rev: January 11, 1999

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Enkephalin (polyclonal)
Polyclonal Antibody to Enkephalin

Cat#RDI-PRO16020    $406.00

Category Rabbit polyclonal

Form/Purification Undiluted rabbit serum (lyoph.)

Immunogen Synthetic met-enkephalin (Sigma), conjugated to BSA

Description/Specificity Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while ß-endorphin does not.

Positive Control        Bouin-fixed paraffin sections of cat ileum.

Species tested so far      Pig, cat

Application · Immunohistochemistry, Immunofluorescence

                  · Suitable for frozen and paraffin sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:400 - 1:800 (PAP)

                            1:200 - 1:400 (immunofluorescence microscopy) with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference

Thesleff P et al. A mixed endocrine adrenal tumor causing steatorrhea. Gut 28(10), 1298-1301 (1987)

For Research Use Only


need a secondary antibody? see http://www.researchd.com/rdiabs/2ndabs.htm
RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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