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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESMOPLAKIN 1 & 2 (two formulations/clones see below)
Mab to Desmoplakin 1&2
cat#RDI-PRO61003, new cat#: 10R-D108ax $412.50/vial
Clone Determination DP1&2-2.15
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification affinity chromatography
Antigen Bovine desmoplakin 1&2
Specificity DP1&2-2.15 shows distinct punctate membrane staining of
different epithelia
Application Suitable for -immunohistochemistry and -immunofluorescence on
frozen tissue
-Immunoblotting (Western)
Stability/Storage One year after reconstitution when stored at 4°C. For
longer aloiquot an dstore at -20 DEG C. Avoid frequent freeze thaw cycles
Quantity 50 µg (lyoph.) contains 0.5% BSA and 0.09% NaN3.
Polypeptide Reacting Desmoplakin 1 and 2 (Mr 250 000 and 215.000)
Antigen Recognized in Human, bovine, rat, mouse, chicken
Species (tested so far)
Reactivities on Cultured cell lines (tested so far) Several human Ca. cell lines: MCF-7, A-431, rat MH1C1 cell line; bovine cells: MDBK, BMGE
Tumors Specifically Detected Primary and metastatic Ca.; meningioma
Application Suitable for frozen tissue Immunoblotting (Western) Cytological
material
Working Dilution When reconstituted with 1ml distilled water dilute 1:10 with
PBS (pH 7.4)
Incubation Time 1h at RT
References
Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).
Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).
Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R.,
Artileb, U. and Denk, H.: Immunocytochemical identification of
epithelium-derived human tumours with antibodies to desmosomal plaque proteins.
Proc. Natl. Acad. Sci.,
Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990).
Moll,
Moll, R., Cowin, P., Kapprell, H.P. and Franke, W.W.: Desmosomal proteins: New markers for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986).
Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz,
Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).
Schmelz, M. and Franke W.W.: Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: The syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-289 (1993)
For In Vitro Research Use Only-Not for use in or on humans or animals
For In Vitro Research Use Only-Not for use in or on humans or animals Not supplied for use in human diagnostic or therapeutic procedures
Multi-Epitope Cocktail to Desmoplakin 1&2
cat#RDI-PRO65146, new cat#: 10R-D108a $313.50/5ml
Clone Determination DP1&2-2.15 , DP1-2.17, DP1&2-2.20
Category Mouse monoclonals
Immunoglobulin Subclass All IgG1
Purification Ascites or hybridoma culture supernatant
Antigen Bovine desmoplakins 1&2
Specificity The antibody cocktail shows distinct punctate membrane staining of
different epithelia
Application Suitable for frozen tissue Immunoblotting (Western) and paraffin
sections (using antigen release methods/microwave etc)
Storage At 4°C
Volume 5 ml
Polypeptide(s) Reacting Desmoplakins 1 and 2 (Mr 250.000 and 215.000)
Antigen Recognized in Species (tested so far) Human, bovine, rat, mouse, and chicken
Reactivities on Cultured Cell Lines (tested so far) Several human CA lines:
MCF-7, 431;
rat MH1C1 cell line; bovine cells: BMGE, MDBK
Tumors Specifically Detected Primary and metastatic CA; meningioma
Application Suitable for frozen tissue Immunoblotting (Western) Cytological material (paraffin sections using microwave release)
Working Dilution Ready-to-use for immunohistochemistry
Incubation Time 1 h at RT
Storage At 4°C
References
Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).
Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B.,
Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein
in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71
(1987).
Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R.,
Artileb, U. and Denk, H.: Immunocytochemical identification of
epithelium-derived human tumours with antibodies to desmosomal plaque proteins.
Proc. Natl. Acad. Sci.,
Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and
Franke, W.W.: Identification of desmoglein, a constitutive desmosomal
glycoprotein, as a member of the cadherin family of cell adhesion molecules.
Eur. J. Cell Biol. 53, 1-12 (1990).
Moll,
Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz,
plaque.
Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of
hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992,
622-630 (1991).
-Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).
-Hatzfield M et al, The function of plakophlin 1 in assembly and actin filament organization J Cell Biol. 149, 209-222 (2000)
-Eur J Cell Biol 81, 419-435 (2002)
-EXP Cell Res 285, 114-130 (2003)
For In Vitro Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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