rev: February 25, 2004
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(anti-Human and others
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Anti-NESTIN (see 2 antibodies below)
Background: nestin is an intermediate filament protein that is abundantly expressed in neuroepithelial stem cells early in embryogenesis, but is absent from nearly all mature central nervous (CNS) cells. Nestin was originally defined as the antigen recognized by the monoclonal Rat401.
Catalog#: RDI-RTNESTINabm $438.00/vial 0.1mg
Package Size: 100ug in 0.2ml (0.5mg/ml) 10mM sodium phosphate, 150mM NaCl with 0.09% sodium azide. (sodium azide is toxic,dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)
Prep: purified from TCS by Protein G
Species: mouse IgG1
Antigen: paraformaldehyde fixed spinal cord from day 15 rat embroys.
Activity: -recognizes rat nestin (does not cross react with human nestin). (1)
-western blot (2) doublet of approx 200kD, RAT- 401 positive cells are found throughout the
developing neural tube, but not in the adult CNS. More than 90% of dissociated E11 neural tuvbe cells are rat-401 positive.
-flow cytometry (3)
-histochemistry on frozen sections (1 & 2)
-indirect immunofluorescence of tissue cultured cells (2)
1) J. Neurosci 5:3310-3328
2) J. Neurosci 9:4303-4317 & New Biologist 3:364- 371
3) J. Neurosci 8:1144-1151
4) Am J Pathol 143:258-268
Precautions: For In vitro research Use Only.
cat# RDI-NESTINabm $440.00/vial 50ug
RDI-NESTINabm-1 $750.00/vial 150ug
Background: The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratin proteins expressed in epithelia cells, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin proteins expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an alpha helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.
Antigen: derived from rat nestin aa 402-604
package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.
Use: Suitable for western blot on human, rat ,and mouse using approx 0.5ug/ml ab on total cell lysate. Major band at Mw 260kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection. Not suitable for immunofluorescence.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product. -copyright by owner
Recommended positive western blotting Control:for Nestin monoclonal
Rat embryonic (E17) cerebrum lysate: Do not boil or dilute.
cat#RDI-RTEMCEREL-CPX $250.00/500ug vial
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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