rev: October 22, 2002

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Glutamic Acid Decarboxylase  GAD


cat#RDI-PRO16036 sold out, see monoclonals below as substitute
Mouse Anti-Glutamic acid decarboxylase (GAD)   Human/Mouse

cat# RDI-GADabm-9A6   $500.00

Presentation: 100ug lyophilized. Reconstitute with 100ul DI water to yield a 1.0 mg/ml stock in PBS (pH7.2) with 1% sucrose & 0.1% Sodium azide. *When disposing azide into a drain, flush with plenty of water.

Clone: 9A6

Isotype: Mouse IgG1

Derivation: purified from mouse ascites fluid using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma P3U1 with Balb/c splenocyte immunized with  -human brain GAD 65 fusion protein corresponding to full length amino acids (1-585 a.a.).

SPECIFICITY : reacts with GAD 65 and GAD 67 by Western blotting (approx 1:1000) and immunohistochemistry (1- 10ug/ml) staining. This antibody detects 65KD and 67KD of human and mouse GAD by Western blotting on total cell lysate from mouse brain. Not tested with other species -Positive control include human and mouse brain lysates.

STORAGE : Store lyophilzed antibody at 4 DEG C on arrival. After reconstitution, store 4 DEG C short term or recommend aliquoting and store at -20 DEG C in non-frost free freezers. Avoid frequent freeze thaw cycles.

BACKGROUND : Glutamic acid decarboxylase (GAD) catalyzes formation of gamma -Aminobutyric acid (GABA), which is the inhibitory neurotransmitter, and is present in brain as well as several tissues outside the central nervous system. Biological functions of GAD and GABA extend beyond regulation of neurotransmission to include effects on the immune system as well as modulation of cell proliferation, protein synthesis, and metabolism 1) . Two isoforms of GAD, GAD 65 and GAD 67 each derives from a single separate gene, were isolated from human fetal brain lambda gt11 cDNA library 2)

REFERENCE:

1) Karlsen A. E. et al Proc. Natl. Acad. Sci. 88 ,8337 (1991)

2) Michelsen B. K. et al. Proc. Natl. Acad. Sci. 88, 8754 (1991)

3) Bu D. F. et al. Proc. Natl. Acad. Sci. 89 , 2115 (1992)

4) Deaizpurua H. J. et al. Diabetes 41 , 1182 (1992)

5) Mauch L. et al. J. Biochem. 113 , 699 (1993)

6) Grubin C. E. et al. Diabetologia 37 , 344 (1994)

7) Daw K. et al. J. Immunol. 156 , 818 (1996)

8) Kasuga A. et al. J. Autoimmunity 9 , 105 (1996)

For Research Use Only


Mouse anti-GAD65( Human/Mouse/rat/Pig)

cat# RDI-GADabm-6   $500.00

Presentation: 100ug in 0.2ml (0.5mg/ml) PBs pH7.2 with 0.09% sodium azide

Clone: GAD-6

Isotype: mIgG2a

Immunogen: purified GAD65 from rat brain

Epitope: region within last 41 amino acids of GAD 65

Use: -Western blot: approx 0.5-1ug/ml using rat brain cortex as positive control, band 65kda

        -immunoprecipiation

       -histochemistry (aceotne fixed frozen sections, 5- 10ug/ml)

Storage: 4 DEG C

Ref: -Diabetes 42:1799-1808 (1993)

       -J Biol Chem 274:24366-24371 (1999)

        -J Neurosci 8:2123-30 (1988)

Background: gamma-aminobutyric acid (GABA) is an amino acid neurotransmitter that is considered to be the major inhibitory neurotransmitter in the mammalian central nervous system. The highest concentrations of GABA are found in the brain, where it is synthesized from glutamic acid to GABA by an enzyme called glutamic acid decarboxylase (GAD). GAD is also expressed in the insulin-producing â cells of the islets of Langerhans. Two isoforms of GAD are present in rat brain, GAD65 and GAD67, based on their relative molecular weight in kDa. Both isoforms have significant levels of homology in the catalytic portion of the molecule, but differ greatly in the first 95 amino acids in the N-terminal region. GAD65 migrates at ~65 kDa in SDS/PAGE.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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