rev: May 18, 2004
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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mab to Desmoglein 3
Category Mouse monoclonal
Immunoglobulin Class IgG1
Form/Purification Hybridoma culture supernatant
Antigen Recombinant human polypeptide Desmoglein 3.
Description/Specificity In immunoblots of cytoskeletal preparations from human epidermis the antibody reacts specifically with a 140 kD polypeptide typical for Desmoglein 3. In immunolocalization studies the mab shows typical staining of basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus vulgaris patient sera. Cross-reaction with Desmogleins 1 and 2 was not detected.
Antigen Recognized in Species Human
(tested so far)
Application Suitable for · immunohistochemistry on sections
· immunoblotting (Western)
better resolution on frozen sections preincubation (directly after fixation)
with 0.05 - 0.2% Triton X-100 for 5 -10 min, depending on tissue type, is
recommended (ref. 2).
Working Dilution Ready-to-use for immunohistochemistry
Incubation Time 1 h at RT for immunohistochemistry
Storage At 2-8°C
Quantity 5 ml (contains 0.09% NaN3)
1.Kurzen H, Moll I, Moll R, Schäfer S, Simics E, Masayuki A, Wheelock MJ, Franke WW. Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation 63, 295-304 (1998)
2.Schäfer S., Stumpp S., Franke W.W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation 60, 99-108 (1996)
For In Vitro Research Use Only
San Jose, 95123 CA Snell ave 658
or (800) 370-2222
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