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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PARP (POLY ADP-RIBOSE Polymerase) Antibodies

Rabbit anti-PARP (Human/Mouse /Rat)

cat#RDI-PARPabr   $562.00/200ug in Tris-HCL/glycerol + 0.05% NaN3

-Rabbit polyclonal

-antigen:synthetic peptide G V D E V A K K K C: corrresponding to the region downstream of the YAMA cleavage site of human PARP with an added cysteine for conjugation to KLH

-specific for PARP

-human, mouse and rat reactive

-suitable for western blotting (0.5-2ug/ml band approx 116kDa and 85kDa fragment, using control lysates of HeLa nuclear extract, with addtional band at <66kDa if 4M Urea not used in the cell lysis buffer) immunoprecipitation (4ug)


Rabbit anti-PARP (Mouse /Rat/Bovine/Primate...)

cat#RDI-PARPabrX   $469.00/100ul diluted rabbit sera + 0.05% NaN3

-Rabbit polyclonal

-Immunogen: synthetic peptide conjugated to carrier protein   corresponding to amino acid residues 509-524 in the automodification domain of Bovine PARP.

-sequence: S509-K-G-P-V-K-E-E-G-T-N-K-S-E-K-R

-specific for PARP

-mouse , rat, bovine, and primate reactive

-suitable for western blotting (1:500 dilution band approx 116kDa and 85kDa fragment) and ELISA


Sample DATA SHEET:Mouse anti-human PARP monoclonal

Catalog#: RDI-PARPabm-X    Price: $438.00/vial

Package Size: 100ug in 1ml PBS with 0.1% NaN and 0.2% BSA.

Ig Isotype: mouse IgG1

clone: C-2-10

Antigen: purified calf thymus poly (ADP-ribose) polymerase.

Epitope: C terminus of PARP DNA-binding domain

Reactivity: -Binds to human, monkey, hamster, rat and mouse (not with chicken)

-Suitable for: western blotting (1ug/ml)using chemiluminescence detection

-This antibody recognizes full length (116kda PARP and the 85 kDa apoptosis-related cleavage fragment of PARP and may be used to stain cytospin preparations of HL-60 cells treated with etoposide (suggested antibody starting concentration, 2ug/ml. Antibody must be titrated for optimal staining in individual systems). For western blotting, the following sample buffer and procedure should be used:Cells should be lysed by resuspending in 62.5mM Tris/HCI, pH 6.8; 6 M urea; 10% glycerol; 2% SDS; 0.00125% bromophenol blue; 5% B-mercaptoethanol, sonicated for 15 s and incubated at 65'C for 15 min. The sonication step and presence of urea is necessary to break up PARP/DNA interactions.

Positive Control: -HL-60 cells treated with etoposide

General Ref: 1. Shah,G.M., et al (1996) Biochem.  Biophys.Res. Commun. 229: 838-844.

                      2. Lamarre,D. et al., (1988). Biochem Biophys Acta 950: 147.


Storage: Store -20 DEG C or lower. Avoid frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. For prolonged storage add 0.2% BSA and 0.1% sodium azide to any dilution buffer.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Background:

PARP (Poly (ADP-ribose) polymerase [NAD+ ADP-ribosyltransferase;NAD+:poly adenosine-diphosphate-D-ribosyl)-acceptor ADP-D-ribosyltransferase, EC 2.3.2.30] is a highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+ dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in the apoptotic response of cells. The critical functional domains of the protein are the DNA binding domain and the automodification domains. PARP functions by adding poly (ADP-ribose), in an ATP and NAD+ dependent manner, to various proteins in response to DNA damage and has been shown to directly interact with DNA polymerase a. As a marker for apoptosis, PARP cleavage has been variably shown to occur early in the response as a result of the activity of CPP32 (caspase-3, CASP3). PARP cleavage correlates well with chromatin condensation and has been shown to be associated with the condensed chromatin in apoptotic cells, as a measure of apoptosis appearing as early as 3 hours post apoptosis inducing event, and precedes the ability to detect actual DNA fragmentation. Although shown in many systems to be a marker for apoptosis it is not clear that PARP cleavage is required for apoptosis to occur based on results from studies in PARP -/- mice. Degradation of PARP has also been shown to occur during the process of necrosis however an entirely different pattern of cleavage products is seen. In necrosis, major cleavage products of 89 and 50 kDa as well as minor cleavage products of 40 and 35 dDa have been reported. This latter pattern differs from that seen during apoptosis when only a major fragment of 89 kDa is observed.

For Research Use Only


Anti-PARP-1 Polyclonal Antibody

Catalog Number: RDI-ALS24279   $312.00/vial   $250.00/vial 5+

Quantity: 100ug in 0.1ml (1mg/ml) PBS containing 0.02% sodium azide.

                Bulk: cat#RDI-ALS24279X1 $1875.00/1mg

Description: Poly(ADP-ribose) polymerase 1, (PARP-1) and poly(ADP-ribose) polymerase 2 (PARP-2) catalyzes the formation of poly(ADP-ribose) polymers in a DNA-dependent manner. PARP-2 displays automodification properties similar to PARP-1. These act by poly(ADP-ribosylation), which is a post-translational modification of nuclear proteins in response to DNA damage that activates the base excision repair machinery.

Host Species: Rabbit

Specificity: Reacts with N-terminus of human PARP-1.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: Ame J.C. et al. J Biol Chem 1999 Jun 18;274(25):17860-8.

For research use only; not for use as a diagnostic.


Anti-PARP-2 Polyclonal Antibody

Catalog Number: RDI-ALS24280  $312.00/vial   $250.00/vial 5+

Quantity: 100ug in 0.1ml (1mg/ml) PBS containing 0.02% sodium azide.

                 Bulk: cat#RDI-ALS24280X1 $1875.00/1mg

Description: Poly(ADP-ribose) polymerase 1, (PARP-1) and poly(ADP-ribose) polymerase 2 (PARP-2) catalyze the formation of poly(ADP-ribose) polymers in a DNA-dependent manner. PARP-2 displays automodification properties similar to PARP-1. These act by poly(ADP-ribosylation), which is a post-translational modification of nuclear proteins in response to DNA damage that activates the base excision repair machinery.

Host Species: Rabbit

Specificity: Reacts with N-terminus of human PARP-2.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: Ame J.C. et al. J Biol Chem 1999 Jun 18;274(25):17860-8.

For research use only; not for use as a diagnostic.


All antibodes are for In Vitro Research Use Only-Not for Use in Diagnostics

-copyright by owner (s)


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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