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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DESMOPLAKIN 1 & 2   (two formulations/clones see below)


Mab to Desmoplakin 1&2

cat#RDI-PRO61003     $375.00/vial

Clone Determination DP1&2-2.15

Category Mouse monoclonal

Immunoglobulin Class IgG1

Purification affinity chromatography

Antigen Bovine desmoplakin 1&2

Specificity DP1&2-2.15 shows distinct punctate membrane staining of different epithelia

Application Suitable for -immunohistochemistry and -immunofluorescence on frozen tissue

-Immunoblotting (Western)

Stability/Storage One year after reconstitution when stored at 4°C. For longer aloiquot an dstore at -20 DEG C. Avoid frequent freeze thaw cycles

Quantity 50 µg (lyoph.) contains 0.5% BSA and 0.09% NaN3.


Polypeptide Reacting Desmoplakin 1 and 2 (Mr 250 000 and 215.000)

Antigen Recognized in Human, bovine, rat, mouse, chicken

Species (tested so far)

Reactivities on Cultured cell lines (tested so far)  Several human Ca. cell lines: MCF-7,  A-431, rat MH1C1 cell line;                                bovine cells: MDBK, BMGE

Tumors Specifically Detected Primary and metastatic Ca.; meningioma

Application Suitable for frozen tissue Immunoblotting (Western) Cytological material

Working Dilution When reconstituted with 1ml distilled water dilute 1:10 with PBS (pH 7.4)

Incubation Time 1h at RT

References

Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).

Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).

Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R., Artileb, U. and Denk, H.: Immunocytochemical identification of epithelium-derived human tumours with antibodies to desmosomal plaque proteins. Proc. Natl. Acad. Sci., USA, 80, 543-547 (1983).

Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990).

Moll, I. and Moll, R., Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res, 283, 300-309 9(1991).

Moll, R., Cowin, P., Kapprell, H.P. and Franke, W.W.: Desmosomal proteins: New markers for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986).

Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz, L.A. and Franke, W.W.: The hemidesmosomal plaque. I. characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation 45, 207-220 (1990).

Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).

Schmelz, M. and Franke W.W.: Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: The syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-289 (1993)

For In Vitro Research Use Only-Not for use in or on humans or animals

For In Vitro Research Use Only-Not for use in or on humans or animals Not supplied for use in human diagnostic or therapeutic procedures


Multi-Epitope Cocktail to Desmoplakin 1&2

cat#RDI-PRO65146       $281.00/vial

Clone Determination DP1&2-2.15 , DP1-2.17,  DP1&2-2.20


Category Mouse monoclonals


Immunoglobulin Subclass All IgG1


Purification Ascites or hybridoma culture supernatant


Antigen Bovine desmoplakins 1&2


Specificity The antibody cocktail shows distinct punctate membrane staining of different epithelia


Application Suitable for frozen tissue Immunoblotting (Western) and paraffin sections (using antigen release methods/microwave etc)

Storage At 4°C


Volume 5 ml


Polypeptide(s) Reacting Desmoplakins 1 and 2 (Mr 250.000 and 215.000)

Antigen Recognized in Species (tested so far) Human, bovine, rat, mouse, and chicken


Reactivities on Cultured Cell Lines (tested so far) Several human CA lines: MCF-7, 431;

                                     rat MH1C1 cell line; bovine cells: BMGE, MDBK

Tumors Specifically Detected Primary and metastatic CA; meningioma

Application Suitable for frozen tissue Immunoblotting (Western) Cytological material (paraffin sections using microwave release)

Working Dilution Ready-to-use for immunohistochemistry

Incubation Time 1 h at RT

Storage At 4°C

References

Cowin, P., Kapprell, H.P. and Franke, W.W.: The complement of desmosomal plaque proteins in different cell types. J. Cell Ciol. 101, 1442-1454 (1985).


Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).


Franke, W.W., Moll, R., Müller, H., Schmid, E., Kuhn, C., Krepler, R., Artileb, U. and Denk, H.: Immunocytochemical identification of epithelium-derived human tumours with antibodies to desmosomal plaque proteins. Proc. Natl. Acad. Sci., USA, 80, 543-547 (1983).


Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. and Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990).


Moll, I. and Moll, R., Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res, 283, 300-309 9(1991). for identification and classification of tumors. Lab. Invest. 54, 4-25 (1986).


Owaribe, K., Kartenbeck, J., Stumpp, S., Magin, T.M., Krieg, T., Diaz, L.A. and Franke, W.W.: The hemidesmosomal
plaque. I. characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation 45, 207-220 (1990).


Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).

-Owaribe, K., Nishizawa, Y. and Franke, W.W.: Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp. Cell Rev. 1992, 622-630 (1991).

-Hatzfield M et al, The function of plakophlin 1 in assembly and actin filament organization J Cell Biol. 149, 209-222 (2000)

-Eur J Cell Biol 81, 419-435 (2002)

-EXP Cell Res 285, 114-130 (2003)

For In Vitro Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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