rev: October 26, 1998
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Category Rabbit polyclonal
Purification/Form Rabbit serum
Immunogen Synthetic bombesin conjugated to bovine thyroglobulin
Description/Specificity This polyclonal antibody can be used for the study of endocrine differentiation in tumors of the respiratory tract.
Recommended Positive Control Gut (peripheral nerves), fetal lung
Antigen Recognized Human (also reported mouse, rat and frog)
Application Immunohistochemistry Suitable for frozen and paraffin section
Proteolytic treatment recommended (see below)
Working Dilution 1:50 for immunohistochemistry
Dilution Buffer PBS with 1% albumin and 0.1% Na-Azide
Incubation Time 30 min at room temperature or overnight at 2-8°C
Storage At 2-8°C (undiluted) or at -20°C (aliquots)
Volume 0.25 ml
Protocol for the use of anti-Bombesin polyclonal antibody on paraffin-embedded tissue sections:
Small tissue blocks are fixed in 4% buffered formaldehyde (pH 7.6) at 4°C. The fixation time should not exceed 12 h.
During the embedding process temperatures should not exceed 58°C.
Deparaffinize and rehydrate sections.
If necessary, block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol, 15 min). Rinse in PBS 3x.
Incubate for 5 min in 0.1% pronase in PBS at 37°C. Rinse in PBS 3x.
Apply properly diluted anti-Bombesin antibody (1:50) and incubate for 30 min at room temperature or overnight at 4°C. Rinse 3x.
Apply conjugated antibody. Rinse in PBS 3x.
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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