rev:      May 7, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MMP-8  Matrix metalloproteinases-8
Matrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the eleven proteins reported to date, ten are normally found as soluble molecules. MMP-8, also known as polymorphonuclear leukocyte collagenase, is a collagenolytic enzyme first described in 1986. MMP-8 is closely related to the fibroblast collagenases (47% homology) but is encoded by a different gene.The enzyme exists as an inactive pro form with a molecular weight of approximately 85 kDa of which 35% is contributed by glycosylation. MMP-8 becomes activated by removal of the amino terminal pro-peptide which can be effected by trypsin but not by cell bound plasmin in a two step process. MMP-8 also will undergo autoproteolysis a process which can also be mediated by mercurial compounds. Both processes are inhibited by TIMP-2, which bind to the catalytic domain of the enzyme with a 1:1 stoichiometry and is partially resistant to dissociation under SDS-PAGE conditions. The active enzyme has been found to be unstable at 37'C, breaking down into two fragments of 40 kDa and about 27 kDa. Of the two fragments the 40 kDa one has been identified as the catalytic domain and is biologically active while the other is the COOH  terminal domain and is inactive.
DATA SHEET:  Mouse anti-human MMP-8 monoclonal (WB)

Catalog#: RDI-MMP8abm-D2   Price: $438.00/vial

Package Size: 100ug (lyophilized) with 100ug BSA. Reconstitute with 1ml sterile PBS pH 7.4 or sterile 20mM Tris-saline (20mM Tris containing 0.15 NaCl). Allow to set for 2 hours at 4 DEG C prior to use or further dilution. Mix well before use. If suitable for use, addition of 0.1% sodium azide is recommended.

Ig Isotype: mouse IgG1

clone: 115-13D2

Antigen: purified human MMP8

Reactivity: Binds to human MMP-8. not epitope mapped

Suitable for: western blotting (5ug/ml)using chemiluminescence detection

-MMP8 exists as both latent (85 kDa) and active (64kDa) forms. Recognizes both forms

-Paraffin sections (fix with PLP (periodate- lysine-4% paraformaldehyde) and culture tissue in monensin prior to fixation see Matsuki,H et al (1996) Clin Chima Acta 244:129-143

Positive Control: PMNL cells

General Ref: Eur J. Biochem 189:295-300

                     Biochem J. 291:847-854

Storage: Store 4 DEG C or lower. Avoid frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. For prolonged storage add 0.02% thimerosal or 0.1% azide.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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