rev:   July 27, 2001

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MMP-7  (matrix metalloproteinase-7)
The matrix metalloproteinases (MMPs) are a family of enzymes responsible for the degradation of extracellular matrix components. Of the eleven proteins reported to date, ten are normally found as soluble molecules. MMP-7, also known as matrilysin, is responsible for hydrolyzing proteoglycans and extracellular matrix glycoproteins and was first described in 1980 but not purified to homogeneity until 1988. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. The enzyme exists as an inactive pro from with a molecular weight of approximately 28 kDa (range: 28-30 kDa) which becomes activated by proteolytic cleavage to an active 19 kDa form. Pro-MMP7 may  be activated by trypsin, mercurial compounds or by MMP-3 and once activated can itself activate pro-MMP1 and pro-MMP-9 but not pro-MMP-1. MMP-7 is distinguished from the other MMP family members as being the smallest member containing only the common catalytic domain and the Zn2+ binding region but missing the hemopexin-like domain common to the other MMP's. Matrilysin is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of glimoas.
DATA SHEET:   Mouse anti-human MMP-7 (pro) monoclonal (WB/Paraarin Section


Catalog#: RDI-MMP7abm-B2   Price: $438.00/vial

Package Size: 100ug (lyophilized) with 100ug BSA. Reconstitute with 1ml sterile PBS pH 7.4 or sterile 20mM Tris-saline (20mM Tris containing 0.15 NaCl). Let set at least 2 hours at 4 DEG C. Mix well before using. If suitable for use, addition of 0.1% sodium azide is recommended.

Ig Isotype: mouse IgG1

clone: 141-7B2

Antigen: purified human MMP7 from rectal carcinoma (CaR-1) cells


Reactivity: Binds to human pro-MMP-7 (28kDa ) but NOT active/latent (19kda)  MMP-7. not epitope mapped

                    -no cross with human MMP-1,-2,,-3,-8,-9 pr MMP-13.

Suitable for: -western blotting (5- 10ug/ml)usingemiluminescence detection

                   -paraffin sections

Positive Control: TPA treated (100ng/ml) SW620 cells or recombinant PRO-MMP-7 cat#RDI-MMP7PRO-AG $438.00/100ug

APPLICATION REFERENCES: Paraffin Sections and Western Blotting

                Ohta, S. et al. (1998) Lab. Invest. 78: 79- 87.

                Yamashita, K. et al. (1998) Int. J. Cancer 79: 187-194.


General Ref: Meth Enzymol. 248:485-495

                      J. Biol Chem 270:6691-6697

                      J. Biol Chem 269:2032-2040

Storage: Store 4 DEG C or lower. Avoid frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. For prolonged storage add 0.02% thimerosal or 0.1% azide.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-not for sale in Japan


DATA SHEET:Mouse anti-human MMP-7 monoclonal (WB) (Active Form)

Catalog#: RDI-MMP7abm-F12     Price: $438.00/vial

Package Size: 100ug (lyophilized) with 100ug BSA. Reconstitute with 1ml sterile PBS pH 7.4 or sterile 20mM Tris-saline (20mM Tris containing 0.15 NaCl). Let set at least 2 hours at 4 DEG C. Mix well before using. If suitable for use, addition of 0.1% sodium azide is recommended.

Ig Isotype: mouse IgG1

clone: 176-5F12

Antigen: peptide (YSLP) corresponindg to amino acids 78-82 of human MMP7.



Reactivity: Binds to human active MMP-7 (19kda) but NOT PRO MMP-7 (28kDa)

no cross with himan MMP-1, -2, -3, -8, -9 or -13.

Suitable for: western blotting (5- 10ug/ml)using chemiluminescence detection

Positive Control: SW620 cells (treated with 100ng/ml TPA)  or recombinant active MMP-7 cat#RDI-MMP7-AG $438.00/100ug

General Ref: Meth Enzymol. 248:485-495

                     J. Biol Chem 270:6691-6697

                     J. Biol Chem 269:2032-2040

Storage: Store 4 DEG C or lower. Avoid   frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. For prolonged storage add 0.02% thimerosal or 0.1% azide.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-not for sale in Japan


Rabbit Anti-MMP-7   [Matrilysin, PUMP, uterine metalloproteinase)

CAT#: RDI-MMP7Nabr   $469.00/VIAL

Quantity: 100 ug of Peptide-affinity purified immunoglobulin. In 0.1ml 50% glycerol/buffer

Specificity: binds to human MMP-7 but does not cross react with MMP family members (also sees other non-human forms).

Immunogen: Synthetic peptide based on the amino terminal domain of human MMP-7. The sequence is based on the human sequence, but the antibody also recognizes non-human forms of MMP-7.

Applications: -western blot: approx 1:5000 with chemiluminescence detection. When used against the reduced protein, recognizes a band at 28kda for the zymogen and at 18kDa for the active enzyme. Recognizes both active and latent MMP-7. MMP-7 levels in quiescent cells and tissues is minimal and stimulation or protein concentration is often needed to visualize the bands. In additon, cells types differ greatly in the quantity of MMP-7 produced.

-immunoprecipiation

-Elisa

-histochemsitry (not tested) recommend ABC amplified detection, acetone fixed frozen sectiosn or fixed cells.

Optimal working dilutions must be determined by the end user.

Storage: Store -20 DEG C in working aliquots up to 1 year. Avoid frequent warming cycles.

For Research use only: not for use as a diagnostic.


also available:recombinant human MMP-7    cat#RDI-MMP7-AG   $438.00/100ug

recombinant human MMP-7 (proenzyme)   cat#RDI-MMP7PRO-AG    $438.00/100ug


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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