rev:    October 30, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


N-MYC ANTISERA

. See also abs to C-MYC and L-MYC.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN N-myc

PRODUCT CODE        RDI-CBL431        $375.00

CLONE                         IA8

ISOTYPE                     IgG2b

IMMUNIZING ANTIGEN Human N-myc sequence 334-343 + Cys.

SOURCE                      Tissue culture supernatant

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY        The antibody reacts with a major peptide-blockable band at 62-64 kDa on western blots.

APPLICATIONS     *Western blotting on detergent lysates from cells expressing N-myc.

                                 *Immunohistochemical studies of N-myc on frozen sections.

REFERENCES Moore, J. P. et al. Oncogene Res. 2, 65-80 (1987)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic


SHEEP POLYCLONAL ANTIBODY TO: HUMAN N-myc ONCOPROTEIN (Human/Mouse)

PRODUCT CODE              RDI-CBL437    $375.00

IMMUNIZING ANTIGEN Synthetic peptide sequence: Tyr-Gln-Gln-Leu-Leu-Lys-Lys-Ile-Glu-His-Ala-Arg-Thr-Cys, selected from exon 3 of the human N-myc oncoprotein. The sequence is totally conserved in mouse N-myc. The synthetic peptide was coupled to KLH using MBS.

SOURCE                                 Sheep serum

PURIFICATION METHOD   Affinity purified against immobilized synthetic peptide

SPECIFICITY The antibody detects a major peptide-blockable band at approximately 64 kDa in the following procedure. Detergent lysates were produced from cell lines expressing N-myc such as Kelly and IMR-32. The lysates were run on a 10% SDS-PAGE and electroblotted on to nitrocellulose. Affinity purified antibody pre-incubated with (and without) peptide was incubated on strips of the blotted lystate. The bound antibody was detected using a peroxidase labelled secondary antibody.

APPLICATIONS        *Immunoblotting

PRESENTATION The purified immunoglobulin is presented at a concentration of 100µg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


Mouse anti-N MYC   cat#RDI-NMYC2abm    $406.00/100ug vial in 0.2ml Tris (0.5mg/ml)

-mouse IgG2b clone N-MYC2 (N-MYC 1A8) (see EMBO 10:3703-3712)

-immunogen:synthetic peptide corresponding to amino acids 327-339 of human N-myc

(sequence= S P V E S E D A P P Q K C)

-human and mouse reactive

-binds to N-Myc, a helix loop-helix/leucine zipper protein. N-MYC migrates as one or more bands between 58-67kD in SDS-PAGE. Heterogeneity results from post-translational modification by phophorylation.

-suitable for western blotting

-immunoprecipitation:


The myc gene family contains at least seven closely related genes, c-myc, n-myc, l-myc, p-myc, r-myc,   s-myc, and b-myc. N-myc, c-myc, and l-myc proteins   each contain basic, helix-loop-helix and leucine zipper domains. All three form sequence-specific (CACGTG), binding heterodimers with the protein max which also contains all three motifs. The leucine zipper domain and the helix-loop-helix motifs of myc and max proteins contribute to heteerodimer formation and DNA binding of myc-max dimers results in a conformational change of the DNA. N-MYC also binds to asymmetric (CATGTC) sequences. N-Myc is a nuclear phosphoprotein that is thought to be involved in differentiation.
-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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