rev:   February 28, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Peroxisome Proliferation Activated Receptors Antisera (PPAr)

 see also new PPAR-A phospho abs


Monoclonal (Mouse) Anti-PPAR-Alpha Antibody   (Human/Mouse/Rat)

cat# RDI-PPARAabm-22    $469.00

Presentation: 100ug in 0.1ml PBS with 1mg/ml BSA and 0.05% sodium azide

Clone: 3B6/PPAR,

Isotype: IgG2b

Derivation: immunizing BALB/c mice with purified recombinant PPARa protein.

Uses: -has been successfully used in Western blot and immunoprecipitation procedures to detect PPARa protein from human, mouse, and rat sources. By Western analysis this antibody detects a ~52 kDa protein which corresponds to PPARa.

      -Western Blot 2-5 ug/ml

      -Immunoprecipitation ( Assay Dependent)

     *Various assay conditions require that the optimal working concentrations be determined by serial

Storage: Store at -20oC. Avoid repeated freeze/thaw cycles.

references: R.M. Lavinsky et al. Diverse signaling pathways modulate nuclear receptor recruitment of N-CoR and SMRT complexes. Proc. Natl. Acad. Sci. 95: 2920-2925, 1998.

M.W. Kilgore et al., MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol. and Cell. Endo. 129: 229-235, 1997.

R. A. Roberts, et al. Evidence for the Suppressin of Apoptosis by the Peroxisome Proliferator Activated Receptor a (PPARa). Carcinogenesis 19:43-48, 1998. dilution ofthis product.

Background: Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. PPARa is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPARa is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the b-oxidation of fatty acids. Activation of rat liver PPARa has been shown to supress hepatocyte apoptosis. PPARa, like several other nuclear hormone receptors, heterodimerizes with RXRa to form a transcriptionally competent complex.

For Research Use Only


rev:October 8, 2001

Polyclonal (Rabbit) Anti-Peroxisome Proliferator Activated   Receptor a Antibody (Human/Mouse)

Cat# RDI-PPARAabrx   $470.00
blocking pepitde cat#RDI-PPARAA-CP $188.00/50ug

Presentation: 100 ul of epitope affinity purified rabbit IgG with 1.0 mg/ml BSA and 0.05% Sodium Azide .

Derivation: -immunizing New Zealand white rabbits with an 18 residue synthetic peptide whose sequence is derived from murine PPARa and is completely unique to PPARa. A single amino acid substitution (I8 to L8) exists between the murine and human protein. This product exhibits no cross reactivity with PPARd or PPARg. A COOH-terminal cysteine residue was added for conjugation to KLH.

Peptide Sequence: M1 V D T E S P I C P L S P L E A D D18 C

Use: Western Blot 1:500

          Immunohistochemistry 1:500

          Gel Supershift 1-5 ul *Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product.

-has been successfully used in Western blot, gel shift, and immunohistochemical procedures to detect PPARa protein. By Western analysis of various mouse tissue extracts this antibody detects a ~52 kDa protein which corresponds to PPARa. In gel shift experiments this product inhibits PPARa DNA binding.

Storage: Store at -20 DEG C. Avoid repeated freeze/thaw cycles.

Ref: Carcinogenesis, 19: 43-48, 1998.

       Gasto., 124:184-201, 2003.

        Mol. And Cell. Endo., 129: 229-235, 1997.

Background:

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specificresponse elements. PPARa is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPARa is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the b-oxidation of fatty acids. Activation of rat liver PPARa has been shown to supress hepatocyte apoptosis. PPARa, like several other nuclear hormone receptors, heterodimerizes with RXRa to form a transcriptionally competent complex.

For Research Use Only

cat#RDI-PPAA-CP 50 µg lyophilized, >95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.  blocking peptide: is an 18 amino acid (amino acids 1-18) synthetic peptide whose sequence is derived from murine PPARa and is completely unique to PPARa. A COOH-terminal cysteine residue was added for conjugation to KLH. The sequence of this peptide is (amino to carboxy terminus):

M1 - V - D - T - E - S - P - I - C - P - L - S - P - L - E - A - D - D18 - C

This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and PPARa, catalog #RDI-PPARAabrx. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.


Polyclonal (Rabbit) Anti-Phosph-PPAR-Alpha (pSer21)   Peroxisome Proliferator Activated Receptor A Antibody   (Mouse)

Cat# RDI-PPARA21abrx   $500.00

blocking peptide cat#RDI-PPARA21-CP   $188.00/50ug

Presentation: 100 ug in 0.1ml epitope affinity purified rabbit IgG with 1.0 mg/ml BSA and 0.05% Sodium Azide .

Derivation: -immunizing New Zealand white rabbits with an 21 residue synthetic peptide (aa14-25) whose sequence is derived from murine PPARA

Peptide Sequence: (14) L E A D D L E pS) P L S E (25)

Use: Western Blot 2ug/ml

*Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product. -By Western analysis of various mouse tissue extracts this antibody detects a ~52 kDa protein which corresponds to phospho-PPARa (pos control mouse adipose tissue extract)

Storage: Store at -20 DEG C. Avoid repeated freeze/thaw cycles.

Ref: Mol. And Cell. Endo., 129: 229-235, 1997.

Proec Natl Acad Sci 95:2920-2925, 1998

Background:

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. PPARa is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPARa is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the b-oxidation of fatty acids. Activation of rat liver PPARa has been shown to supress hepatocyte apoptosis. PPARa, like several other nuclear hormone receptors, heterodimerizes with RXRa to form a transcriptionally competent complex.

For Research Use Only

cat#RDI-PPARA21-CP 50 µg lyophilized, >95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.  blocking peptide:

This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and PPARa, catalog #RDI-PPARA21abrx. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.


Polyclonal (Rabbit) Anti-Phosph-PPAR-Alpha (pSer12)   Peroxisome Proliferator Activated Receptor A Antibody (Mouse)

Cat# RDI-PPARA12abrx    $500.00

blocking peptide cat#RDI-PPARA12-CP $188.00/50ug

Presentation: 100 ug in 0.1ml epitope affinity purified rabbit IgG with 1.0 mg/ml BSA and 0.05% Sodium Azide .

Derivation: -immunizing New Zealand white rabbits with an 12 residue synthetic peptide (aa8-19) whose sequence is derived from murine PPARA

Peptide Sequence: I(8) C P L (pS) P L E A D D L (19)

Use: Western Blot 2ug/ml

*Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product. -By Western analysis of various mouse tissue extracts this antibody detects a ~52 kDa protein which corresponds to phospho-PPARa (pos control mouse adipose tissue extract)

Storage: Store at -20 DEG C. Avoid repeated freeze/thaw cycles.

Ref: Mol. And Cell. Endo., 129: 229-235, 1997.

Proec Natl Acad Sci 95:2920-2925, 1998

Background:

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. PPARa is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPARa is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the b-oxidation of fatty acids. Activation of rat liver PPARa has been shown to supress hepatocyte apoptosis. PPARa, like several other nuclear hormone receptors, heterodimerizes with RXRa to form a transcriptionally competent complex.

For Research Use Only

cat#RDI-PPARA12-CP   50 µg lyophilized, >95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.  blocking peptide:

This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and PPARa, catalog #RDI-PPARA12abrx. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.


Polyclonal (Rabbit) Anti-PPAR Antibody (BROAD)    Human/Mouse/Rat

Cat# RDI-PPARabrx    $470.00

Presentation: 100 ul of diluted rabbit antiserum.

Derivation: -immunizing New Zealand white rabbits with a 15 amino acid residue synthetic peptide (below) whose sequence is derived from the C-terminal region of PPARg2 which is highly conserved in mouse, human and rat PPARg1, PPARa and NUC1.

Peptide sequence: I (484) K K T E T D M S L H P L L Q (498)

Use: -has been successfully used in Western blot and gel super shift experiments to detect the presence of PPARg2 in mouse 3T3L1 cells. It has also been shown to react with recombinant rat PPARa by Western blot and recombinant mouse PPARg1 by immunoprecipitation.

Dilution: Western blot 1: 2000

                Immunolocalization ND

                 Immunoprecipitation Assay dependent

                Gel shift assay Assay dependent

              *Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product.

Storage: Avoid repeated freeze/thaw cycles.

Specificity

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with RXRa.

For Research Use Only


Polyclonal (Rabbit) Anti-PPAR Gamma Antibody   Human/Mouse/rat)

Cat# RDI-PPARGabrx   $469.00

Presentation: 100 ul of diluted rabbit antiserum with 0.05% sodium   azide

Derivation: -produced by immunizing New Zealand white rabbits with a 15 residue synthetic peptide (below) whose sequence is derived from mouse PPARg2 and is completely conserved in PPARg1, but exhibits no significant homology with PPARa or NUC1. The sequence is completely conserved in mouse, human and rat

Peptide : M(284) M G E D K I K F K H I T P L (298)

USE: has been successfully used in Western blot & gel super shift procedures to detect the adipose specific PPARg2 isoform in mouse 3T3-L1 cells.

Dilution: Western blot 1: 2000

Gel shift assay Assay dependent

*Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product.

Storage: -20 DEG C. Avoid frequent freeze thaw cycles

Specificity

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. The PPARg2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR g2, like several other nuclear hormone receptors, heterodimerizes with RXRa.

Additional References

M.W. Kilgore et al., MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol. and Cel. Endo., 129: 229-235, 1997.

R.M. Lavinsky et al. Diverse signaling pathways modulate nuclear receptor recruitment of N-cor and SMRT complexes. Proc. Natl. Acad. Sci., 95: 2920-2925,

1998. For Research Use Only


Polyclonal (Rabbit) Anti-PPAR-Gamma2 Antibody   (Human/Mouse)

Cat# RDI-PPARG2abrx   $469.00

Presentation: 100 µl of epitope affinity purified rabbit IgG with 1.0   mg/ml BSA in 100 ul of PBS with 0.05% sodium azide

Derivation: -produced by immunizing New Zealand white rabbits with a 16 residue synthetic peptide (below) whose sequence is   derived from mouse PPARg 2 and is absent in PPARg 1. This product exhibits no cross reactivity with PPARa or PPARd.   A COOH-terminal cysteine residue was added for   conjugation to KLH. M1 G E T L G D S P I D P E S D S16C   The immunizing peptide (Cat#RDI-PPARG2-CP $125.00) is available for use in neutralization and control   experiments.

Use: -has been successfully used in Western blot, gel shift, and immunoflourescence procedures to detect PPARg2 protein. By Western analysis of various mouse tissue extracts this antibody detects a ~56 kDa protein which corresponds to PPARg2. In gel shift experiments this product inhibits PPARg2 DNA binding.

Dilution: Western Blot 1:500

                Immunoflourescence 1:500

                Gel Supershift 1-5 ul

               *Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product.

Storage: -20 DEG C , Avoid frequent freeze thaw cycles

Specificity

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. The PPARg2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPARg2, like several other nuclear hormone receptors, heterodimerizes

with RXRa. For Research Use Only


Polyclonal (Rabbit) Anti-Peroxisome Proliferator   Activated Receptor DELTA Antibody

Cat# RDI-PPARDabrx    $470.00

-blocking peptide cat#RDI-PPARD-CP  $156.00/50ug

Presentation: 100 ul of epitope affinity purified rabbit IgG with 1.0 mg/ml BSA and Sodium Azide 0.05%. Store at -20oC. Avoid repeated freeze/thaw cycles.

Derivation: -produced by immunizing New Zealand white rabbits with a 14 residue synthetic peptide whose sequence is derived from murine PPARd and is completely unique to PPARd. This sequence is 86% conserved in human PPARd. This product exhibits no cross reactivity with PPARa or PPARg. A COOH-terminal cysteine residue was added for conjugation to KLH. Sequence: M1 E Q P Q E E T P E A R E E14 C

Use: -has been successfully used in Western blot, gel shift, and immunohistochemical procedures to detect PPARd protein. By Western analysis of various mouse tissue extracts this antibody detects a ~49 kDa protein which corresponds to PPARd.

Dilution: Western Blot 1:500

                Immunohistochemistry 1:500

               Gel Supershift 1-5 ul

              *Various assay conditions require that the optimal working concentrations be determined by serial dilution of this product.

Specificity

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR’s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR proteins can induce transcription of acyl coenzyme A oxidase & CYP450 A6 through interaction with specific response elements. The PPARd isoform has also been refered to as NUC1, PPARb, and FAAR in the literature. PPARd is expressed in nearly all tissues which suggests this isoform may play a general ‘housekeeping’ role. PPARd like several other nuclear hormone receptors, heterodimerizes with RXRa to form a transcriptionally competent complex.

For Research Use Only


All antibodies are for In Vitro Research Use Only-Not for Use in Diagnostics

-copyright by owner


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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