rev: March 18, 2002

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(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Other Phosphorylation Antibodies: (BULK QUOTES ON REQUEST)

#RDI-PHOSTYRabr   rabbit anti-Phosphotyrosine     100ug $190.00

#RDI-PHOSSERabr   rabbit anti-Phosphoserine        50ug $190.00

#RDI-PHOSTHRabr   rabbit anti-phosphothreonine   50ug $190.00

#RDI-PHOSTPabr      rabbit anti-Phosphothreonine-Proline 50ug   $188.00

#RDI-PHOSTLYTabr  rabbit anti-Phosphothreonine-Lysine-phosphotyrosine 50ug  $190.00

Product: Affinity Isolated Rabbit Anti-Phosphothreonine-any-Phostyrosine Antibodies (anti-pT-X-PY)

Catalog:  RDI-PHOSLYTabr   $188.00/50ug vial  (Bulk quotes on request)

Background: Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.

Immunogen: KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugates

Purification: purified with N-acetyl-phosthreonine-lysine-phostyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose.

Presentation: 50ug in 0.2ml (0.25mg/ml) in PBS with 0.02% NaN3

Storage: at 2-8oC, do not freeze

Specificity Reactivity: In Elisa, specifically reacts with peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacts to pT-P-Py sequence. The antibody does not react with phosphothreonine and phosphoserine but weakly to phosphotyrosine under optimum conditions.

Applications: -ELISA (kinase assay), 0.5 ug/mL;

                      -Western blot, 1ug/mL (colorimetric), 0.5ug/ml or less with chemiluminescence;

Positive Control: Mouse brain extract for immunoblotting. Synthetic pT-X-pY motif for Elisa.

Note: For research use only.

For In Vitro Research Use Only-Not For Use IN Or On Humans or Animals-Not For Use in Diagnostics.

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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266