rev: January 11, 1999
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Category Rabbit polyclonal
Form/Purification Undiluted rabbit serum (lyoph.)
Immunogen Synthetic met-enkephalin (Sigma), conjugated to BSA
Description/Specificity Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while ß-endorphin does not.
Positive Control Bouin-fixed paraffin sections of cat ileum.
Species tested so far Pig, cat
Application · Immunohistochemistry, Immunofluorescence
· Suitable for frozen and paraffin sections
Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.
Working Dilution 1:400 - 1:800 (PAP)
1:200 - 1:400 (immunofluorescence microscopy) with overnight incubation at 2-8°C.
Storage At 2-8°C (lyoph.) or at -20°C (aliquots)
Quantity / Volume 50 µl (lyoph.)
Thesleff P et al. A mixed endocrine adrenal tumor causing steatorrhea. Gut 28(10), 1298-1301 (1987)
For Research Use Only
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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