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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Specialty anti-mouse Macrophage related Antibodies  (see also anti-rat macrophage related antibodies)

-see also mouse CD reactive antibodies


Comparison chart of different mature macrophage markers

Moma-2 F4/80 BM8 ER-BMDM1
monocytes + + + -
kupffer cells + + + -
langerhans cells +- + +
tingible body macrophages + - -
interdigitating cells +- - - +
dendritic cells +- - - +
microglial cells - + - -
marginal zone macrophages - - - ?
marginal metallophillic cells - - - -
pneumocytes type II +
alveolar lavage cells 41% 66% 26%
resident perinotoneal cells 47% 51% 34%
thioglycollate elicited peritoneal cells

time after injection 8

                               4hours

17%

73%

28%

81%

15%

79%

bone marrow cells 14% 27% 37% 5%
bone marrow cells after 7 days in

culture supplemented with M-CSF

30% 90% 96% 91%


DATA SHEET:Rat anti-mouse Macrophages, monoclonal clone CI:A3-1

Catalog#: RDI-T-2008X   $625.00

CI:A3-1

Product Information

Murine F4/80 antigen (Monocytes / Macrophages)

CI:A3-1 recognizes the F4/80 antigen, a 120-160kD glycoprotein containing 7 EGF-like domains at the N-terminus, an RGD (Arg-Gly-Asp) integrin binding motif, and significant homology to the transmembrane 7 (Tm7) hormone receptor family at the C-terminus. The molecular structure suggests multiple ligands. Possibly, F4/80 is involved in macrophage adhesion within certain tissues, combined with receptor signaling following peptide-ligand interaction. The antigen is expressed by most macrophages and macrophage precursors beyond M-CFC, and increases upon maturation. Activated macrophages, and eosinophils express low levels. Freshly isolated spleen dendritic cells are uniformly positive for F4/80, but the expression decreases upon culture. This antibody is very useful for characterising tissue fixed macrophages, particularly in combination with BM 8 (product RDI-T2006X) and MOMA-2 (product RDI-T2007X).

Applications: Immunohistology on frozen and paraffin embedded sections, FACS, Immunoprecipitation, Western Blot.

Lot number:

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2b

Purity: Purified from culture supernatant

Physical state: Liquid

Quantity: 500ug in 500ul PBS, pH 7.2

Preservative: 0.1% Sodium azide

Storage and Stability: 1 month at 4°C, for longer periods at -20°C

Approximate working dilution: 2mg/ml on cryosections, 5mg/ml on paraffin sections, 10ml stock solution to stain 106 cells. Optimal concentration should be tested by serial dilutions.

This product requires trypsinisation of formalin fixed tissues.

Caution: this product contains sodium azide, a poisonous and hazardous substance For laboratory use and research purposes only

SELECTED LITERATURE

Hume, D. et al.: The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen. J. Exp. Med.: 158, 1522 - 1536 (1983).

Felix, R.et al.: Impairment of Macrophage Colony-stimulating Factor Production and lack of resident bone Marrow Macrophages in the osteoporotic op/op Mouse. J. Bone & Mineral Res.: 5/7, 781 - 789, (1990).

Sunderkotter, C. et al.: Cellular events associated with Inflammatory Angiogenesis int the Mouse Cornea. Am. J. Path. 138, 931 - 939, (1991).

Kraal, G. et al.: Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2; An Immunohistochemical Study. Scand. J. Immunol.: 26, 653 - 661 (1987).

BIOLOGICAL CHARACTERISTICS

Specificity

Mouse: monocytes, macrophages

Other species: unknown

BIOCHEMISTRY

CI:A3-1 detects a 160kD membrane protein of the F4/80 antigen.

ANTIGEN DISTRIBUTION

Isolated cells:

F 4/80 is recognised on monocytes of the peripheral blood and the bone marrow.

Tissue sections:

The determination of F 4/80 is already widely used for the detection of tissue macrophages. F4/80 positive macrophages, however, consist of different subpopulations from those detected with BM 8 or MOMA-2.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of   heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:Rat anti-mouse PAN monocyte/Macrophages, monoclonal clone MOMA-2

RDI-T2007X     $470.00 Monoclonal Antibody

Product Information

Anti Murine Monocytes / Macrophages

MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with BM8 and F 4/80 it allows a more precise characterisation of tissue fixed macrophages of various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface.

Application: Immunohistology on fresh frozen tissues, FACS (permeabilisation preferable)

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2b

Quantity 200mg

Volume 200ml

Purity: Purified from tissue culture supernatant

Physical state: liquid

Buffer: Phosphate buffered saline pH 7.2

Preservative: 0.1% Sodium azide

Stabilizer: none

Storage and Stability: 1 month at 4°C, for longer periods at -20°C

Approximate working dilution: 0.5mg/ml (1:1600)

Fixation: Dry acetone, 4°C.

Note: Only fresh frozen tissues should be used for MOMA-2.

Caution: this product contains sodium azide, a poisonous and hazardous substance. For laboratory use and research purposes only.

SELECTED LITERATURE

BREEL,M., MEBIUS,R.E., KRAAL,G.: Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur. J. Immunol.: 17, 1555 - 1559 (1987).

KRAAL,G., REP,M., JANSE,M.: Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2; An Immunohistochemical Study. Scand. J. Immunol.: 26, 653 - 661 (1987).

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Mouse: monocytes, macrophages

Other species: unknown

BIOCHEMISTRY

MOMA-2 detects a (glyco-)protein of 140kD m.w. which is located within the cytoplasm and on the cell surface. Attempts to isolate the antigenic determinant by immunoprecipitation and immunoblotting have so far been unsuccessful.

ANTIGEN DISTRIBUTION

Isolated cells:

In the cytospin preparation of thiogycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells

Tissue sections:

MOMA-2 detects typical tissue macrophages as do F4/80 or BM8. However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where F 4/80 and BM8 are negative.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:Rat anti-mouse Pan Tissue Fixed Macrophages, monoclonal clone BM8

cat#RDI-T2006X      $595.00

Product Information

Anti Murine F4/80 - Pan Macrophages

BM8 recognizes the F4/80 antigen on major subpopulations of resident tissue macrophages. The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model.

Applications: Histology on frozen and paraffin embedded sections, FACS

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Purity Affinity Purified

Physical State: Freeze-dried

Quantity: 100µg

Buffer: Phosphate buffered saline, pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.1% sodium azide

Reconstitute in: 0.5 ml distilled water (=stock solution)

Storage: unreconstituted: 4°C for 1 year

after reconstitution: aliquot and freeze at -20°C or lower

do not freeze working dilutions!

Stability of stock solution: approx. 1 year at -70°C

Approximate working dilution: optimal concentration should be tested by serial dilution

Histology 0.5mg/ml (1:400) freshly prepared on frozen sections

Fixation: Acetone, Glutaraldehyde, Formaldehyde / Paraffin

This product requires proteinase treatment of paraffin sections.

Caution: this product contains sodium azide, a poisonous and hazardous substance. For laboratory use and research purposes only.

SELECTED REFERENCES

Schaller, E. et al.: Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol. Cell Biol. 22: 8035-43 (2002).

Malorny, U. et al.: A monoclonal antibody angainst an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 243, 421-428 (1986).

Kraal, G. et al.: Macrophages in T and B Cell compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2......... Scand. J. Immunol. 26, 653-661 (1987).

Leenen, P.J.M. et al.: Markers of mouse macrophage development detected by monoclonal antibodies. J. Immunol. Methods, 174: 5-19 (1994).

Smit, M.J. et al.: Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells............ Hepatology 12: 1192-1199 (1990)

Rosmalen, J.G.M. et al.: Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates....... Lab. Invest. 80: 23-30 (2000)

BIOLOGICAL CHARACTERISTICS SPECIFICITY

Mouse: major subpopulation of resident tissue macrophages

Other species: human heart macrophages

BIOCHEMISTRY

The antigen is a 125kD extracellular membrane protein sensitive to 2-mercaptoethanol.

ANTIGEN DISTRIBUTION

Isolated Cells:

The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.

Tissue Sections:

The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-also available:


Anti Murine Pan Macrophages  Clone BM8  Biotin conjugated cat#RDI-T2017X   $656.00/150ug

BM8 is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model.

Applications: Histology on frozen and paraffin embedded sections, FACS

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Purity Affinity Purified

Physical State: Lyophilized

Quantity: 150µg

Buffer: Phosphate buffered saline, pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Reconstitute in: 0.5 ml distilled water (=stock solution)

Storage: unreconstituted: 4°C for 1 year

after reconstitution: aliquot and freeze at -20°C or lower

do not freeze working dilutions

Stability of stock solution: 1 year at -70°C

Approximate working dilution: optimal concentration should be tested by serial dilution

Histology 0.5mg/ml (1:800)

Fixation: Acetone, Glutaraldehyde, Formaldehyde / Paraffin

Caution: this product contains Thimerosal, a poisonous and hazardous substance. For laboratory use and research purposes only.

SELECTED REFERENCES

Malorny, U. et al.: A monoclonal antibody angainst an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 243, 421-428 (1986).

Kraal, G. et al.: Macrophages in T and B Cell compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2, an Immunohistochemical Study. Scand. J. Immunol. 26, 653-661 (1987).

Malorny, U. et al.: Immunohistochemical demonstration of migration inhibitory factor (MIF) in experimental allergic contact dermatitis. Clin. Exp. Immunol. 71, 164-170 (1988).

Leenen, P.J.M. et al.: Markers of mouse macrophage development detected by monoclonal antibodies. J. Immunol. Methods, 174: 5-19 (1994).

Smit, M.J. et al.: Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells involved in receptor-mediated endocytosis of lactate dehydrogenase and other enzymes. Hepatology 12: 1192-1199 (1990)

Rosmalen, J.G.M. et al.: Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates: Correlation with the Development of Diabetes. Lab. Invest. 80: 23-30 (2000)

BIOLOGICAL CHARACTERISTICS SPECIFICITY

Mouse: major subpopulation of resident tissue macrophages

Other species: human heart macrophages

BIOCHEMISTRY

The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2-mercaptoethanol.

ANTIGEN DISTRIBUTION

Isolated Cells:

The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.

Tissue Sections:

The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.


DATA SHEET:Rat anti-mouse Mid stage Macrophage Precursor Cell , monoclonal

CLONE ER-MP54   Catalog#: RDI-T2003X   $594.00

ER-MP 54

Product Information

Murine Early Stage Myeloid Precursor Cells

ER-MP54 is a general marker for detecting myeloid precursor cells in the bone marrow. The antigen is detected on a range of macrophage precursor cells up to the monoblast level.

Application: Histology, FACS

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG1

Purity / Physical State Purified / Freeze-dried

Quantity 100ug

Buffer: PBS pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Reconstitute in: 0.5ml distilled water (=isotonic stock solution)

Storage: unreconstituted: 4°C for 1 year

after reconstitution: aliquot and freeze at -20°C or lower

do not freeze working dilutions!

Stability of stock solution: approx. 1 year at -70°C

Approximate working dilution: 1mg/ml (1:300) freshly prepared

Fixation: Acetone, 0.05% Glutaraldehyde

For laboratory use and research purposes only. Caution: this product contains Thimerosal a hazardous and dangerous substance!

SELECTED REFERENCES

Leenen, P.J.M., Melis, M., Slieker, W.A.T, Van Ewijk, W.: Murine Macrophage Precursor Characterization II. Monoclonal Antibodies against Macrophage Precursor Antigens. Eur. J. Immunol. 20: 27-34 (1990).

Leenen, P.J.M., Melis, M., Slieker, W.A.T, Van Ewijk, W.: Murine Macrophage Precursor Characterization I. Production, phenotype and differentiation of macrophages precursor hybrids J. Immunol. 20: 15-25 (1990).

Leenen, P.M.J., M.F.T.R. de Bruijn, J.S.A. Voerman, P.A. Campbell, W. van Ewijk: Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Meth 174: 5-19 (1994)

BIOLOGICAL CHARACTERISTICS

Specificity:

Mouse: Myeloid precursors up to monoblats, cytoplasmatic antigen

Other: unknown

Immunogen:

Mouse macrophage cell lines, where the antigen is detected on the cell surface.

Antigen:

Multiple antigens were precipitated by ER-MP54 under reducing and non-reducing conditions: Major bands could be detected at 90, 85-80 and 75-70 kDa, at lower amounts at 45kDa and less frequently at 27 and 12 kDa.

Antigen Distribution:

Isolated cells:

The antigen is present on bone marrow myeloid cells up to monoblasts where the antigen is localized in the cytoplasm. Under specific experimental in vitro conditions, treatment by glucocorticoids, the antigen can also be detected on the cell surface.

Tissue sections:

The antigen can be detected in bone marrow plugs and some clusters of red pulp in spleen.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of   heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:  Rat anti-mouse Hematopoisis Associated Macrophages, monoclonal

Catalog#: RDI-T2012X      Price: $594.00/vial

Lot# see sheet with shipmen

Package Size: 500ug


Supplied: purified

Clone: ER HR-3


Ig Isotype: Rat IgG2c

Reactivity: Clone ER HR3 is a useful marker for the identification and localization of a very distinct macrophage sub-population found in various organs. This marker is especially suitable for ontogenetic studies because ER-HR3 positve macrophages are cosely related to hemopoietic islands, especially at erythropoietic sites.

Use: -Immunocytochemistry on formalin fixed paraffin or acetone fixed or frozen sections.

        -flow cytometry


ref: Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis, Vol H8, p.237, I.N. Rich ed., Springer Verlag (1987)

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of   heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federalrules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:Rat anti-mouse Splenic Marginal Zone Macrophages, monoclonal


Catalog#: RDI-T2010X   Price:  $594.00/vial


Lot# see sheet with shipmen

Package Size: 150ug

Supplied: purified

Clone: ER TR 9


Ig Isotype: Rat IgM

Reactivity: Clone ER TR 9 is useful for the identification of macrophage subpopulations present in the splenic marginal zone, lymph medulla and other organs. In combination with clone MOMA-1, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER TR 9 is also useful when studying phagocytosis of neutral polysaccharides as the antibody selectively inhibits uptake of these glycans by macrophages.

Use: -Immunocytochemistry on acetone fixed tissues

         -flow cytometry


ref: J. Histochem. Cytochem. 33, 40 (1985)

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:  Rat anti-mouse Pan Tissue Fixed Macrophages, monoclonal MOMA-1

Catalog#: RDI-T2011X     Price:  $595.00/vial

-also available Biotin conjugated cat#RDI-T2021X  $656.00/200ug

MOMA 1

Product Information

Murine metallophilic macrophage marker

MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by ER-TR 9. In addition , MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF).

Application: Immune histology

Lot number:

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Purity / Physical State Purified / lyophilized

Quantity 200ug

Reconstitute in: 0.5 ml distilled water (=isotonic stock solution)

Buffer: Phosphate buffered saline pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Storage: unreconstituted: 4°C for 1 year  after reconstitution: aliquot and freeze at -20°C or lower  do not freeze working dilutions.

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 4mg/ml (1:100) freshly prepared

Fixation: Acetone

For laboratory use and research purposes only. Caution: this product contains Thimerosal, a hazardous and dangerous substance!

Literature:

Kraal, G., Janse, M.: Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. Immunology 58, 665-669 (1986).

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Mouse: Subpopulation of mature resident tissue macrophages

Other: unknown

Immunogen: mouse macrophage cell lines

ANTIGEN

The antigen is a cytoplasmic and cell surface component.

ANTIGEN DISTRIBUTION

Isolated cells:

No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.

Tissue sections:

Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the inerfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET: Rat anti-mouse Macrophage-aminopeptidase),   monoclonal (CD13?)

Catalog#: RDI-MS-ERBMBM1

Price: $562.00/vial  


Package Size: 100 tests

Clone: ER-BMDM1

Ig Isotype: Rat IgG2a

Reactivity: Clone ER-BMDM1 is a useful for in situ and in vitro identification of aminopeptidase N positive macrophages, interdigitating cells and dendritic cells. The antigen is expressed by the villi of the intestine. Capillaries of the blood brain barrier seem to express the antigen. It is very suitable for in vitro monitoring of M-CSF stimulated bone marrow cell cultures, as the antigen is gradually expressed with macrophage development.

Use: -Immunocytochemistry on acetone fixed or frozen sections

        -flow cytometry

Storage: Store at 2-8 Deg C. |

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


rat anti-mouse dendritic cells  clone MIDC 8

-clone MIDC is a highly suitable for the identification of interdigitating cells in secondary lymphoid organs, the medulla of the thymus and its in vitro counterparts. In combination with NLDC-145, it is very useful fot he isolation of dendritic cells from the thymus. Double staining with MIDC-8 and NLDC-145 allows a more precise characterization of cells to be studied. MIDC-8 recognizes very specific cytoplasmic components whereas NLDC-145, which is more widely distribued, stains the cytoplasma.

-ref: Eur J Immunol 17, 1555 (1987)

-rat IgG2a

-suitable for frozen sections

-cat#RDI-T2014X  $531.00/200ug


rat anti-mouse DEC-205 (interdigitating cells) clone NLDC-145

-Clone NLDC-145 is a useful marker for the identification of Ia positive interdigitating cells and their in vitro counterparts. Double staining with MIDC-8 and NLDC-145 allows a more precise characterization of cells to be studies. MIDC-8 recognizes very specific cytoplasmic components whereas NLDC-145, which is more widely distributed, stains the cytoplasm (after fixation) and cell surface.

-ref: J Exp Med 163, 981 (1986)

-rat IgG2a

-cat#RDI-T2013X      $594.00/100 tests

-cat#RDI-T2025X     $656.00/50ug Biotin conjugated

cat#RDI-T2023X    $656.00/200ug FITC conjugated 

NLDC-145

Product Information

Anti Nonlymphoid Dendritic Cells (DEC-205)

NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.

Application: immunohistology, FACS

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Purity: Purified IgG from culture supernatant

Physical State: liquid

Quantity: 100ug in 100ul

Concentration: 1mg/ml

Buffer: PBS, pH 7.2

Stabilizer: none

Preservative: 0.1% sodium azide

Storage: 4°C for 6 months

Long term storage: aliquot and freeze at -20°C or lower

Do not freeze working dilutions.

Approximate working dilution: 0.5mg/ml (1:400) but depends on the system used. Optimal concentration should be tested by serial dilutions

Fixation: Acetone 10 min

For laboraty use and research purposes only.

Caution:this product contains sodium azide, a poisonous and hazardous substance.

SELECTED REFERENCES

Kraal, G., M. Breel, M. Janse, G. Bruin: Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med 163: 981-987 (1986)

Breel, M., R.E. Mebius, G. Kraal: Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol 17: 1555-1559 (1987).

Swiggard, W.J., A. Mirza, M.C. Nussenzweig, R.M. Steinman: DEC-205, a 205kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cellular Immunology 165, 302-311 (1995).

Martinez del Hoyo, G., P. Martín, H. Hernández Vargas, S. Ruiz, C. Fernández Arias, C. Ardavín: Characterization of a common precursor population for dendtiric cells. Nature 415: 1043-47 (2002)

BIOLOGICAL CHARACTERISTICS

Specificity

Mouse: nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.

Other species: unknown

Biochemistry

The antigen is a protein of 205kDa molecular weight (DEC-205) which is localized on the cell surface and intracellularly.

Antigen Distribution:

Organ NLDC-145 staining Cell type and localization

Spleen + IDC in inner PALS

Lymph node + IDC in paracortex

VC in subcapsular sinus

Peyer's patch + IDC in interfollicular T cell areas

Villum epithelium, isolated cells in submucosa (VC)

Thymus + IDC in medulla

Cortical epithelium

Skin + Langerhans cells

Brain, Kidney, Liver, Heart -

In vitro isolated cells

Blood, bone marrow -

Peritoneal cells -

Peritoneal exudate cells* + some positive cells (VC?)

* Peritoneal exudate cells were harvested 4 days after intraperitoneal thioglycollate injection.

Tests were carried out on BALB/c and C3D2F1 mouse strains.

(G.Kraal et al. see ref. 1, modified)


rat anti-mouse Histiocytes  clone EP-MP23

-clone ER-MP23 is a very sueful marker for the detection of murine connective tissue macrophages (histiocytes)

-suitable for frozen sections or glutaraldehyde sections

-rat IgG2a

-cat#RDI-2009X   $594.00/100ug

ER-MP 23

Product Information

Anti mouse macrophage glactose specific lectin (MMGL)

ER-MP23 detects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).

Application: Immune Histology, FACS

Lot number:

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Purity / Physical State Purified / Freeze-dried

Quantity 100ug

Reconstitute in: 0.5 ml distilled water (=isotonic stock solution)

Buffer: Phosphate buffered saline pH 7.2

Stabilizer: 10mg/ml bovine serum albumin

Preservative: 0.01% Thimerosal

Storage: unreconstituted: 4°C for 1 year

after reconstitution: aliquot and freeze at -20°C or lower

do not freeze working dilutions

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 1mg/ml (1:200) freshly prepared

Fixation: Acetone

For laboratory use and research purposes only. Caution: this product contains Thimerosal a hazardous and dangerous substance!

SELECTED REFERENCES

Jansen A. et al.: Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and b-cell destruction in NOD mice. Diabetes 43: 667-675 (1994)

BIOLOGICAL CHARACTERISTICS

SPECIFICITY

Mouse: Macrophages of connective tissues

Other: unknown

Immunogen: mouse macrophage cell lines

ANTIGEN The antigen is a glutaraldehyde (0.05%) resistant 38kD single chain surface glycoprotein

ANTIGEN DISTRIBUTION

Isolated cells:

The antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6, RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen .

Tissue sections:

The antigen is abundant on mature macrophages in connective tissues as present in the skin (dermis), salivary glands, capsule of lymph nodes, thymus and various other organs. The antigen is partly coexpressed with the BM8 antigen. ER-MP23 positive macrophages may express Ia antigens.


rat anti-mouse macrophage scavenger receptor clone 2F8

-cat#RDI-T2123X   $594.00/250ug

-clone 2F8 recognizes the murine scavenger receptor types I and II on tissue macrophages. It inhibits the uptake of acetylated LDL as well as divalent cation independent adhesion

-suitable for frozen sections and FACS

-ref: Nature 364, 343 (1993)

-rat IgG2b

Product Information

Rat anti-mouse macrophage scavenger receptor

Monoclonal antibody 2F8 recognises the murine scavenger receptor types I and II. It inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. The molecule recognised is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues, e.g. atherosclerotic lesions.

Applications: immunohistology on frozen but not paraffin embedded sections, ELISA, Western Blotting, immunoprecipitation, FACS.

Lot number:

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2b

Quantity / Volume: 0.25mg / 0.25ml

Purity: Purified IgG from tissue culture supernatant

Physical state: liquid

Buffer: Phosphate buffered saline, pH 7.4

Preservative: 0.1% sodium azide

Stabilizer: none

Storage: Aliquots of stock solution can be kept frozen at -70°C;

Do not freeze working dilutions.

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 1:50 - 1:100

Caution: this product contains sodium azide, a poisonous and hazardous substance

For laboratory use and research purposes only

REFERENCES:

Fraser I.P. et al.: Nature 364: 343-46 (1993)

de Villiers W.J.S. et al.: J. Exp. Med. 180: 705-709 (1994)

Hughes D.A. et al.: Eur. J. Immunol. 25: 466-73

Bell M.D. et al.: J. Neurocytol. 23: 605-613 (1994)

Hughes, D.A. et al.: Imm. Lett. 43: 7-14 (1994)


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phone (978) 371-6446 or (800) 370-2222

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EMAIL:antibodies@fitzgerald-fii.com

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