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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-PAXILabm $438.00/vial 50ug
RDI-PAXILabm-1 $750.00/vial 150ug
Background: Paxillin is a focal adhesion protein and a substrate for several
tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation
of paxillin is affected by conditions that change cell-cell adhesion, consistent
with the belief that paxillin is involved in the regulation of cell morphology.
Additionally because of is SH3-binding domain, paxillin associates tightly
with FAK and Crk in a manner that is independent of the extracellular matrix.
Although paxillin was initially detected in fibroblasts, its phosphorylation
may also be important during neurite extension during differentiation.
Antigen: derived from chick paxillin
package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a
50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM
Use: Suitable for western blot on human, rat, mouse, dog and chick using
approx 0.5 ug/ml ab on total cell lysate using human endothelial cells as
positive control. Major band at Mw 68kDa. Recommend 2-3 hour RT or overnight
4 DEG C incubation with enhanced chemiluminescence detection. Also suitable
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.
Main REF: J. Biol Chem 1995, 270:5039
J. Biol Chem, 1996, 271:5957
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
-copyright by owner
FITC conjugated cat#RDI-PAXIL-49FT $438.00/50ug or cat#RDI-PAXIL-49FT1 $875.00/150ug
TRITC conjugated cat#RDI-PAXIL-49TC $438.00/50ug or cat#RDI-PAXIL-49TC1 $875.00/150ug
cat# RDI-PAXIL-5H11 $438.00/100ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration
Immunogen: purified recombinant full length human paxillin protein
Epitope: not mapped
Reactivity: human and rat (others not tested)
Uses: -Western blot: 1ug/ml for 2 hours rt
-histochemistry (paraffin sections) approx 2-4ug/ml for 60 minutes RT
-for paraffin sections, requires boiling tissue sections in 1mM EDTA pH8.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
-IMMUNOPRECIPITATION: approx 2ug/mg protein lysate use Protein G under native conditions
Positive Contrl:HELA cells or colon carcinoma
Localization: cell membrane and cytoplasmic
Background: Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
Storage: 2-8 deg c
For Research Use Only
RDI Div of researchd Industries Intl
(Research Diagnostics Inc)
San Jose, 95123 CA Snell ave 658
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
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