rev: October 9, 2000

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PHOSPHOLAMBAN  (see also phospho-phospholamban below)

-phospholamban is a pentameric sacroplasmic reticulum membrane protein. It has intrinsic inhibitory activity towards SR Ca+ ATPase, which is alleviated by hyperphosphorylation of phospholamban. Kinases capable of phospholamban phosphorylation include PKA and CaMKII. Phospholamban is hypophosphorylated in ischemic heart, and hyperphosphorylated in cornoary arteries induced to relax by NO. Given its localization to the SR, phospholamban makes a suitable marker of the SR.


DATA SHEET: Mouse anti-Phospholamban

(mouse,rat, porcine, bovine and canine)

Catalog#: RDI-PHOSLAMBabm

Price: $562.00/vial   $500.00/vial 3 or more

Package Size: 50 micrograms

Species: mouse IgG1 purified by column chromatography

Antigen: canine cardiac phospholamban

Activity: Western blot: 0.5 micrograms/ml (25 kda)

                immunoprecipitation:4ug

                cytochemistry: 10ug/ml

                ELISA: 0.05ug/well

                 biological: stimulates activity

               -MUST TITER FOR EACH APPLICATION

ref: J. Biol Chem 261:7018, 1986

     Cell Biol. 65:302, 1987

     Biochemsitry 29:4535, 1990

General ref: S. Minimisawa et al, "Chronic phospholamban-sacroplasmic reticulum calcium ATPase interaction is the critical calcium cycling defect in dilated cardiomyopathy" , Cell, 99:531-41

Storage: Upon receipt, store at -20 DEG C or below. -aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


DATA SHEET: Rabbit anti-PHOSPHO-Phospholamban (Ser16)

(human,mouse,rat, porcine, bovine and canine)

Catalog#: RDI-PPHOSLAMBabR

Price: $500.00/vial    $469.00/vial 3 or more

Package Size: 100 micrograms in 0.238ml 0.1M Tris-glycine, pH7.4, 0.15M NaCl with 0.05% sodium azide with 30% glycerol

Species: rabbit IgG purified by column chromatography

Antigen: synthetic peptide corresponding to amino acids 14-25 derived from human cardiac phospholamban with an added C-terminal cysteine conjugated to KLH. The sequence is identical in mouse, rat, rabbit, pig and dog. (Ra(ps)TIEMPQQAR-C)

Specificity: recognizes phosphorylated phospholamban (SER16), mw 5-25kda.

Species Reactivity: bovine (predicted with human, mouse,rat, rabbit, pig and dog)

Activity: Western blot: 0.5-2 micrograms/ml (5-25 kda)

-positive control:bovine cardiac preparation treated with 10ug PKA for 10 minutes

-MUST TITER FOR EACH APPLICATION

ref: Biochem Biophys Research Comm 267:40-48,2000

Storage: Upon receipt, store at -20 DEG C or below. -aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for use in or onhumans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

Upon first thaw or on receipt, tighten cap, centrifuge at 1000 rpm for 1-2 minutes to concentrate antibody in bottom of vial.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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