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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat#RDI-PRO10704       $438.00

Clone               6F9

Category          Mouse monoclonal, purified IgG

IgG Class         IgG1

Antigen            Human uvomorulin

Description/Specificity In epithelia, E-Cadherin is involved in the development and maintenance of cell layers during embryogenesis and in the adult tissue. Clustering of E-Cadherin seems to occur via a, ß and g catenins which link E-Cadherin to cytoskeletal actin. The E-Cadherin-catenin complex formation is crucial for cell adhesiveness. Loss of E-Cadherin expression seems to be correlated with the transformation state of carcinoma cells and with their metastatic potential. The antibody reacts specifically with the 120 and 80 kD ARC-1 protein in Western blotting.

Recommended Positive Control   Cell line MCF-7,  human small intestine

Application :    ELISA Immunoblotting  Immunohistochemistry

                       Suitable for frozen sections (recommend clone 5H9 for paraffin cat#RDI-PRO10028

Antigen Recognized :human

Application         ELISA

                            Immunoblotting

                           Immunohistochemistry

                          Suitable for frozen sections

Antigen Recognized         human

in Species (tested so far)

Working Dilution      1:10 - 1:40 (for immunohistochemistry)

Dilution Buffer         Dilute immediately before use with PBS containing 1% BSA and 0.09% Na-Azide

Incubation Time       30 min at room temperature/overnight at 2-8°C

Storage                    At 2-8°C (undiluted) or at -20°C (aliquots)

Volume                    1 ml

References:

Frixen, U.H., et al. E-Cadherin-mediated Cell-Cell Adhesion Prevents Invasiveness of Human Carcinoma Cells (1991). J. Cell Biol. 113, 173-185.

Umbras R., Schalken J.A., Aalders T.W., Carter B.S.. Karthaus H.F.M., Schaafsma H.E., Debruyne F.M.J., Isaacs W.B. Expression of the Cellular Adhesion Molecule E-Cadherin is Reduced or Absent in High-Grade Prostate Cancer. Cancer Research (1992), 52, 5104-5109.

Mayer B. et al.: E-Cadherin expression in primary and metastatic gastric cancer: Down-regulation correlates with cellular dedifferentiation and glandular disintegration (1993). Cancer Research 53, 1690-1695.

Moll R. et al.: Differential loss of E-Cadherin expression in infiltrating ductal and lobular breast carcinomas (1993). Am. J. Pathol. 143, 1731-1742.

For In Vitro Research UseOnly

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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