rev:  February 10, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


-see also acetylcholine and NMDA receptor antibodies


Mouse anti-Nicotinic Acetylcholine Receptor Alpha 4 (monoclonal)

cat# RDI-NARA4abm-A4   $500.00

Presentation: Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute with 1 ml sterile distilled water . Let set at least 30 minutes, mix well.

Clone: 8A4

Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).

Isotype: mIgG2a

Immunogen: Prokaryotic recombinant protein corresponding to the major cytoplasmic domain of the nicotinic acetylcholine receptor alpha 4 molecule.

Specificity: Human nicotinic acetylcholine receptor alpha 4

Use: Effective on paraffin wax embedded tissue using citrate buffer unmasking solution combined with high temperature antigen unmasking techniques.

Recommendations on use: Immunohistochemistry: Typical working dilution 1:100-1:200 60 minutes primary antibody incubation at 25 DEG C Standard ABC technique.

-not tested on frozen sections

-not recommended for Western blotting:

Positive controls: Hippocampus

Staining pattern: cytoplasmic

Storage: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20'C (frost- free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

For Research Use Only

Application:

Acetycholine is a neurotransmitter which can control the neuritogenesis of hippocampal cells. The time at which receptors for acetylcholine are expressed may influence synaptogenesis and neuronal activity in developing hippocampus. In fully developed, adult hippocampus of mice, the expression of nicotinic acetylcholine-gated ion channel receptor alpha4 subunit is low. In embyronic mouse, expression is initially low, then rises to a maximum in only the second week of life and finally decreases to a minimum in adulthood. This transient expression profile of the alpha4 subunit suggests that nicotinic acetylcholine receptors may participate in the early postnatal maturation of hippocampal circuitry. Investigators of rat brain using immunohistochemistry for the alpha4 subunit indicates the staining of cell bodies and apical dendrite of pyramidal cells. This antibody may be useful for further studies of the functional role of nicotinic acetylcholine receptor alpha4 in the human brain during embryonic development , mayuration and lesion repair.

References:

Nakayama H, Shioda S, Okuda H et al. Immunocytochemical localization of nicotinic acetylcholine receptor in rat cerebral cortex. Molecular Brain Research 32:321-328 (1995)



Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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