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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Symplekin

Mab to Symplekin

cat#RDI-PRO651100     $375.00/vial  

Clone Determination:     Sym-TJ-E150

Category:                       Mouse monoclonal

Immunoglobulin Class:   IgG1

Purification:                   Hybridoma culture supernatant

Immunogen:                    Human symplekin

Specificity:                   The mab reacts specifically with symplekin ( a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelial) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis, non-epithelia such as fibroblasts, endothelia, lymphocytes).

Polypeptide Reacting: Symplekin, 126.5 kD polypeptide (calculated from a sequence data); apparent M 150 kD (after SDS-PAGE); pl 6.5

Antigen recognized in species (tested so far) Human rat  ; negative with bovine, mouse, Xenopus laevis

Reactivities on Cultured Cell Lines (tested so far): Carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG/343 MG (glioma). K 562(erythroleukemia), RVF (rat vascular smooth muscle cells)

Application: Immunoblotting. Immunohistochemistry: For nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash

Working Dilution: Ready-to-use for immunohistochemistry

Storage: At 2 - 8'C

Volume: 5ml

References:

1. Keon BH, Schafer S. Kuhn C. Grund C, and Franke WW.Symplekin, a Novel Type of Tight Junction Plaque Protein, J Cell Biol Vol 134, 4:1003-1018

2. Zahraoui A et al, J Cell Biol 151, F31-F36 (2000)

3. Eur J Cell Biol 81, 419-435 (2002)

4. Eur J Cell Biol 82, 385-400 (2003)

For In Vitro Research Use Only. Not supplied for use in human diagnostic or therapeutic procedure


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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