rev:    June 3, 2004

HOME (index page)

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see also other tubulin antibodies  , and beta III isoform
Mab to Tubulin

cat#RDI-PRO10610     $406.00/vial

Clone 655

Category Mouse monoclonal

Form/Purification Ascites

Ig Class IgM

Immunogen Isolated from chicken muscle cells

Clinical Application/Specificity Tubulin is found in the microtubules which represents the main structural components of centriols, the mititic spindle , cilia and flagella. Mab 655 specifically stains tubulin in immuno-histochemistry and reacts in ELISA and WB. The antibody cross reacts with human tissue.

Antigen Recognized in Species (tested so far)  :  Human, chicken

Positive Control :   Cultured fibroblasts

Suitable for · Immunohistochemistry

                   · Immunofluorescence

                   · Immunoblotting

                   · ELISA

Dilution Buffer TRIS buffer with 1% BSA and 0.09% Na-Azide


Working Dilution 1:5 - 1:20


Storage At 4°C (undiluted)


Volume 1 ml


Reference :Bläckström B and Collins VP: Cytoskeletal changes in axons of rats exposed to 2,5-hexamediol, demonstrated using monoclonal antibodies. Neurotoxicology Vol. 8, 1:85-96 (1987)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-Alpha Tubulin (Multi-Species)   Paraffin Sections/Western Blot

cat# RDI-TUBULAAbm-DM       $438.00/100ug

       RDI-TUBULAabm-DM1       $688.00/200ug


Presentation: purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml)

-also available azide/BSA free   cat#RDI-TUBULAabm-DMX  $750.00/200ug/0.2ml



Clone ref: DM1A

Isotype: mIgG1a

Immunogen: Native chick brain microtubules

Hybridoma: fusion between BALB/c57 splenocytes and myeloma NS-1 cells.

Species Reactivity: Human, Cow, Pig, Rat,Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, and Fungi.

                                Others-not tested.



Use: -western blot (1ug/ml for 2 hours RT)

         -histochemsitry: frozen sections and formalin fixed paraffin sections (no special pretreatment required)

          -electron microscopy & immunofluorescence

Positive Control: LS174T, MAD109 cells. Skin or lung

Localization: cytoplasmic

Specificity: Recognizes a protein of 57kDa, identified as tubulin-alpha. This Mab is highly specific to alpha tubulin and shows no cross-reaction with Beta tubulin . Its epitope localizes between aa 426-450; aa 426-430 are critical for reactivity.2 . This Mab causes the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Mabdoes not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

Key References:

1. Blose SH, et. al. Journal of Cell Biology, 1984, 98(3):847-58.

2. Breitling F, et. al. Journal of Molecular Biology, 1986, 189(2):367-70.

3. Biochim Biophys Acta, 1989, 1012(1):46-56.

4. Biochemistry, 1993, 32(18):4955-61.

For Research Use Only


DATA SHEET: (mouse anti-ALPHA Tubulin )

Catalog#: RDI-TUBULAabm    $531.00

Package Size: 0.5ml

Clone Ref: B-5-1-2

Ig Isotype: mouse IgG1

Antigen: Sarkosyl-resistant filaments from sea urchin (Stronglocentrotus purpuratus) sperm axonemes


Reactivity: reacts with an epitope located in the C- terminal end of the alpha tubulin in a many species inclduing human, sea urchin and Chlamyodomas. Reactivity has also been seen in human, mouse, rat, African Green monkey, kangaroo rat, chicken, sea urchin and Chlamydomonas.


Use: immunoblotting, immunocytochemistry using tissues or cell cultured cell line preparations, indirect immunofluorescence of   human cultured fibroblasts ( 1:2000 dilution).

-Titer for each use


Storage: Store at -20 DEG C Upon receipt Avoid repeated freeze thaw cycles.


ref: J. Cell Biol, 104, 289 (1987)

      Enzymol, 196 , 264 (1991)

      Natl Acad Sci USA, 84, 5720 (1987)

      J. Cell Biol, 106, 1213 (1988)


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


Mouse anti-Beta Tubulin (Multi-Species)   Paraffin Sections/Western Blot

cat# RDI-TUBULBAbm-5      $438.00/100ug

       RDI-TUBULBabm-51    $688.00/200ug

Presentation: purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml) -also available azide/BSA free cat#RDI-TUBULBabm-5X $750.00/200ug

Clone ref: TBN05 (also known as TUb 2.1)

Isotype: mIgG1

Immunogen: purified tubulin

Epitope: C-terminal

Species Reactivity: Human, Cow, Rabbit,Mouse, Chicken, Rat, Frog, Moth, and Wheat. Others not known.

Localization: Cytoplasmic

Use: -western blot (0.5ug/ml for 2 hours RT)

-histochemistry: frozen sections and formalin fixed paraffin sections (no special pretreatment required) 1-2ug/ml

-electron microscopy & immunofluorescence

Positive Control: Skin or lung, MAD109cells or Ls174T

Specificity: Recognizes a protein of 55kDa, identified as tubulin Beta.

Key References: 1. Gozes, I., and Barnstable, C.J., Proc. Natl. Acad. Sci. USA, 79, 2579 (1982).

                            2. Matthes, T., et al., J. Immunol., 141, 3135 (1988).

For Research Use Only


Rabbit anti-Beta Tubulin (Multi-Species) Paraffin Sections/Western Blot

cat# RDI-TUBULBabR    $438.00/100ug

        RDI-TUBULBabR1  $688.00/200ug

Presentation: purified rabbit polyclonal antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml)

Immunogen: synthetic peptide derived from aa 416-430 of human tubulin beta

Species Reactivity: Human, Cow, Pig, Rat,Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, and Fungi. sea urchin, myxamoeba and plasmodium.Others-not tested.

Localization: Cytoplasmic

Use: -western blot (1ug/ml for 2 hours RT)

       -histochemistry: frozen sections and formalin fixed paraffin sections (no special pretreatment required) 1-2ug/ml

Positive Control: Skin or lung

Specificity: Recognizes a protein of 55kDa, identified as tubulin Beta. Its epitope localizes between aa 416-430. although not tested for this activity, a similar Monoclonal ab clone DM1B (no longer available) did not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

For Research Use Only


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)

Ordering terms