rev:    November 9, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


-see below 2 abs to PACAP and 1 to PACAP R1
Polyclonal Antibody to PACAP 38 (Pituitary adenylate cyclase activating peptide)

cat#RDI-PRO16063    $688.00/vial

Category Rabbit polyclonal

Form/Purification Undiluted rabbit serum (lyoph.)

Immunogen Synthetic PACAP 38 (Pensinsula), conjugated to BSA

Description/Specificity Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. It occurs in two biologically active forms, PACAP-38 and PACAP-27. PACAP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts.

Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PRP, VIP, PHI, CRF, oxytocin and vasopressin do not.  Some cross-reactivity with PACAP-27.

Positive Control Stefanini-fixed frozen sections of rat duodenum or hypothalamus

Species tested so far    Human, rat

Application · Immunofluorescence microscopy

                   · Suitable for frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:400 - 1:800 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference Hannibal J et al. Gene expression of pituitary adenylate cyclase activating polypeptide (PACAP) in the rat hypothalamus. Regulatory Peptides 55, 133-148 (1995)

Precautions: For In vitro research Use Only. Not for use   in or on humans or animals or for diagnostic


Polyclonal Antibody to PACAP-related Peptide (Pituitary adenylate cyclase activating peptide-related peptide)

Cat#RDI-PRO16064    $688.00/vial

Category Rabbit polyclonal

Form/Purification Undiluted rabbit serum (lyoph.)

Immunogen Synthetic rat PRP (Pensinsula), conjugated to BSA

Description/Specificity      Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. PACAP-related peptide (PRP) is a 29-amino acid peptide, which is produced together with PACAP-27 and PACAP-38 when PreproPACAP is being processed. PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts.

Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not.

Positive Control Stefanini-fixed frozen sections of rat duodenum, fundus or antrum

Species tested so far     Rat; negative with human

Application · Immunofluorescence microscopy

                   · Suitable for frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:400 - 1:800 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference

Hannibal J et al. Gene expression of pituitary adenylate cyclase activating polypeptide (PACAP) in the rat hypothalamus. Regulatory Peptides 55, 133-148 (1995)

For Research Use Only


DATA SHEET: Rabbit anti-PACAP Receptor Type 1 (Human)

Catalog#: RDI-PACAPR1abR   $656.00/vial

Package Size: 50ug in 0.05ml (1mg/ml)PBS pH 7.7 with 0.01% sodium azide.

Species: rabbit IgG

Immunogen: Synthetic peptide (KLH) conjugated to the C terminus

Use: Immunohistochemistry: 3-16ug/ml (formalin fixed paraffin sections) positive control, human placental cells

Storage: Store at -20 DEG C. Avoid frequent freeze/thaw cycles

Background: The PACAP receptor type 1 expression has been documented in adipose, adrenal, bone, brain, colon, ganglion, heart, lung, ovary, pancreas, placenta, spleen, and uterus. ESTs have been isolated from brain and nerve libraries.

G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

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RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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