rev: January 6, 2005
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-SUBPabGP $375.00/50ul
Presentation: liquid whole serum with 0.05% sodium azide
Immunogen: residues 1-11 of rat Substance P (CRPKQQFGLM)
Reactivity: human, mouse, and rat (others not tested)
Use: Histochemistry: approx 1:1000 Optimal dilutions should be determined by user for each application
Storage: Store frozen. Aliquot as undiluted antisera and immediately place at -20 DEG C. At first thaw, tighten cap, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. Stable at least 6 months at -20 DEG C. Avoid repeated freeze/thaw cycles.
Ref: Neuroscience (1978) Vol 3, 945-976
For Research Use Only
Sample Protocol: Antiserum was used on perfusion fixed tissue. Perfusion 1)calcium-free Tyrode's solution 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temp. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 DEG C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temp. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
For In Vitro Research Use Only
blocking/control peptide 20ug cat#RDI-SUBPabGP-CX $156.00
Peptides are supplied dried in a phosphate buffer pH7.4. The stock solution of the antigen can be made up using 0.1ml of deoinized water. For maximum stability, the peptide should be stored at -20 DEG C. Most peptides will be stable in solution for several days at 4 DEG C. Avoid repeated freeze/thaw cycles. Used for absorption control of antiserum binding. Antisera should be preincubated with peptide prior to incubation with tissue/sample. A 10 E-05 M peptide concentration is suggested to block antigen/antibody binding. However, optimal concnetration should be determined by the investigator using a range of peptide dilutions to eliminate or reduce immunoreactivity.
Category Guinea pig polyclonal
Form/purification: Guinea pig serum
Immunogen: Substance P conjugated to BSA
Clinical Application/Specificity: Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. Substance P- containing nerve fibers in the gut wall are particularly numerous in the mesenteric plexus and the smooth muscle layers. Substance P stimulates smooth muscle contraction, gives rise to vasodilation and in involved in sensory functions. Substance P- containing tumors arising in the ileum are often associated with the "carcinoid syndrome", characterized by flushing of the skin, diarrhea broncho-constriction and sudden drops in blood pressure. Substance P is commonly found in midgut carcinoids and some of the symptoms may be related to this peptide. Absorption with 10-100 ug Substance P and Neurokinin A per ml diluted antiserum abolishes the staining while GRP and Neurokinin B do not.
Positive Control: rat colon
Species Reactivity tested so far: Rat
Suitable for frozen sections
Reconstitution: Dissolve the antiserum in 50-100ul distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.01% NaN3.
Working Dilution: 1:200-1:400 using immunofluorescence microscopy, with overnight incubation at 2-8 DEG C.
Storage: At 2-8'C (undiluted). Reconstituted in small aliquots at -20 DEG C.
Volume: 50ul (lyoph.);
For In Vitro Research Use Only
Reference: Hardebo JE at al, Neurosceince Letters 101:39-45 (1989)
Witt M et al, Histochem Cell Bio 109 (3) 281-291 (1998)
Category Rabbit polyclonal
Form/Purification Undiluted rabbit serum
Immunogen Synthetic Substance P 4-11, conjugated to thyroglobulin using CDI
Description/Specificity Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. It stimulates smooth muscle contraction, gives rise to vasodilation and is involved in sensory functions. Substance P-containing tumors arising in the ileum are often associated with the carcinoid syndrome, characterized by flushing of the skin, diarrhea, broncho-constriction and sudden drops in blood pressure. Substance P is commonly found in the midgut carcinoids and some of the symptoms may be related to this peptide.
Absorption with 10 - 100 µg SP 1-4 per ml diluted antiserum abolishes the staining.
Positive Control Frozen sections of rat colon
Reaction with Species (tested) Human, rat
Application · Immunohistochemistry
· Suitable for frozen and paraffin-embedded* tissue
*no proteolytic treatment required
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:400 - 1:800 using PAP, with overnight incubation at 2-8°C.
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
Hardebo JE et al. Origins of substance P- and calcitonin gene-related peptide-containing nerves in the internal carotid artery of rat. Neuroscience letters 101:39-45 (1989)
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266