rev:     October 1, 2001

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(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


cat# RDI-NUCLEOLabm   Price: $469.00/vial

Packaging: 100ug

Clone: 4E2

Isotype: mIgG1

Immunogen: Human Nucleolin purified from Raji cell extract

Specificity: This antibody detects human Nucleolin on: -western blot (approx 1ug/ml visualize band at 100 Kd) (pos control total cell lysate from HL60, ZR75-1, Jurkat or Raji)

-immunofluorescence: 1-10ug/ml

-immunohistochemistry: 1-10ug/ml (paraffin-embedded   sections should be processed under microwave treatment)

Use; allows in situ detection of nucleaolar protein in HEp- II cell and paraffin embedded tissue sections.

ref: -J. Biol Chem 271, 1993 (1996)

      -J. Biol Chem 267:2955 (1992)

      -Gene ,160:143 (1995)

Storage: Store at -20 DEG C. Avoid frequent warming cycles.

Background: Nucleolin is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. Nucleolin fluctuates in parallel to DNa synthesis. The intact 100kda nucleolin molecule is the major species in actively dividing cells, whereas the degraded forms are relatively abundant in nondividing cells. Stability of nucleolin molecule is cell proliferation-dependent. Nucleolin is found to be identical to the human DNA helicase IV. Nucleolin/DNA helicase IV can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Furthermore, nucleolin directly interacts with DNA Topoisomerase I.

For Research Use Only

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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