rev:  September 9, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Ornithine Decarboxylase (see below 2 new polyclonals and 1 monoclonal)
DATA SHEET: rabbit anti-ornithine Decarboxylase

-background: Ornithine Decarboxylase is the rat limiting enzyme in polyamine biosynthesis converting ornithine into putrescine. In normal tissue, ODC activity is low but increases in proliferating tissue.

Catalog#: RDI-PRO16061   $594.00/vial


Package Size: 50 microliters lyophilized rabbit serum.


Immunogen: human recombinant ODC


Application -immunofluorescence 1:300-1:500 overnight 4 DEg C

-All titers must be determined in assay

Reactivity: mouse, human, rat

Positive control: Steffanini-fixed frozen sections of renal cortex from testotosterone-treated mice

Storage: Upon receipt, store at 4 DEG C or below. -After reconstitution, aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

ref: Greenfield ARL et al, Cell Tissue Res. 243, 33-40 (1986)

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET:    Guinea Pig anti-ornithine Decarboxylase

-background: Ornithine Decarboxylase is the rat limiting enzyme in polyamine biosynthesis converting ornithine into putrescine. In normal tissue, ODC activity is low but increases in proliferating tissue.

Catalog#: RDI-PRO16062     $594.00/vial

Package Size: 50 microliters lyophilized guinea pig   serum.


Immunogen: human recombinant ODC


Application -immunofluorescence 1:500-1:800 -All titers must be determined in assay


Reactivity: mouse, human

Storage: Upon receipt, store at 4 DEG C or below.

-After reconstitution, aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-Ornithine Decarboxylase

cat# RDI-ORNITHDabm      $440.00/100ug

       RDI-ORNITHDabm-1   $688.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request)

Clone: MP16-2

Isotype: IgG1

Immunogen: A synthetic peptide (aa 345-360) from rat ODC coupled to BSA.

                    Sequence= K P D E K Y Y S S S I W G P T C

Fusion Partner: female BALB/c splenocytes and mouse myeloma SP2/0 cells

Uses: -Western blot: 1:100-1:200

          -Elisa

          -histochemistry (frozen sections) 1:100


Positive Control: epithelial cells in normal placenta or prostate carcinoma

Localization: Cytoplasmic

Mapping: recognizes a 53kDa protein identified as ornithine decarboxylase. Epitope localizes in aa355-360 (-IWGPTC) of ODC. This antibody recognizes the native ODC but not theinhibited enzyme.

Species Reactivity: human, mouse, rat. Suspected with all eukaryotes since binding epitope is -IWGPTC which is present in all known eukatyotic ODC sequences

Background: ODC mRna levels are reported to be elevated in lung carcinomas and in colon adenomas and carcinomas. ODC activity in colon carcinomas is greater than those in adenomas and normal mucosa.


ref: Schipper RG et al, J. Immunological Methods 193, 161(2):205-15

For Research Use Only




RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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