rev:    December 8, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Histidine Decarboxylase  (see 2 polyclonals below)
Polyclonal Antibody to Histidine Decarboxylase

Cat#RDI-PRO16045    $625.00

Category                   Rabbit polyclonal

Form/Purification    Undiluted rabbit serum (lyoph.)

Immunogen               Recombinant histidine decarboxylase produced in E.Coli

Description/Specificity     Histidine decarboxylase is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in the mast cells. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.

Positive Control Stefanini-fixed frozen sections of rat fundus

Species tested so far         Rat, mouse, dog and guinea pig

Application · Immunofluorescence microscopy

                   · Suitable for paraffin and frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water,and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:2000 - 1:3000 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (freeze-dried) or reconstituted in small aliquots at -20°C

Quantity / Volume 50 µl (lyoph.)

References

Dartsch c. et al.: Recombinant expression of rat histidine decarboxylase: Generation of antibodies useful for Western blot analysis. Int. J. Biochem. & Cell Biol. 30, 773-782 (1998)

Lindström E. et al.: Neurohormonal regulation of histamine and pancreastatin secretion from isolated rat stomach ECL cells. Regulatory Peptides 71, 73-86 (1997)

Zhao C.-M. et al.: Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach. Cell Tissue Res. 290, 539-551 (1997)

For Research Use Only

See also guinea pig anti-histidine decarboxylase $562.00/50ul


Guinea Pig Polyclonal Antibody to Histidine Decarboxylase

cat$RDI-PRO16046   $688.00/50ul

Category                      Guinea pig polyclonal

Form/Purification        Undiluted guinea pig serum (lyoph.)

Immunogen                   Recombinant histidine decarboxylase produced in E.coli

Description/Specificity Histidine decarboxylase is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in the mast cells.

Positive Control Stefanini-fixed frozen sections of rat fundus

Species tested so far             Human, rat, mouse, dog, frog and chicken

Application · Immunohistochemistry, immunofluorescence microscopy

                     · Suitable for paraffin and frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:800 – 1:1200 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

References

Dartsch C. et al.: Recombinant expression of rat histidine decarboxylase: generation of antibodies useful for Western blotanalysis. Int. J. Biochem.& Cell. Biol. 30, 773-782 (1998).

Zhao C.-M. et al.: Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach. Cell Tissue Res. 290, 539-551 (1997).


need a secondary antibody?
RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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