rev:    October 5, 2004

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Mouse anti-MGMT   (06-methylguanine-DNA methyltransferase)

cat# RDI-MGMTabm        $438.00/100ug

        RDI-MGMTabm-1    $688.00/200ug

        RDI-MGMTabm-XP $750.00/200ug

        RDI-MGMTabm-BT  $500.00/100ug Biotin conjugated

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Clone: Mt 3.1

aa: region 8-221

Isotype: IgG1

Immunogen: Purified recombinant human MGMT protein expressed in E. coli.

Hybridoma: Produced by fusion between BALB/c splenocytes and myeloma p3X63/Ag8.653 cells.

Use: -Flow Cytometry

- Immunoprecipitation (2ug/mg protein) use Protein G (reported)

-Western Blotting 0.5-1ug/ml 2hrs RT with chemiluminescence detection

-Immunohistology (Frozen & formalin-fixed) **

(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.) approx 2-5ug/ml with 60 minute RT incubation, ABC/Dab detection

-all titers must be optimized for each use

Species Reactivity: Human. Also reacts weakly with Mouse, Rat, and Hamster. Others-not tested.

Positive Control: CCRF-CEM,1 Molt-4,1 Raji,1 HeLa CCL2,1 Rh18, 1 or HT-29 2 cells. Liver tissue expresses high levels while brain and bone marrow have low levels of MGMT protein.

Cellular Localization: Nuclear and cytoplasmic

Background: It recognizes a protein of 25kDa, identified as MGMT (O 6 -methylguanine-DNA methyltransferase). Cancer chemotherapeutic alkylating agents (e.g. BCNU, CCNU, DTIC, procarbazine, temozolomide), act by inducing formation of lethal cross-links at the O 6 - alkylguanine position in DNA. MGMT transfers alkyl adducts from the O 6 -position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA- damage it can tolerate. Because MGMT repairs the potentially cytotoxic, antitumor lesions induced in DNA by alkylating agents, tumors with low or no levels of MGMT will predictably be responsive to such drug   therapy. Conversely, tumors with high levels of MGMT will likely be drug resistant. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Abnormally low levels of MGMT in normal individuals/tissues may be an early marker of susceptibility to cancer.

ref: 1. Harris LC, et. al. Cancer Research, 1992, 52(22):6404-6.

2. von Wronski MA, et. al. Carcinogenesis, 1994, 15(4):577-82.

Storage: 2-8 deg c

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


or (800) 370-2222

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