rev: May 14, 2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Specialty anti-mouse Macrophage related
-see also mouse CD reactive antibodies
Comparison chart of different mature macrophage markers
|
|
F4/80 |
ER-BMDM1 |
||
|
monocytes |
+ |
+ |
+ |
- |
|
kupffer cells |
+ |
+ |
+ |
- |
|
langerhans cells |
+- |
+ |
+ |
|
|
tingible body macrophages |
+ |
- |
- |
|
|
interdigitating cells |
+- |
- |
- |
+ |
|
dendritic cells |
+- |
- |
- |
+ |
|
microglial cells |
- |
+ |
- |
- |
|
marginal zone macrophages |
- |
- |
- |
? |
|
marginal metallophillic cells |
- |
- |
- |
- |
|
pneumocytes type II |
|
|
|
+ |
|
alveolar lavage cells |
|
41% |
66% |
26% |
|
resident perinotoneal cells |
|
47% |
51% |
34% |
|
thioglycollate elicited peritoneal cells time after injection 8 4hours |
|
17% 73% |
28% 81% |
15% 79% |
|
bone marrow cells |
14% |
27% |
37% |
5% |
|
bone marrow cells after 7 days in culture supplemented with M-CSF |
30% |
90% |
96% |
91% |
DATA SHEET:Rat anti-mouse Macrophages, monoclonal clone CI:A3-1
Catalog#: RDI-T-2008X $625.00
CI:A3-1
Product Information
Murine F4/80 antigen (Monocytes / Macrophages)
CI:A3-1 recognizes the F4/80 antigen, a 120-160kD glycoprotein containing 7 EGF-like domains at the N-terminus, an RGD (Arg-Gly-Asp) integrin binding motif, and significant homology to the transmembrane 7 (Tm7) hormone receptor family at the C-terminus. The molecular structure suggests multiple ligands. Possibly, F4/80 is involved in macrophage adhesion within certain tissues, combined with receptor signaling following peptide-ligand interaction. The antigen is expressed by most macrophages and macrophage precursors beyond M-CFC, and increases upon maturation. Activated macrophages, and eosinophils express low levels. Freshly isolated spleen dendritic cells are uniformly positive for F4/80, but the expression decreases upon culture. This antibody is very useful for characterising tissue fixed macrophages, particularly in combination with BM 8 (product RDI-T2006X) and MOMA-2 (product RDI-T2007X, new cat#: 10R-M120a $624.75/200ug).
Applications: Immunohistology on frozen and paraffin embedded sections, FACS, Immunoprecipitation, Western Blot.
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2b
Purity: Purified from culture supernatant
Physical state: Liquid
Quantity: 500ug in 500ul PBS, pH 7.2
Preservative: 0.1% Sodium azide
Storage and Stability: 1 month at 4°C, for longer periods at -20°C
Approximate working dilution: 2mg/ml on cryosections, 5mg/ml on paraffin sections, 10ml stock solution to stain 106 cells. Optimal concentration should be tested by serial dilutions.
This product requires trypsinisation of formalin fixed tissues.
Caution: this product contains sodium azide, a poisonous and hazardous substance For laboratory use and research purposes only
SELECTED LITERATURE
Hume, D. et al.: The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen. J. Exp. Med.: 158, 1522 - 1536 (1983).
Felix, R.et al.: Impairment of Macrophage Colony-stimulating Factor Production and lack of resident bone Marrow Macrophages in the osteoporotic op/op Mouse. J. Bone & Mineral Res.: 5/7, 781 - 789, (1990).
Sunderkotter, C. et al.: Cellular events associated with Inflammatory Angiogenesis int the Mouse Cornea. Am. J. Path. 138, 931 - 939, (1991).
Kraal, G. et al.: Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2; An Immunohistochemical Study. Scand. J. Immunol.: 26, 653 - 661 (1987).
BIOLOGICAL CHARACTERISTICS
Specificity
Mouse: monocytes, macrophages
Other species: unknown
BIOCHEMISTRY
CI:A3-1 detects a 160kD membrane protein of the F4/80 antigen.
ANTIGEN DISTRIBUTION
Isolated cells:
F 4/80 is recognised on monocytes of the peripheral blood and the bone marrow.
Tissue sections:
The determination of F 4/80 is already widely used for the detection of tissue macrophages. F4/80 positive macrophages, however, consist of different subpopulations from those detected with BM 8 or MOMA-2.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET:Rat anti-mouse PAN monocyte/Macrophages, monoclonal clone MOMA-2
RDI-T2007X $470.00 Monoclonal Antibody
Product Information
Anti Murine Monocytes / Macrophages
MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with BM8 and F 4/80 it allows a more precise characterisation of tissue fixed macrophages of various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface.
Application: Immunohistology on fresh frozen tissues, FACS (permeabilisation preferable)
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2b
Quantity 200mg
Volume 200ml
Purity: Purified from tissue culture supernatant
Physical state: liquid
Buffer: Phosphate buffered saline pH 7.2
Preservative: 0.1% Sodium azide
Stabilizer: none
Storage and Stability: 1 month at 4°C, for longer periods at -20°C
Approximate working dilution: 0.5mg/ml (1:1600)
Fixation: Dry acetone, 4°C.
Note: Only fresh frozen tissues should be used for MOMA-2.
Caution: this product contains sodium azide, a poisonous and hazardous substance. For laboratory use and research purposes only.
SELECTED LITERATURE
BREEL,M., MEBIUS,R.E., KRAAL,G.: Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur. J. Immunol.: 17, 1555 - 1559 (1987).
KRAAL,G., REP,M., JANSE,M.: Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2; An Immunohistochemical Study. Scand. J. Immunol.: 26, 653 - 661 (1987).
BIOLOGICAL CHARACTERISTICS
SPECIFICITY
Mouse: monocytes, macrophages
Other species: unknown
BIOCHEMISTRY
MOMA-2 detects a (glyco-)protein of 140kD m.w. which is located within the cytoplasm and on the cell surface. Attempts to isolate the antigenic determinant by immunoprecipitation and immunoblotting have so far been unsuccessful.
ANTIGEN DISTRIBUTION
Isolated cells:
In the cytospin preparation of thiogycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells
Tissue sections:
MOMA-2 detects typical tissue macrophages as do F4/80 or BM8. However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where F 4/80 and BM8 are negative.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET:Rat anti-mouse Pan Tissue Fixed Macrophages, monoclonal clone BM8
cat#RDI-T2006X, new cat#: 10R-M156b $624.75
Product Information
Anti Murine F4/80 - Pan Macrophages
BM8 recognizes the F4/80 antigen on major subpopulations of resident tissue macrophages. The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model.
Applications: Histology on frozen and paraffin embedded sections, FACS
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2a
Purity Affinity Purified
Quantity: 100µg
Buffer: Phosphate buffered saline, pH 7.2
Stabilizer: 10mg/ml bovine serum albumin
Preservative: 0.1% sodium azide
Reconstitute in: 0.5 ml distilled water (=stock solution)
Storage: unreconstituted: 4°C for 1 year
after reconstitution: aliquot and freeze at -20°C or lower
do not freeze working dilutions!
Stability of stock solution: approx. 1 year at -70°C
Approximate working dilution: optimal concentration should be tested by serial dilution
Histology 0.5mg/ml (1:400) freshly prepared on frozen sections
Fixation: Acetone, Glutaraldehyde, Formaldehyde / Paraffin
This product requires proteinase treatment of paraffin sections.
Caution: this product contains sodium azide, a poisonous and hazardous substance. For laboratory use and research purposes only.
SELECTED REFERENCES
Schaller, E. et al.: Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol. Cell Biol. 22: 8035-43 (2002).
Malorny, U. et al.: A monoclonal antibody angainst an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 243, 421-428 (1986).
Kraal, G. et al.: Macrophages in T and B Cell compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2......... Scand. J. Immunol. 26, 653-661 (1987).
Leenen, P.J.M. et al.: Markers of mouse macrophage development detected by monoclonal antibodies. J. Immunol. Methods, 174: 5-19 (1994).
Smit, M.J. et al.: Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells............ Hepatology 12: 1192-1199 (1990)
Rosmalen, J.G.M. et al.: Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates....... Lab. Invest. 80: 23-30 (2000)
BIOLOGICAL CHARACTERISTICS SPECIFICITY
Mouse: major subpopulation of resident tissue macrophages
Other species: human heart macrophages
BIOCHEMISTRY
The antigen is a 125kD extracellular membrane protein sensitive to 2-mercaptoethanol.
ANTIGEN DISTRIBUTION
Isolated Cells:
The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.
Tissue Sections:
The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals
or for diagnostics. Sodium azide may form explosive compounds in presence of
heavy metals or under acidic conditions. Flush drains with copious amounts of
water to prevent buildup of explosive compounds.It is the responsibility of the
user to comply with all local/state and Federal rules in the use of this
product. We are not responsible for any patent infringements that might result
with the use of or derivation of this product.
-also available:
Anti Murine Pan Macrophages Clone BM8 Biotin conjugated cat#RDI-T2017X $656.00/150ug
BM8 is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model.
Applications: Histology on frozen and paraffin embedded sections, FACS
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2a
Purity Affinity Purified
Quantity: 150µg
Buffer: Phosphate buffered saline, pH 7.2
Stabilizer: 10mg/ml bovine serum albumin
Preservative: 0.01% Thimerosal
Reconstitute in: 0.5 ml distilled water (=stock solution)
Storage: unreconstituted: 4°C for 1 year
after reconstitution: aliquot and freeze at -20°C or lower
do not freeze working dilutions
Stability of stock solution: 1 year at -70°C
Approximate working dilution: optimal concentration should be tested by serial dilution
Histology 0.5mg/ml (1:800)
Fixation: Acetone, Glutaraldehyde, Formaldehyde / Paraffin
Caution: this product contains Thimerosal, a poisonous and hazardous substance. For laboratory use and research purposes only.
SELECTED REFERENCES
Malorny, U. et al.: A monoclonal antibody angainst an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 243, 421-428 (1986).
Kraal, G. et al.: Macrophages in T and B Cell compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2, an Immunohistochemical Study. Scand. J. Immunol. 26, 653-661 (1987).
Malorny, U. et al.: Immunohistochemical demonstration of migration inhibitory factor (MIF) in experimental allergic contact dermatitis. Clin. Exp. Immunol. 71, 164-170 (1988).
Leenen, P.J.M. et al.: Markers of mouse macrophage development detected by monoclonal antibodies. J. Immunol. Methods, 174: 5-19 (1994).
Smit, M.J. et al.: Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells involved in receptor-mediated endocytosis of lactate dehydrogenase and other enzymes. Hepatology 12: 1192-1199 (1990)
Rosmalen, J.G.M. et al.: Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates: Correlation with the Development of Diabetes. Lab. Invest. 80: 23-30 (2000)
BIOLOGICAL CHARACTERISTICS SPECIFICITY
Mouse: major subpopulation of resident tissue macrophages
Other species: human heart macrophages
BIOCHEMISTRY
The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2-mercaptoethanol.
ANTIGEN DISTRIBUTION
Isolated Cells:
The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.
Tissue Sections:
The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
DATA SHEET:Rat anti-mouse Mid stage Macrophage Precursor Cell , monoclonal
CLONE ER-MP54 Catalog#: RDI-T2003X, new cat#: 10R-M153a $624.75
ER-MP 54
Product Information
Murine Early Stage Myeloid Precursor Cells
ER-MP54 is a general marker for detecting myeloid precursor cells in the bone marrow. The antigen is detected on a range of macrophage precursor cells up to the monoblast level.
Application: Histology, FACS
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG1
Purity /
Quantity 100ug
Buffer: PBS pH 7.2
Stabilizer: 10mg/ml bovine serum albumin
Preservative: 0.01% Thimerosal
Reconstitute in: 0.5ml distilled water (=isotonic stock solution)
Storage: unreconstituted: 4°C for 1 year
after reconstitution: aliquot and freeze at -20°C or lower
do not freeze working dilutions!
Stability of stock solution: approx. 1 year at -70°C
Approximate working dilution: 1mg/ml (1:300) freshly prepared
Fixation: Acetone, 0.05% Glutaraldehyde
For laboratory use and research purposes only. Caution: this product contains Thimerosal a hazardous and dangerous substance!
SELECTED REFERENCES
Leenen, P.J.M., Melis, M., Slieker, W.A.T, Van Ewijk, W.: Murine Macrophage Precursor Characterization II. Monoclonal Antibodies against Macrophage Precursor Antigens. Eur. J. Immunol. 20: 27-34 (1990).
Leenen, P.J.M., Melis, M., Slieker, W.A.T, Van Ewijk, W.: Murine Macrophage Precursor Characterization I. Production, phenotype and differentiation of macrophages precursor hybrids J. Immunol. 20: 15-25 (1990).
Leenen, P.M.J., M.F.T.R. de Bruijn, J.S.A. Voerman, P.A. Campbell, W. van Ewijk: Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Meth 174: 5-19 (1994)
BIOLOGICAL CHARACTERISTICS
Specificity:
Mouse: Myeloid precursors up to monoblats, cytoplasmatic antigen
Other: unknown
Immunogen:
Mouse macrophage cell lines, where the antigen is detected on the cell surface.
Antigen:
Multiple antigens were precipitated by ER-MP54 under reducing and non-reducing conditions: Major bands could be detected at 90, 85-80 and 75-70 kDa, at lower amounts at 45kDa and less frequently at 27 and 12 kDa.
Antigen Distribution:
Isolated cells:
The antigen is present on bone marrow myeloid cells up to monoblasts where the antigen is localized in the cytoplasm. Under specific experimental in vitro conditions, treatment by glucocorticoids, the antigen can also be detected on the cell surface.
Tissue sections:
The antigen can be detected in bone marrow plugs and some clusters of red pulp in spleen.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET: Rat
anti-mouse Hematopoisis Associated Macrophages, monoclonal
Catalog#: RDI-T2012X Price: $594.00/vial
Package Size: 500ug
Supplied: purified
Clone: ER HR-3
Ig Isotype: Rat IgG2c
Reactivity: Clone ER HR3 is a useful marker for the identification and localization of a very distinct macrophage sub-population found in various organs. This marker is especially suitable for ontogenetic studies because ER-HR3 positve macrophages are cosely related to hemopoietic islands, especially at erythropoietic sites.
Use: -Immunocytochemistry on formalin fixed paraffin or acetone fixed or frozen sections.
-flow cytometry
ref: Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis, Vol
H8, p.237, I.N. Rich ed., Springer Verlag (1987)
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federalrules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET:Rat anti-mouse Splenic Marginal Zone Macrophages, monoclonal
Catalog#: RDI-T2010X Price: $594.00/vial
Package Size: 150ug
Supplied: purified
Clone: ER TR 9
Ig Isotype: Rat IgM
Reactivity: Clone ER TR 9 is useful for the identification of macrophage subpopulations present in the splenic marginal zone, lymph medulla and other organs. In combination with clone MOMA-1, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER TR 9 is also useful when studying phagocytosis of neutral polysaccharides as the antibody selectively inhibits uptake of these glycans by macrophages.
Use: -Immunocytochemistry on acetone fixed tissues
-flow cytometry
ref: J. Histochem. Cytochem. 33, 40 (1985)
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET: Rat anti-mouse Pan Tissue Fixed Macrophages, monoclonal MOMA-1
Catalog#: RDI-T2011X, new cat#: 10R-M156a Price: $624.75/vial
-also available Biotin conjugated cat#RDI-T2021X, new cat#: 61R-M146aBT $693.00/200ug
MOMA 1
Product Information
Murine metallophilic macrophage marker
MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by ER-TR 9. In addition , MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF).
Application: Immune histology
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2a
Purity / Physical State Purified / lyophilized
Quantity 200ug
Reconstitute in: 0.5 ml distilled water (=isotonic stock solution)
Buffer: Phosphate buffered saline pH 7.2
Stabilizer: 10mg/ml bovine serum albumin
Preservative: 0.01% Thimerosal
Storage: unreconstituted: 4°C for 1 year after reconstitution: aliquot and freeze at -20°C or lower do not freeze working dilutions.
Stability of stock solution: 1 year at -70°C
Approximate working dilution: 4mg/ml (1:100) freshly prepared
Fixation: Acetone
For laboratory use and research purposes only. Caution: this product contains Thimerosal, a hazardous and dangerous substance!
Literature:
Kraal, G., Janse, M.: Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. Immunology 58, 665-669 (1986).
BIOLOGICAL CHARACTERISTICS
SPECIFICITY
Mouse: Subpopulation of mature resident tissue macrophages
Other: unknown
Immunogen: mouse macrophage cell lines
ANTIGEN
The antigen is a cytoplasmic and cell surface component.
ANTIGEN DISTRIBUTION
Isolated cells:
No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
Tissue sections:
Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the inerfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET: Rat anti-mouse Macrophage-aminopeptidase), monoclonal (CD13?)
Catalog#: RDI-MS-ERBMBM1
Price: $562.00/vial
Package Size: 100 tests
Clone: ER-BMDM1
Ig Isotype: Rat IgG2a
Reactivity: Clone ER-BMDM1 is a useful for in situ and in vitro identification
of aminopeptidase N positive macrophages, interdigitating cells and dendritic
cells. The antigen is expressed by the villi of the intestine. Capillaries of
the blood brain barrier seem to express the antigen. It is very suitable for in
vitro monitoring of M-CSF stimulated bone marrow cell cultures, as the antigen
is gradually expressed with macrophage development.
Use: -Immunocytochemistry on acetone fixed or frozen sections
-flow cytometry
Storage: Store at 2-8 Deg C. |
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or
derivation of this product.
rat anti-mouse dendritic cells clone MIDC 8
-clone MIDC is a highly suitable for the identification of interdigitating cells in secondary lymphoid organs, the medulla of the thymus and its in vitro counterparts. In combination with NLDC-145, it is very useful fot he isolation of dendritic cells from the thymus. Double staining with MIDC-8 and NLDC-145 allows a more precise characterization of cells to be studied. MIDC-8 recognizes very specific cytoplasmic components whereas NLDC-145, which is more widely distribued, stains the cytoplasma.
-ref: Eur J Immunol 17, 1555 (1987)
-rat IgG2a
-suitable for frozen sections
-cat#RDI-T2014X $531.00/200ug
rat anti-mouse DEC-205 (interdigitating cells) clone NLDC-145
-Clone NLDC-145 is a useful marker for the identification of Ia positive interdigitating cells and their in vitro counterparts. Double staining with MIDC-8 and NLDC-145 allows a more precise characterization of cells to be studies. MIDC-8 recognizes very specific cytoplasmic components whereas NLDC-145, which is more widely distributed, stains the cytoplasm (after fixation) and cell surface.
-ref: J Exp Med 163, 981 (1986)
-rat IgG2a
-cat#RDI-T2013X $594.00/100 tests
-cat#RDI-T2025X $656.00/50ug Biotin conjugated
cat#RDI-T2023X, new cat#: 51R-D120aFT $693.00/200ug FITC conjugated
NLDC-145
Product Information
Anti Nonlymphoid Dendritic Cells (DEC-205)
NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.
Application: immunohistology, FACS
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2a
Purity: Purified IgG from culture supernatant
Quantity: 100ug in 100ul
Concentration: 1mg/ml
Buffer: PBS, pH 7.2
Stabilizer: none
Preservative: 0.1% sodium azide
Storage: 4°C for 6 months
Long term storage: aliquot and freeze at -20°C or lower
Do not freeze working dilutions.
Approximate working dilution: 0.5mg/ml (1:400) but depends on the system used. Optimal concentration should be tested by serial dilutions
Fixation: Acetone 10 min
For laboraty use and research purposes only.
Caution:this product contains sodium azide, a poisonous and hazardous substance.
SELECTED REFERENCES
Kraal, G., M. Breel, M. Janse, G. Bruin: Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med 163: 981-987 (1986)
Breel, M., R.E. Mebius, G. Kraal: Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur J Immunol 17: 1555-1559 (1987).
Swiggard, W.J., A. Mirza, M.C. Nussenzweig, R.M. Steinman: DEC-205, a 205kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cellular Immunology 165, 302-311 (1995).
Martinez del Hoyo, G., P. Martín, H. Hernández Vargas, S. Ruiz, C. Fernández Arias, C. Ardavín: Characterization of a common precursor population for dendtiric cells. Nature 415: 1043-47 (2002)
BIOLOGICAL CHARACTERISTICS
Specificity
Mouse: nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells.
Other species: unknown
Biochemistry
The antigen is a protein of 205kDa molecular weight (DEC-205) which is localized on the cell surface and intracellularly.
Antigen Distribution:
Organ NLDC-145 staining Cell type and localization
Spleen + IDC in inner PALS
Lymph node + IDC in paracortex
VC in subcapsular sinus
Peyer's patch + IDC in interfollicular T cell areas
Villum epithelium, isolated cells in submucosa (VC)
Thymus + IDC in medulla
Cortical epithelium
Skin + Langerhans cells
Brain, Kidney, Liver, Heart -
In vitro isolated cells
Blood, bone marrow -
Peritoneal cells -
Peritoneal exudate cells* + some positive cells (VC?)
* Peritoneal exudate cells were harvested 4 days after intraperitoneal thioglycollate injection.
Tests were carried out on BALB/c and C3D2F1 mouse strains.
(G.Kraal et al. see ref. 1, modified)
rat anti-mouse Histiocytes clone EP-MP23
-clone ER-MP23 is a very sueful marker for the detection of murine connective tissue macrophages (histiocytes)
-suitable for frozen sections or glutaraldehyde sections
-rat IgG2a
-cat#RDI-2009X $594.00/100ug
ER-MP 23
Product Information
Anti mouse macrophage glactose specific lectin (MMGL)
ER-MP23 detects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).
Application: Immune Histology, FACS
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2a
Purity /
Quantity 100ug
Reconstitute in: 0.5 ml distilled water (=isotonic stock solution)
Buffer: Phosphate buffered saline pH 7.2
Stabilizer: 10mg/ml bovine serum albumin
Preservative: 0.01% Thimerosal
Storage: unreconstituted: 4°C for 1 year
after reconstitution: aliquot and freeze at -20°C or lower
do not freeze working dilutions
Stability of stock solution: 1 year at -70°C
Approximate working dilution: 1mg/ml (1:200) freshly prepared
Fixation: Acetone
For laboratory use and research purposes only. Caution: this product contains Thimerosal a hazardous and dangerous substance!
SELECTED REFERENCES
Jansen A. et al.: Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and b-cell destruction in NOD mice. Diabetes 43: 667-675 (1994)
BIOLOGICAL CHARACTERISTICS
SPECIFICITY
Mouse: Macrophages of connective tissues
Other: unknown
Immunogen: mouse macrophage cell lines
ANTIGEN The antigen is a glutaraldehyde (0.05%) resistant 38kD single chain surface glycoprotein
ANTIGEN DISTRIBUTION
Isolated cells:
The antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6, RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen .
Tissue sections:
The antigen is abundant on mature macrophages in connective tissues as present in the skin (dermis), salivary glands, capsule of lymph nodes, thymus and various other organs. The antigen is partly coexpressed with the BM8 antigen. ER-MP23 positive macrophages may express Ia antigens.
rat anti-mouse macrophage scavenger receptor clone 2F8
-cat#RDI-T2123X, new cat#: 10R-M103a $593.25/250ug
-clone 2F8 recognizes the murine scavenger receptor types I and II on tissue macrophages. It inhibits the uptake of acetylated LDL as well as divalent cation independent adhesion
-suitable for frozen sections and FACS
-ref: Nature 364, 343 (1993)
-rat IgG2b
Product Information
Rat anti-mouse macrophage scavenger receptor
Monoclonal antibody 2F8 recognises the murine scavenger receptor types I and II. It inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. The molecule recognised is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues, e.g. atherosclerotic lesions.
Applications: immunohistology on frozen but not paraffin embedded sections, ELISA, Western Blotting, immunoprecipitation, FACS.
TECHNICAL CHARACTERISTICS
Species: Rat
Class/Subclass: IgG2b
Quantity / Volume: 0.25mg / 0.25ml
Purity: Purified IgG from tissue culture supernatant
Physical state: liquid
Buffer: Phosphate buffered saline, pH 7.4
Preservative: 0.1% sodium azide
Stabilizer: none
Storage: Aliquots of stock solution can be kept frozen at -70°C;
Do not freeze working dilutions.
Stability of stock solution: 1 year at -70°C
Approximate working dilution: 1:50 - 1:100
Caution: this product contains sodium azide, a poisonous and hazardous substance
For laboratory use and research purposes only
REFERENCES:
Fraser I.P. et al.: Nature 364: 343-46 (1993)
de Villiers W.J.S. et al.: J. Exp. Med. 180: 705-709 (1994)
Hughes D.A. et al.: Eur. J. Immunol. 25: 466-73
Hughes, D.A. et al.: Imm. Lett. 43: 7-14 (1994)
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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