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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-Occludin

cat#RDI-OCCLUD1abm      $438.00/50ug

cat#RDI-OCCLUD1abm-1   $750.00/150ug

Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intercellular barriers between epithelial and endothelial cells. Among the barriers formed by the tight junctions is the critical blood-brain barrier. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1 and ZO-2. The human occludin protein is 522 amino acids with four transmembrane domains in the first half of the protein. Two glycine/tyrosine-rich extracellular loops are involved in cell-cell interactions. In the cytoplasm, occludin interacts with ZO-1 through its intracellular C-terminus. A possible function of ZO-1 may be to link the adherens components, like occludin, to the submembraneous actin cytoskeleton. Experimental breakdown of the tight junctions at the blood-brain barrier results in redistribution of vinculin and loss of ZO-1 and Occludin. Taken together, these data indicate that occludin and ZO-1 are critical elements in the formation of tight junctions and may also serve an important role in signaling and tumor suppression.

Isotype: mIgG1

Immunogen: peptide fragment from 396-507 (C terminus) from mouse Occludin

Packaging: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: Suitable for western blot on human, rat ,mouse and dog using approx 1 ug/ml ab on total cell lysate. Major band at Mw 65kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection.

-also for immunofluorescence

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: -Bolton, S.J., et al. 1998

                    -Fanning, A.S., et al. 1998 J. Biol. Chem. 273:29745- 29753

                    - Ando-Akatsuka, Y., et al 1996 J. Cell Biol. 133:43-47

Precautions: For In vitro research Use Only. Not for use in or on   humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Recommended western blot Positive Control:

Rat Brain Lysate Prepared by lysis and heat denaturation of rat brain in 1% SDS, 1.0mM sodium vanadate, 10mM Tris 7.4. Cell lysate is provided at a concentration of 1.0mg protein/ml in SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue and 5% glycerol). 5 -10µl/well is sufficient for Western blotting. Store at -20°C. Stable for at least one year.



-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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