rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below purified and biotin conjugate
see laso polyclonals from the main list
Mab to Human LDL Receptor
(low density lipoprotein receptor)
cat#RDI-PRO61087, new cat#: 10R-L104a $412.50/vial
-also available Biotin labeled cat#RDI-PRO61587, new cat#: 61R-L104aBT $517.00/1ml
Clone
IgG-C7
Category
Mouse monoclonal
Form/Purification
affinity purified
Ig Class
IgG2bk
Immunogen
Purified bovine adrenal cortex LDL Receptor
Clinical Application/
Specificity
Suitable for analysis of LDL
receptor function in patients with familial
hypercholesterolemia. The antibody recognizes an epitope in the region of
re-peat #1 of the ligand binding region. Addition of 15 nM
antibody results in inhibition of half-maximal LDL-binding (cf. Beisiegel et
al. 1981).
Application
· Immunoblotting
approx 1 to 0.1ug/ml with enhanced chemiluminescence detection
(due to low affinity of this antibody, extended incubation-overnight 4 DEG C or longer is recommended)
Reactivity(species)
Human, Bovine (not rat, mouse, hamster, dog and
rabbit)
Dilution dilute 1:5 with PBS, pH 7.4 for iimmunohistochemistry
Dilution Buffer:
PBS with 0.5% BSA and 0.1% Na-Azide
Storage/Stability After reconstitution with 1ml distilled water, the antibody is stable for
a min 1 year when stored at 4 DEG C
Quantity: 50ug (lyophilized) contains BSA and NaN3.
References:
U Beisiegel et al, Biol Chem Vol 256, 22,
11923-11931 (1981)
For In Vitro Research Use Only-Not For Use In Or On Humans or Animals-Not For
Diagnostics
Mab to Human LDL RECEPTOR:Biotin Conjugated (low density lipoprotein receptor)
cat#RDI-PRO61587 $469.00/vial
Clone IgG-C7
Category Mouse monoclonal
Form/Purification affinity purified, biotin conjugated
Ig Class IgG2bk
Immunogen Purified bovine adrenal cortex LDL receptor
Clinical Application/
Specificity Suitable for analysis of LDL receptor function in patients with familial hypercholesterolemia. The antibody recognizes an epitope in the region of repeat #1 of the ligand binding region. Addition of 15 nM antibody results in inhibition of half-maximal LDL-binding (cf. Beisiegel et al. 1981).
Antigen Recognized Human, bovine; negative with rat, mouse, hamster
in Species (tested so far) (chinese hamster ovary cells), dog, rabbit
Suitable for FACS
Immunohistochemistry
Immunoblotting
Dilution Buffer PBS with 0.5% BSA
Antigen Recognized in
Species Tested so far: Human, bovine; negative with rat, mice, hamster, dog and rabbit
Dilution dilute 1:10 with PBS, pH 7.4
Dilution Buffer: PBS with 0.5% BSA
Storage/Stability at 2-8 DEG C
Quantity: 1 mL (conc. 0.5 mg/mL)
Reference
1. Beisiegel U, Schneider WJ, Goldstein JL, Anderson RGW, Brown MS: Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J Biol Chem 256, 11923-11931 (1981)
2. Beisiegel U, Schneider WJ, Brown MS, Goldstein JL. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J Biol Chem 257, 13150-13156 (1982)
3. Schmitz G, Brüning T, Kovacs E, Barlage S. Fluorescence flow cytometry of human leukocytes in the detection of LDL receptor defects in the differential diagnosis of hypercholesterolemia. Arteriosclerosis & Thrombosis 13, 1053-1065 (1993)
4. Virgolini I, Li S-R, yang Q, Koller E, Sperr WR, Leimer M, Angelberger P, Nimpf J, Schneider W, Valent P. Characterization of LDL and VLDL binding sites on human basophils and mast cells. Arteriosclerosis, Thrombosis & Vascular Biology 15, 17-26 (1995)
For research use only, not for therapeutic or diagnostic use! Rev: February 1, 2005
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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