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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


NOS (Nitric Oxide Synthases)- ANTISERA
Nos1=  ncNOS (neuronal maps at 12q24.2)

NOS2= iNOS (inducible form, broad expression cells, maps 17cen-q12)

NOS3= eNOS (endothelial cells, maps 7q35-36)


Mouse anti-N NOS Monoclonal ab  (Nos 1)

cat# RDI-NNOSabm       $438.00/vial 50ug

       RDI-NNOSabm-1   $750.00/vial 150ug

Background: Nitric oxide synthatase (NOS) is a cell type specific enzyme whioch catalyzes the synthesis of nitric oxide (NO). NO is a short lived radical which transmits signals involved in vasorelaxation, neurotransmission and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular CA++ concentrations and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155kDa and 140kDa, respectiviely and the human form shows 52% amino acid identity to each other. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology, being most divergent in the calmodulin binding domain. Neuronal NOS is a cytosolic protein present mainly in neuronal tissues and has been purified and chacterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from vascular endothelium as well as from bovine aortic endothelial cells and has unique n-myristylation consensus sequence that may explain its membrane localization.


isotype: mIgG2a


package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)


antigen: a 22.3 kDa C-terminal region protein fragment corresponding to amino acids 1095-1289 of human n- NOS.


Use: -Suitable for western blot on human, rat and mouse using approx 0.1 to 0.5ug/ml ab on total cell of rat pituitary cells. Major band =MW 155kDa - Immunofluorescence (also on rabbit brain)


Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


Main REF: -Nathan, C. 1992 FASEB J. 6:3051

                  -Nathan, C et al 1994 J. Biol Chem 269:13725

                  -McKee, M et al, 1994:Proc Natl Acad Sci USA 91:12056

                  -Xie, Q.-W, et al. 1992:Science 256:225

-copyright by owner
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product. Also available:poly's against n-nos. i-nos and e-nos


Mouse anti-I NOS (Nos II) Monoclonal ab

cat# RDI-NOS2abm       $438.00/vial 50ug

       RDI-NOS2abm-1    $750.00/vial 150ug

Background: Nitric oxide synthatase (NOS) is a cell type specific enzyme which catalyzes the synthesis of nitric oxide (NO). NO is a short lived radical which transmits signals involved in vasorelaxation, neurotransmission and cytotoxicity. In macrophages and other cell types, Nos (iNOS,macNOS) activity increases following exposure to cytokines (IFN-G, TNF-A and IL-1) and microbial products (lipopolysaccharide LPS). iNOS is activated independently of Ca2+/Calmodulin and its level of expression is tightly controlled by several transcription factors including NFkB. Data indicates that TGF-B affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue,iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a C-terminal region protein fragment corresponding to amino acids 961-1144 of mouse iNos (in the NADPH Binding region)

Use: -Suitable for western blot on human, dog, rat and mouse using approx 0.1 to 0.05ug/ml ab on total cell lysate of mouse macrophages. Major band =MW 130kDa - -Immunofluorescence (on induced mouse macrophage) -immunoprecipitation:native/denatured

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


gen REF: -Nathan C et al, (1994) J. Biol Chem 269:13725

                -McKee, M eta l, 1994 Proc Natl acad Sci USA 91:12056

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-I NOS (Nos II) Monoclonal ab  mIgG2a isotype

cat# RDI-NOS2Babm          $438.00/vial 50ug

        RDI-NOS2Babm-1     $750.00/vial 150ug

Also available:FITC labeled cat#RDI-NOS2Babm-FT $469.00/50ug

isotype: mIgG2A

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a C-terminal region protein fragment corresponding to amino acids 961-1144 of mouse iNos (in the NADPH Binding region)

Use: -Suitable for western blot on human, rat and mouse using approx 0.1 ug/ml ab on total cell lysate of mouse macrophages. Major band =MW 130kDa - -Immunofluorescence (on induced mouse macrophage) -immunoprecipitation:native/denatured

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

REF: -Togashi, H et al 1997 Proc Natl acad Sci USA 94:2676- 2680.

         -Guan, Z et al 1997 J. biol Chem 272:083-8089

         -Meltzer et al 197 J. Histochem & Cytochem 45:599-610

         -Nathan C et al, (1994) J. Biol Chem 269:13725

       -McKee, M eta l, 1994 Proc Natl acad Sci USA 91:12056

Precautions: For In vitro research Use Only. Not for use in or on humans oranimals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-NOS3abm (ECNOS)

cat#RDI-NOS3abm        or cat#RDI-ECNOSabm        $438.00/50ug

       RDI-NOS3abm-1    or cat#RDI-ECNOSabm-1    $750.00/150ug

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)


antigen: a C-terminal region protein fragment corresponding to amino acids 1030-1209 of  human EcNos (in the NADPH Binding region)

Use: -Suitable for western blot on human, rat and mouse using approx 0.2ug/ml ab on total cell of human endothelial cells. Major band =MW 140kDa also suitable for IP ,Immunofluorescence of tissue cultured cells and immunohistochemistry.

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: Dinerman, J et al 1994: Proc Natl Acad Sci USA 91:4214-4218

                   Brenman, J., et al 1996: Cell 84:757-767

Togashi, H et al 1997 Proc Natl acad Sci USA 94:2676- 2680.

         -Guan, Z et al 1997 J. biol Chem 272:083-8089

         -Meltzer et al 197 J. Histochem & Cytochem 45:599-610

         -Nathan C et al, (1994) J. Biol Chem 269:13725

       -McKee, M eta l, 1994 Proc Natl acad Sci USA 91:12056

Precautions: For In vitro research Use Only. Not for use in or on humans oranimals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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