rev:  October 23, 2002

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Neurophysin


RABBIT ANTI-NEUROPHYSIN   (Human/Pig/Dog/Rat/Sheep/Cat)

cat# RDI-NEURPHYabr   $375.00

Presentation: 500 ul rabbit antiserum containing 0.1% sodium azide.

Immunogen: Purified porcine neurophysin-II.

SPECIFICITY: The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of neurophysin-containing structures in the hypothalamus and posterior pituitary glands using the immunoperoxidase and immunofluorescence procedures. This antibody does not distinguish between various forms of neurophysin.

STORAGE: Maintain at -20C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Use; Histochemistry:Formalin-fixed and paraffin embedded sections (6 microns thick) of hypothalmi and posterior pituitary tissue were collected and freeze-dried, from man, cat, rat, dog, ox and sheep

Dilution: approx 1:2500 (optimal titers should be determined for each use)

General Protocol:

IMMUNOPEROXIDASE STAINING:

1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).

2. The antiserum diluted 1:2,500 (in PBS) is allowed to react with the tissue at 37C for 30 minutes.

3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37C. After each of these steps, the tissue is washed with PBS as before.

4. The final stage of the reaction is treatment of the section with a mixture of 3,3’ -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.

IMMUNOFLUORESCENCE STAINING:

1. Same as Step 1 above.

2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37C.

3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.

REFERENCES:

1. Nairn, R. C. Fluorescent protein tracing. Livingstone, Edinburgh (1964).

2. Sternberger, L. A. Immunocytochemistry. Wiley, New York (1979).

3. Rhodes, C. H., et al. Neuroendocrinology, 33:18-23 (1981).

4. Sofroniew, M. V., et al. Neuroscience, 6:619-643 (1981).

5. Neuroscience, Vol 48, 2:383-395 (1992).

For research use only:


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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