rev:     October 11, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DESMOGLEIN  (new cat# replaces old cat#RDI-CBL174)

-see also other desmoglein , desmoplakin  and more  antibodies from the alphabetical list


Mab to Desmoglein 1 + 2

cat# RDI-PRO61002 $375.00/vial Bulk quotes on request

cat#RDI-PRO61002X $2500.00/1mg (no BSA)

Clone Determination               DG 3.10

Category                                 Mouse monoclonal

Immunoglobulin                      Class IgG1

Purification                             SpAss-Protein A (recombinant)

Antigen                                   "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis

Specificity                               DG 3.10 recognizes desmoglein 1 +2 in desmosome- bearing cells; does not react with cells containing plaque-bearing junctions of non desmosomal type

Polypeptide reacting :           Mr165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Application                           Suitable for frozen tissue Immunoblotting (Western)

                                              NEW: Suitable for paraffin-embedded tissue when using the MICROWAVE method.

Stability                                One year after reconstitution when stored at 2-8C

                                              For longer storage aliquots should be kept at -20 Deg C.

Quantity                                50 µg (lyoph.) After reconstitution with 1 ml distilled water, solution will contain 0.5% BSA and 0.09% NaN3.

Antigen Recognized in Species (tested so far) Human, bovine, rat, mouse

Reactivities on Cultured Cell Lines (tested so far) A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines; bovine BMGE+H, BMGE-H, MDBK

Tumors Specifically Detected             All epithelium derived tumors, notably carcinomas, and meningiomas

Working Dilution            When reconstituted with 1ml distilled water, dilute further 1:10 with PBS, pH 7.4 for immunohistochemical application.

Incubation Time             1h at RT

References

Koch, P.J., Walsh, M.J., Schmelz, M., Goldschmidt, M.D., Zimbelmann, R. und Franke, W.W.: Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1-12 (1990)

Schmelz, M., Duden, R., Cowin, P. and Franke, W.W.: A constitutive transmembrane glycoprotein of MR 165 000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur. J. Cell Biol. 42, 184-199 (1986)

Wheeler, G.N., Parker, A.E., Thomas, C.L., Ataliotis, P., Poynter, D., Arnemann, J., Rutman, A.J., Pidsley, S.C., Watt, F.M., Rees, D.A., Buxton, R.S. and Magee, A.I.: Desmosomal glycoprotein DGI, a component of intercellular desmosomes junctions, is related to the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 88, 4796-4800 (1991)

Schmelz, M. and Franke W.W.: Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: The syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-298 (1993)

Schäfer, S., Koch, P.J. and Franke, W.W.: Identification of the Ubiquitous Human Desmoglein, Dsg2, and the Expression Catalogue of the Desmoglein Subfamily of Desmosomal Cadherins. Exp. Cell Res. 211, 391-399 (1994)

Schäfer, S., Stumpp, S., Franke, W.W.: Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation 60: 99-108 (1996).

Hatzfield M et al, J Cell Biol 149, 209-222 (2000).

Caldelari R et al, J Cell Biol 153,823-834 (2001)

For In Vitro Research Use Only. Not supplied for use in human diagnostic or therapeutic procedure


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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