigene Family. Cell 48,3-4(1987)

Hoger, T.H., et al:Immunolocalization of Lamins in the thick nuclear amina of human synovial cells. Europ J Cell Biol 54,150-158 (1991)

Hoger et al;Characterization of a second highly conserved B-type lamin present in cells previously through to contain only a single B-type lamin. Chromosoma 99, 379-390 (1990)


For In Vitro Research Use Only-Not For Use In Or On Humans or Animals-Not For Diagnostics


To order individual clones:

Clone X67     cat#RDI-PRO65147-67      $250.00/5ml

Clone X167    cat#RDI-PRO65147-167        "

Clone X223    cat#RDI-PRO65147-X223      "

$188.00/vial 3 or more


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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