rev:    October 1, 2001

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MMP-10 (Matrix Metalloproteinase 10/Stromelysin-2)

The matrix metalloproteinases (MMPs) are a family of enzymes responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several other MMP proteins have been shown to be integral membrane proteins and have been named MT-MMP's for membrane bound MMP.

MM-10, also known as stromelysin-2 is the second member of the stromelysin subfamily and a potent MMP with similar substrate specificities as stromelysin 1. Its gene maps to chromosome 11 and its mRNA is expressed in a variety of cells and tissues including fibroblasts, keratinocytes as well as by a number of tumors such as oral squamous carcinoma, head and neck squamous cell carcinomas. The enzyme exists as an inactive pro form with a molecular weight of approx 56kDa which becomes activated by proteolytic cleavage to an active 47kDa form. Once activated MMP-10 can itself activate pro-MMP-1, pro-MMP-7, pro-MMP-8 and pro-MMP-9 but not pro-MMP-2 or pro-MMP-3.


DATA SHEET: Mouse anti-human MMP-10 monoclonal (WB)

Catalog#: RDI-MMP10abm-9b  Price: $438.00/vial

Package Size: 100ug (lyophilized) with 100ug BSA. Reconstitute with 1ml sterile PBS pH 7.4 or sterile 20mM Tris-saline (20mM Tris containing 0.15 NaCl). Let set at least 2 hours at 4 DEG C. Mix well before using. If suitable for use, addition of 0.1% sodium azide is recommended.

Ig Isotype: mouse IgG1

clone: 142-9B11

Antigen: synthetic peptide corresponding to amino acids 250-268 of human MMP-10

- A S T E E P L V P T K S V P S G S E M

Reactivity: Binds to human MMP-10. It also reacts slightly with MMP-3. The antibody reacts both the pro form (56kDa) and the active form (47kDa) of human MMP-10. not epitope mapped

Suitable for: western blotting (10ug/ml)using chemiluminescence detection

-not tested for other applications

Positive Control: culture media of OSC-20 cells

General Ref: -Biochem 28:5195-5203

                     -Eur J. Biochem 253:67-75

Storage: Store 4 DEG C or lower. Avoid frequent freeze thaw cycles. Antibody is stable for at least 6 months when stored frozen undiluted and up to 1 month at 4 DEG C when neat or diluted as per above recommendation. For prolonged storage add 0.02% thimerosal or 0.1% azide.



see also anti-MMP-1, MMP-2, MMP-3, MMP-7 ,MMP-8, MMP-9 and MMP-13



Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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