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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Plakoglobin =gamma catenin (2 clones) (see also related catenin antibodies)


Mab to Plakoglobin (identical to gamma-catenin)

cat#RDI-PRO61005    $375.00/vial      $312.00/vial 5+

Clone Determination      PG 5.1

Category                        Mouse monoclonal

Immunoglobulin Class   IgG2b

Purification Protein A   affintiy chromatography

Antigen                         Plakoblobin, "band 5" protein from bovine snout epidermis

Specificity                    PG 5.1 represents an excellent marker for all intercellular adhering junctions

Application Suitable for · frozen tissue

                                        · immunoblotting (Western)

                                       · cytological Material

                                      -immunofluorescence microscopy

Stability/Storage          One year after reconstitution when stored at 2-8°C

Quantity                        50 µg (lyoph.) w/0.5% Bsa & 0.09% NaN3

Polypeptide Reacting     MR 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to gamma-catenin(). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin.

Antigen Recognized in      Human, bovine, rat, chicken, mouse

Species (tested so far)

Reactivities on Cultured Cell cultures forming monolayers Cell Lines (tested so far)

Structures and Tissues          All forms of intercellular adhering

Specifically Detected junctions, such as: desmosomes of epithelial & myocardial cells (incl. culture cells); zonulae & fasciae adherents of epithelia, endothelia of blood vessels &  myocardial cells; adherent-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis)

Working Dilution         When reconstituted with 1ml distilled water dilute 1:5 (for immunofluorescence) approx 1:10 for histochemistry, Titer for each application.

Incubation Time 1 h at RT

References

Cowin, P., Kapprell, H.P. Franke, W.W.:, Tamkun, J. and Hynes, R.: Plakoglobin: A protein common to different kinds of intercellular adhering junctions. Cell 46, 1063-1073 (1986)

Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990) Wahl, J.K., Sacco, P.A., McGranahan-Sadler, M., Sauppé, L.M., Wheelock, M.J., Johnson, K.R.: Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J. Cell Science 109, 1143-1154 (1996)

Chitaev NA, Leube RE, Troyanovsky RB, Eshkind LG, Franke WW, Troyanovsky SM. The binding of plakoglobin to desmosomal cadherins: Patterns of binding sites and topogenic potential. J Cell Biol 133(2):359-369 (1996)

Luft FC. Wingless armadillos and their clinical relevance. J. Mol. Med. 75:1-2 (1997)

Cerdà J, Reidenbach S, Prätzel S, Franke WW: Cadherin-catenin complexes during zebrafish oogenesis: Heterotypic junctions between oocytes and follicle cells. Biol.Reproduction 61, 692-704 (1999)

Caldelari R, de Bruin A, Baumann D, Suter MM, Bierkamp C, Balmer V and Müller E: A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris. J. Cell Biol. 153, 823-834 (2001)

For In Vitro Research Use Only


Mouse anti-Plakoglobin Monoclonal ab

cat# RDI-PLAKOGabm         $438.00/vial 50ug

       RDI-PLAKOGamb-1      $750.00/vial 150ug

Background: Plakoglobin (also known as gamma catenin) was originally identified as a component of desmones where it is found in association with desmoglein. Gamma-catenin and Beta-catenin are closely related proteins and have significant homology with theDrosphilia armadillo protein. In addition to complexing with E-cadherin, G-catenin and B-catenin have been observed in association with the intracellular domain of N-cadherin. It has been proposed that one molecule of alpha-catenin and at least one molecule of B-catenin and G-catenin simultaneously bind to a single cadherin molecule. Recently, a 19-amino acid sequence of desmoglein (Dsg1) has been recognized as critical for binding of G-catenin. This region has significant homology to catenin-binding domain of classical cadherins, suggesting a common  mechanism for G-catenin localization at both adherens junctions and desmosomes.

isotype: mIgG2a

package: 50ug or 150ug

antigen: a 19.9 kDa fragment corresponding to amino acids 553- 738 of the human gamma-catenin protein

USe: Suitable for western blot on human, dog, rat, mouse and chick, using approx 1 to .04ug/ml ab on total cell lysate. Major band at Mw 82 kDa.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Hinck, L. et al. (1994) J. Cell Biol. 125: 1327

                  Mathur, M. et al. (1994) J. Cell Biol. Chem. 269: 14075

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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