rev: April 15, 1998

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MMP-15/MT2-MMP Antibodies

see also anti-MMP-1, MMP-2, MMP-3, MMP-7 , MMP-8, MMP-9 and MMP-13, MT1-MMP (MMP14)


Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several of the MMP proteins have been shown to be integral membrane proteins and have been named MT-MMP's for membrane bound MMP . The MT-MMP family is now known to contain at least three members, MT1-MMP, MT2-MMP and MT3-MMP also known as MMP14, MMP15 and MMP16 respectively. While each of these proteins contain a C-terminal transmembrane domain allowing localization to the cell surface they are independent in expression. These proteins are also unique from the other members of the MMP family in that they contain an 8 amino acid insert in the catalytic domain.

The MT2-MMP protein is encoded by a 3.6 kb mRNA species giving rise to a protein with a molecular weight of 68 kDa by SDS-PAGE. While it appears that MT1-MMP is responsible for cleaving progelatinase A (MMP-2, 72 kDa Type IV collagenase) to its active form , MT2-MMP plays a minor role in activation of proenzyme  MMP-2 in breast carcinomas. However, coexpression of MT1-MMP and MT2-MMP in advanced stages of breast carcinomas suggests possible involvement of MT2-MMP in the metastasis.


DATA SHEET:Mouse anti-human MT2-MMP(MMP-15) monoclonal   (WB/paraffin sections)

Catalog#: RDI-MT2MMPabm    Price: $438.00/vial

Package Size: 100ug (lyophilized)with 100ug BSA.

Dilution: Tighten cap, tap vial to settle contents. Reconstitute with 1ml sterile PBS pH 7.4 or sterile 20mM Tris-saline (20mM Tris containing 0.15M NaCl) pH 7.4. allow to set 2-3 hours at 4 DEG C, mix well. Addition of 0.1% sodium azide recommended or other preservative if suitable for your system (do not add azide if to be used in neutralization type reactions)

Ig Isotype: mouse IgG1

clone: Clone 162-22G

Antigen: oligopeptide of DTDNFQLPEDDLRG (residues 281-294) on mouse MT2-MMP.

Reactivity: Binds to human MT-MMP-2 (70kDa)

Suitable for: western blotting (approx 10ug/ml)using enhanced cheiluminescence detection, positive control HT-1080 cells.

                     -immunoprecipitation

                     -histochemistry (paraffin sections) 20ug/ml with breast carcinoma as positve control)

Ref: -Ueno, H et al (1997) Cancer Research 57:2055-2060

       -Sato, H et al (1994) Nature 370:61-65

       -Yu, M et al (1995) Cancer Res 55:3272-3277

Storage: Store 4 DEG C or lower. Avoid   frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. Recommend adding 0.1% azide if suitable for your assay system. Dilution buffers should contain 0.1-1% BSA.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

see also anti-MMP-1, MMP-2, MMP-3, MMP-7 ,MMP-8, MMP-9 and MMP-13

-For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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