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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DESMOPLAKIN 1   (see below monoclonal and polyclonal)
Mab to Desmoplakin 1

cat#RDI-PRO61024   $375.00/vial

Clone Determination DP 2.17

Category Mouse monoclonal

Immunoglobulin Class IgG1

Purification SpAss-Protein A (recombinant)

Antigen: Bovine desmoplakin 1

Specificity: DP2.17 shows distinct punctate membrane staining of epithelia. DP2.17 reacts with the rod fragment of desmoplakin 1

Application Suitable for frozen tissue and Immunoblotting (Western)

NEW: Suitable for paraffin-embedded tissue when using the MICROWAVE method.

Stability/Storage One year after reconstitution when stored at 4°C

Quantity: 50ug lyophilized

Polypeptide Reacting Desmoplakin no. 1 (Mr 250 000 polypeptide of desmosomal plaques)

Antigen Recognized in species (tested so far)   Human, bovine, rat, chicken, mouse

Reactivities on Cultured Several human Ca. cell lines: MCF-7, Cell Lines (tested so far) A-431, TR-146; bovine cells: MDBK, BMGE

Tumors Specifically Detected Primary and metastatic Ca.; meningioma

Working Dilution When reconstituted with 1ml distilled water dilute 1:10 with PBS (pH 7.4) for immunohistochemical application

Incubation Time 1hr at RT

References

Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Biol. 101, 1442-1454 (1985).

Franke, W.W., Cowin, P., Grund, C., Kuhn, C. and Kapprell, H.P.: The endothelial junction. The plaque and its components. In: Endothelial Cell Biology. N. Simionescu and M. Simionescu, eds., Plenum Publishing Corp., New York, pp. 147-165 (1988).

Moll, I.and Moll, R., Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res, 283, 300-309 (1991).

Moll, R., Cowin, P., Kapprell, H.P. and Franke, W.W.: Desmosomal proteins: New markers for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986)

Schmelz, M. and Franke W.W.: Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: The syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-289 (1993)

For Research Use only


Pab to Desmoplakin 1

Cat#RDI-PRODP1 $470.00

Source      Guinea pig

Form        Whole antiserum

Antigen Bovine desmoplakin 1 (prepared from SDS-polyacryl-amide gels)

Specificity DP-1 antiserum shows distinct punctate membranestaining of epithelia. In Western blot analysis theantiserum reacts specifically with the desmoplakin I polypeptide.

Application Suitable for frozen tissue and for paraffin-embedded tissue (improved when using the MICROWAVE method) Immunoblotting (Western)

Working Dilution 1:100 for immunohistochemistry

                             1:50 for paraffin-embedded tissue

                             1:1000 for Western blotting

Incubation Time 1 h at RT; extended with paraffin sections

Storage At 2 - 8°C

Quantity 50 µL

Reference

Troyanovsky S.M., Troyanovsky R.B., Eshkind L.G., Krutovskikh V.A., Leube R.E. and Franke W.W.: Identification of plakoglobin-binding domain in desmoglein and its role in plaque assembly and intermediate filament anchorage. J.Cell Biol. 127, 151 - 160 (1994)

For In Vitro Research Use Only


Mab to Desmoplakin 1&2

cat#RDI-PRO61003    $375.00/vial   (new cat# for old cat#RDI-CBL173)


Clone Determination     DP1&2-2.15


Category     Mouse monoclonal

Immunoglobulin Class IgG1

Purification Ion-exchange chromatography

Antigen Bovine desmoplakin 1&2

Specificity DP1&2-2.15 shows distinct punctate membrane staining of different epithelia.

Application Suitable for frozen tissue and Immunoblotting (Western)

Stability/Storage One year after reconstitution when stored at 4°C

Quantity 50 µg (lyoph.)

Polypeptide Reacting Desmoplakin 1 and 2 (Mr 250 000 and 215.000)

Antigen Recognized in Species tested so far:  Human, bovine, rat, mouse, chicken

Reactivities on Cultured cell lines (tested so far) : Several human Ca.Cell Lines : MCF-7, A-431; rat MH1C1 cell line; bovine cells: MDBK, BMGE,

Tumors Specifically Detected Primary and metastatic Ca.; meningioma


Application Suitable for frozen tissue Immunoblotting (Western) Cytological material


Working Dilution When reconstituted with 1ml distilled water dilute 1:10 with PBS (pH 7.4)


Incubation Time 1h at RT


References

Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).

Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).

Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R., Artileb, U. and Denk, H.: Immunocytochemical identification of epithelium-derived human tumours with antibodies to desmosomal plaque proteins. Proc. Natl. Acad. Sci., USA, 80, 543-547 (1983).

Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990).

Moll, I. and Moll, R., Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res, 283, 300-309 9(1991).

Moll, R., Cowin, P., Kapprell, H.P. and Franke, W.W.: Desmosomal proteins: New markers for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986).

Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz, L.A. and Franke, W.W.: The hemidesmosomal plaque. I. characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation 45, 207-220 (1990).

Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).


Schmelz, M. and Franke W.W.: Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: The syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-289 (1993)

For In Vitro Research Use Only-Not for use in or on humans or animals Not supplied for use in human diagnostic or therapeutic procedures


Multi-Epitope Cocktail to Desmoplakin 1&2

cat#RDI-PRO65146       $281.00/vial

Clone Determination DP1&2-2.15 , DP1-2.17,  DP1&2-2.20


Category Mouse monoclonals


Immunoglobulin Subclass All IgG1


Purification Ascites or hybridoma culture supernatant


Antigen Bovine desmoplakins 1&2


Specificity The antibody cocktail shows distinct punctate membrane staining of different epithelia


Application Suitable for frozen tissue Immunoblotting (Western) and paraffin sections (using antigen release methods/microwave etc)

Storage At 4°C


Volume 5 ml


Polypeptide(s) Reacting Desmoplakins 1 and 2 (Mr 250.000 and 215.000)

Antigen Recognized in Species (tested so far) Human, bovine, rat, mouse, and chicken


Reactivities on Cultured Cell Lines (tested so far) Several human CA lines: MCF-7, 431;

                                     rat MH1C1 cell line; bovine cells: BMGE, MDBK

Tumors Specifically Detected Primary and metastatic CA; meningioma

Application Suitable for frozen tissue Immunoblotting (Western) Cytological material (paraffin sections using microwave release)

Working Dilution Ready-to-use for immunohistochemistry

Incubation Time 1 h at RT

Storage At 4°C

References

Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).


Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).


Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R., Artileb, U. and Denk, H.: Immunocytochemical identification of epithelium-derived human tumours with antibodies to desmosomal plaque proteins. Proc. Natl. Acad. Sci., USA, 80, 543-547 (1983).


Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990).


Moll, I. and Moll, R., Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res, 283, 300-309 9(1991). for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986).


Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz, L.A. and Franke, W.W.: The hemidesmosomal
plaque. I. characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation 45, 207-220 (1990).


Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).

For In Vitro Research Use Only


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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